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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures

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Offlinebent
bent1

Registered: 07/31/03
Posts: 204
Loc: ny
Last seen: 17 years, 23 days
Syringes, Sterility question
    #2932284 - 07/27/04 11:58 AM (19 years, 4 months ago)

i have some brand new syringes, should i assemble them than use them or should i assemble them than re-sterilize them in my pressure cooker. i will be using them to suck up mycelium in honey water which i will then inject into bags of popcorn, im going to use 30ml per each 5 qt bag. basicly, should i resterilize the needles or do i not need to? also should i use a glovebox when filling the syringes, i have one, might as well use it right?


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Offlineragadinks
MrBeatle
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Registered: 10/20/03
Posts: 1,298
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Re: Syringes, Sterility question [Re: bent]
    #2933245 - 07/27/04 03:32 PM (19 years, 4 months ago)

Are the syringes and needles still sterile packaged?
If yes you can just use them without pressure cooking.
I always inoculate the bags on the open air and not in the glove box.
Just make sure that you insert the needle through a drop of alcohol and close the hole afterwards with some glue or tape.
If you reuse the needle I would sterilize it with a lighter.


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Syringes, Sterility question [Re: ragadinks]
    #2933765 - 07/27/04 06:02 PM (19 years, 4 months ago)

I just put the needle on in back of a flame -- two each his/her own.

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Offlinebent
bent1

Registered: 07/31/03
Posts: 204
Loc: ny
Last seen: 17 years, 23 days
Re: Syringes, Sterility question [Re: micro]
    #2936566 - 07/28/04 02:13 PM (19 years, 4 months ago)

i stabbed myself with the fucking syringe and my right hand pointer finger was injured so bad its out of commision for at least i weak. it feels like it was broken i stabbed myself about half an inch deep into my finger right by my knuckle. other than that everything went aok. lol stupid me


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Offlinemeanoldman
old guy
Registered: 06/14/04
Posts: 96
Last seen: 3 years, 6 months
Re: Syringes, Sterility question [Re: bent]
    #2937178 - 07/28/04 04:51 PM (19 years, 4 months ago)

Get blunt tipped needles, they sell them for dispensing stuff out of syringes. We have tons of them at work for dispensing flux and epoxies and such. They work with any Luer based syringes.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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