Bungmurphy said:

UPDATE 2/16/24
Just got results from latest testing. I took the same PE culture from above picture (spore syringe sourced) and ran it two different ways. The culture was put in fridge at T8 in May 2023, took out October 2023 and several transfers were made. I ran 2 56QT mono tubs, both had 4 quarts grain (WBS with some popcorn 5% and rye 5%) were spawned to 10 quarts substrate with 4 quarts of pseudocasing. The difference being one tub got just coir, verm; the second tub got coir, verm and 1/2 cup of dried manure. Substrate was pasteurized in jars on stovetop for 90+ min. I let the coir only tub go for 3 flushes, just to see max yield, and due to space issues. From that coir only tub, my largest fruit weighed 112.0 grams wet and dried to 8.3 grams (its a little to the lower right of the Sharpie past the open area). Everything was dried in a Nesco at 165 for 48 hours.

Coir only tub pic:



1. Coir only 56 quart PE tub. 4 quarts grain, 10 quarts substrate, 4 quarts pseudocasing.
Days on grain: 33 days
Days till first flush (blobs): 35 days
First flush yield (blobs, dry): 80.0 grams
Days till second flush (penis mushrooms): 14 days
Second flush yield (mushrooms, dry): 129.0 grams
Third flush yield: 33.0 grams
Total yield: 242.0 grams
Test results:
Sample consists of 1.0 gram of blobs + 1.0 gram fruits.
Psilocybin: 8.3 mg/g
Psilocin: 0.7 mg/g
No other compounds present.
Total alkaloids: 9.0 mg/g

2. Manure added 56 quart PE tub. 4 quarts grain, 10 quarts substrate, 4 quarts pseudocasing.
Days on grain: 34 days
Days till first flush (blobs): 43 days
First flush yield (blobs, dry): 69.0 grams
Days till second flush (penis mushrooms): 11 days
Second flush yield (mushrooms, dry): 122.0 grams
Total yield: 191.0 grams
Test results:
Sample consists of 1.0 gram of blobs + 1.0 gram fruits.
Psilocybin: 6.2 mg/g
Psilocin: 1.3 mg/g
No other compounds present.
Total alkaloids: 7.5 mg/g

Bungmurphy said:

UPDATE 2/11/2026, 2 year storage test results below in BLUE


Okay, so this is an update 2/11/2026:
I saved the largest fruit from the above coir-only PE tub that finished 2/2024. The fruit weighed 112.0 grams wet and 8.3 grams dry. Here is the specific mushroom in the tub before harvest:
And here is how I stored it for the last 2 years. Heat sealed in quart jar with single 2 gram desiccant packet. It was left on a bookshelf in a room that would go between mid-low 60s F to low-mid 80s F. There was no direct sunlight in the room, just regular office light LED bulbs. Jar was never opened until day of testing:


Test Results:
Norpsilocin: 0.243 mg/g
Psilocin: 1.422 mg/g
Psilocybin: 1.906 mg/g
Total alkaloids per gram: 3.571 mg/g

Obviously, the overall potency dropped, but psilocin levels seemed to increase. It's possible that the previous sample had less psilocin due to it being half fruits, half blobs. The HPLC service I use has also updated their equipment and standards in the last 2 years, so it's possible the first results were not as accurate. The potency loss over the 2 years, as stored was ~60% which tracks with other results #8 P.E. SINGLE FRUIT, from the original post above. However, that drop in potency was over 1 year and this is over 2; it's possible that potency loss has somewhat plateaued at some point.

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