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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Stimulation of axillary branching in cacti with BAP
    #2951114 - 08/01/04 08:04 AM (12 years, 4 months ago)

6-Benzylaminopurine (BAP or BA for short) is a cytokinin that stimulates branching in many plants. I normally use it in tissue culture media but i recently read something about stuff called 'keiki paste'. It's a paste that orchid growers use to stimulate the formation of keiki's (new plantlets). It would supposedly contain cytokinin.

So i made a paste of lanolin (sheep wool grease) and BAP. The lanolin was melted in a water bath and the BAP was added and mixed in. Final concentration was 1% w/w

With a toothpick a bit of the paste was smeared onto the areoles of some cacti and the results were stunning.

After two weeks all cacti show swelling of the areoles and many of them already started to sprout new shoots. I tried this with Tr. peruvianus and bridgesii but i expect it to work with most cacti.

This could be very helpful in the multiplication of rare and slow growing strains and species.

Here are two pics of Tr peruvianus that's sprouting new shoots that it normally wouldn't





If you are looking for BAP you should find a company that sells plant tissue culture supplies. They will surely have it.
Lanolin can be purchased at the pharmacy.


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Offlinefelixhigh
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2951166 - 08/01/04 09:57 AM (12 years, 4 months ago)

amazing! =D
inspiring...


FH


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OfflineOpenminded
Dicotyledon

Registered: 08/28/03
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2951183 - 08/01/04 10:17 AM (12 years, 4 months ago)

Very nice!
Coupled with a pereskiopsis graft I'm sure this could make a great way to multiply your plants...


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Offlinefelixhigh
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Re: Stimulation of axillary branching in cacti with BAP [Re: Openminded]
    #2951189 - 08/01/04 10:24 AM (12 years, 4 months ago)

yeah, our cacti don't grow out only of patience! :wink:


FH


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OfflineOpenminded
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2951495 - 08/01/04 01:11 PM (12 years, 4 months ago)

I forgot to ask - please update this regularly, I'd really like to see how this progresses.


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InvisibleStonehenge
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2951863 - 08/01/04 02:48 PM (12 years, 4 months ago)

With all the requests Una gets, he/she will have nothing else to do but answer our questions and post photos.

Grafting on peresk seems to stimulate branching more than enough. I can't see the point of using that plus the bap. Too much of a good thing is often no good at all. Imagine a plant that every areole had a pup forming. Growth would be very slow and might stop altogether as the plant tried to support all that new growth. The pups would be so jammed they'd have no room to develope. I can see using bap on plants that weren't pupping that you wanted to get some pups from. You could say I want a pup right here and make it happen. Thanks for the tip, Una.

Stoney


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OfflineOpenminded
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Re: Stimulation of axillary branching in cacti with BAP [Re: Stonehenge]
    #2952086 - 08/01/04 03:56 PM (12 years, 4 months ago)

If Una doesn't want people nagging him/her to update, he/she shouldn't make such damn interesting posts! :smile:

I'm not suggesting BAP on every aereole... But if you had, say, an aztekium, then BAP alone wouldn't be too good because the pups would take forever to grow big enough to root or graft on.
But if you had an aztekium on pereskiopsis, then stimulating three or four aereoles with BAP would ensure pup formation, and the rootstock would make them grow quickly enough to be useful.

(I don't know what pup formation is like on aztekium, it was just an example of a slow-growing cactus, more rapid cultivation of which would be desirable)


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Stimulation of axillary branching in cacti with BAP [Re: Stonehenge]
    #2952763 - 08/01/04 06:22 PM (12 years, 4 months ago)

Quote:

Grafting on peresk seems to stimulate branching more than enough. I can't see the point of using that plus the bap. Too much of a good thing is often no good at all. Imagine a plant that every areole had a pup forming. Growth would be very slow and might stop altogether as the plant tried to support all that new growth. The pups would be so jammed they'd have no room to develope. I can see using bap on plants that weren't pupping that you wanted to get some pups from. You could say I want a pup right here and make it happen.




My thoughts exactly  :thumbup:


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Offlinefaslimy
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2952861 - 08/01/04 06:39 PM (12 years, 4 months ago)

Interesting, what light source are you using for the experiment?


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Stimulation of axillary branching in cacti with BAP [Re: faslimy]
    #2952897 - 08/01/04 06:45 PM (12 years, 4 months ago)

dutch sun :smile:


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OfflineHooty
Reality isRelative

Registered: 02/24/03
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2952967 - 08/01/04 07:05 PM (12 years, 4 months ago)

Quote:

Imagine a plant that every areole had a pup forming.




I do believe such a phenomenum occurs..it's caused by some sort of microorganism, can't remember the name of the condition though...


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OfflineGumbyM
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2952983 - 08/01/04 07:08 PM (12 years, 4 months ago)

Where can someone order BAP? I want to branch the bejesus out of my pedros.


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Stimulation of axillary branching in cacti with BAP [Re: Hooty]
    #2964813 - 08/04/04 04:31 PM (12 years, 4 months ago)

>>I do believe such a phenomenum occurs..it's caused by some sort of microorganism, can't remember the name of the condition though...

Witches broom is what you mean, caused by a mycoplasma

http://cactusclinic.telenet.be/ca_mycoplasma.html


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #2985867 - 08/10/04 02:32 PM (12 years, 3 months ago)

Here's a new pic....pups are still developing :laugh:



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OfflinePsiloman
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #4023314 - 04/06/05 09:55 PM (11 years, 7 months ago)

Una do you think this would work with other plants like for example Salvia?

Well many plants come into mind.....I also think of this being applied to plants than when they mature turn into trees like Anandenanthera species.This could provide some interesting results!!!!!!

Hmmm...Im so interest in this compound: Being a purine analogue can it indce mutations? What about mutations or interestign results in mushroom cultures? Now ,these questions require some research..

I just hope i refreshed interest on this technique.Thanks for sharing!


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Invisibleeric_the_red
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Re: Stimulation of axillary branching in cacti with BAP [Re: Psiloman]
    #4023457 - 04/06/05 10:36 PM (11 years, 7 months ago)

you could stimulate axillary branching in salvia, but you would need to look up or create the correct forumla.


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Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,415
Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #4023967 - 04/07/05 12:31 AM (11 years, 7 months ago)

I have used cytokinin before on cannabis used a control my usual clones and sprayed a few to see a difference but I couldnt notice any. This may however be due to the fact that i sprayed them and they only had 2 weeks veg. No difference in flowers either but i didnt spray during flowering cause who wants to smoke that stuff.


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Invisibledurban_poison
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Re: Stimulation of axillary branching in cacti with BAP [Re: Una]
    #4024031 - 04/07/05 12:47 AM (11 years, 7 months ago)

Una you inspired me to go through my photos and find out exactly what product it was i used. damn did i grow some big tress back in the day. Well for all those intrested in my cannabis experiments with it i got at your typical nursery but havent ever seen it since but if im reading the picture right the brand is dexol it says "blossom set for tomatoes and vegtables" contains cytokinin/kinetin. for those that super crop may notice differences. considering my some what new cacti obsession i might try to track more of this down. It comes in a spray bottle so i figure i will use a q-tip to apply.


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