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Offlinesonichell
journeyman
Registered: 11/01/99
Posts: 47
Last seen: 20 years, 8 months
Re: agar cultures??
    #293023 - 04/14/01 11:54 PM (21 years, 5 months ago)

rapid expansion of mycellial mass = more innoculant = more jars. and the whole pure strain isolation thing, although this is a little bit time consuming and takes several transfers, im sure there are tricks to it but my friend is new at it. being able to clone a mushroom is another plus because that IS a pure strain automatically. 1petri can innoc 10 jars. 1 print could probably make god knows how many plates. get one pure strain plate and that could go to 10 or more other plates then those ten plates to 100+ jars, hell yeah.



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OfflineMNmyc
enthusiast

Registered: 02/26/01
Posts: 175
Loc: MN
Last seen: 15 years, 6 months
Re: agar cultures?? [Re: sonichell]
    #293091 - 04/15/01 01:23 AM (21 years, 5 months ago)

I have been having huge problems with mold since spring has arrived.

And I find that using agar tends not to produce the type of growth that you are referring to. I have to do several transfers to different media to get what you are looking for.



--------------------
Life is what happens while you're making other plans...


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Anonymous

Re: agar cultures?? [Re: MNmyc]
    #293158 - 04/15/01 03:24 AM (21 years, 5 months ago)

Agar culture + pure strain isolation + good sterile Technique= UNIFORMITY
Of course if you pick a bad strain out of these , well you can imagine. Or if the proper environment isn't maintained, you can imagine.
Uniformity=increased yield



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