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Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 172
4 year old spores germinate after 2 mothes. * 1
    #2858378 - 07/05/04 09:17 AM (19 years, 8 months ago)

Just to let all you guys know, Mr. Endo has sent me a pic of an agar plate that was inoculated with EQ spores on 25/4/04 (as seen in the pic). He told me these spores were not kept in good conditions occaisoanly reaching quite hot temperatures.
After 2 monthes of inoculation these spores began to grow and allready cloned, according to Mr. Endo...


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Trading omelettes is fun..... :P

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Offlinedebianlinux
Myconerd - DBK
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Registered: 12/09/02
Posts: 8,334
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Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2858559 - 07/05/04 10:59 AM (19 years, 8 months ago)

okay... i'll bite since it's so quiet.

a pressing question comes to mind; by your saying "cloned" am i to infer that what i see on the pictured plate was successfully fruited?

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Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 172
Re: 4 year old spores germinate after 2 mothes. [Re: debianlinux]
    #2858759 - 07/05/04 12:48 PM (19 years, 8 months ago)

in cloned i meant to say isolated from the first generation petri dish...


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Trading omelettes is fun..... :P

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Offlinedebianlinux
Myconerd - DBK
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Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2858801 - 07/05/04 01:08 PM (19 years, 8 months ago)

the long germination period may have been a result of dehydrated spores. while dehydration shouldn't necessarily affect vitality it does introduce a rehydration period.

once visible mycelia was established did it seem to grow out at an acceptable speed or was impairment noticable?

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OnlineDERRAYLD
Constructus
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Registered: 05/13/02
Posts: 9,839
Loc: South Africa
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Re: 4 year old spores germinate after 2 mothes. [Re: debianlinux]
    #2860230 - 07/05/04 11:22 PM (19 years, 8 months ago)

Yes, I have also just germinated some 3 1/2 yr old PR spores.
The germination is no real issue, just taking longer.
It`s the actual rate of mycelial growth that tends to be far slower.
Fruiting the culture will most likely yield a new spore print for future use.

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Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 172
Re: 4 year old spores germinate after 2 mothes. [Re: DERRAYLD]
    #2861164 - 07/06/04 10:25 AM (19 years, 8 months ago)

OK M.E. told me the OLD EQ mycelium grows at a regular rate now, but, the mycelium fails to give out any rhizomorphic lines, though he is still hoping for it to fruit...


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Trading omelettes is fun..... :P

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OfflinePrankster239
Spore Searcher
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Registered: 01/06/04
Posts: 518
Loc: Europe
Last seen: 1 year, 6 months
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2868380 - 07/08/04 10:02 AM (19 years, 8 months ago)

It is mostly the best to try old spores on liquid media or put them in steril water for a few days.


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OfflineKillBill
Quick Learner
Registered: 07/02/04
Posts: 109
Last seen: 19 years, 5 months
Re: 4 year old spores germinate after 2 mothes. [Re: Prankster239]
    #2949983 - 07/31/04 08:20 PM (19 years, 7 months ago)

haha I am currently working with 4 year old spores as a matter of fact, i had two out of four Brf jars showed nothing,and a rye showed nothing, I wonder if they're showed activity in a month or so?

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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2952230 - 08/01/04 02:34 PM (19 years, 7 months ago)

A true testament to the hardy nature of the tiny spore.


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What it is, is what it is my Brother.
It is as it is, so suffer thru it.

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Offlinehyphae
born to grow
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Registered: 12/13/02
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Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2969743 - 08/05/04 08:14 PM (19 years, 7 months ago)

I've grown out original PFC syringes that were 6+ years old. All germinated in 7-10 days! Damn those are some potent fuckers!!
Dehydration plays a big part in prints and long term storage.



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Offlinefelixhigh
Scientist
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Registered: 06/24/01
Posts: 7,565
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Re: 4 year old spores germinate after 2 mothes. [Re: hyphae]
    #2973385 - 08/06/04 07:34 PM (19 years, 7 months ago)

there are strains that i only find a print each 4-5 years (what a mess of prints i know) and they start just like any non-fresh print... (sometimes the germ is real quick)
and i've used no fridge, just stored it sealed of light and air... (no vaccum though)...
it works wonders...
btw, classic PFs! :wink:


FH

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: 4 year old spores germinate after 2 mothes. [Re: felixhigh]
    #2973671 - 08/06/04 08:45 PM (19 years, 7 months ago)

No dehydration after 4-5yrs.? Wow that is amazing! :wink: Ya I don't store any prints in the fridge myself either :smile:

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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2979296 - 08/08/04 08:30 PM (19 years, 7 months ago)

This gives me a bit of hope. I recently received some very rare spores that are 20 years old. They've been six weeks on agar and so far. . .nothing. I won't toss the dishes, but just leave them be and hope for the best. I sure would like to see these germinate.

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
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Re: 4 year old spores germinate after 2 mothes. [Re: RogerRabbit]
    #2979327 - 08/08/04 08:41 PM (19 years, 7 months ago)

Maybe placing some sterile water in the dish would help with the rehydration process, I also always break the surface of the agar when germinating spores and find 95% of the time the break is the first place to germinate. :wink: Damn 20yrs. what strain? I have several prints of the original PFC's that would be great if I decide to start them up again :laugh: Lemme know how they go RR and GL :wink:

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Invisiblefastfred
Old Hand
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: 4 year old spores germinate after 2 mothes. [Re: hyphae]
    #2980611 - 08/09/04 06:01 AM (19 years, 7 months ago)

I have had similar experiences also... The spores often need to be embedded in the agar, not just sitting on the surface.

You might try using a spreader to embed them. Or you might try an overlay with soft agar.

-FF

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Offlinedutchie
Smokin Aussie
Registered: 07/27/04
Posts: 1
Last seen: 19 years, 7 months
Re: 4 year old spores germinate after 2 mothes. [Re: fastfred]
    #2984505 - 08/10/04 01:31 AM (19 years, 7 months ago)

After begining my lost hobby once again, I have found that 3 year old mazatapec spores rehydrated and germinated in a week. Alas, The p.tamp spores did not.:( They were both of the same age. Weird. The p.cub print was far thicker tho. Both were started in 2% honey water. P.tamp spores were also wiped on agar but also no growth. Its been two months....

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