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OfflineOOISI
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Registered: 03/22/04
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Loc: S.Australia
Last seen: 5 months, 17 days
confirmation
    #2914588 - 07/22/04 02:37 AM (12 years, 9 months ago)

ok, i was making prints

this is what i did

Put on latex gloves and disposable face mask , rubbed alcohol on gloves.
Cut alfoil, rubbed Isopropyl on the foil pieces than wiped with dry tissue.
Got sterile blade inserted into scalpel top, put iso on the blade and wiped with dry tissue. cut stem as high up as possible on each shroom, got small tupperware container rubbed inside with Iso , wiped clean with tissues.
Placed caps down on foil. Waited two days.

Put on gloves wiped with alco wearing face mask. Took off tupper got jbags (prev wiped with iso) picked up the prints folded them placed in jbag and sealed em.

when i took of the tupper ware the prints were exposed to air (the last one like 2 and a half min. So some had exposure ranging from 10 seconds - 3 min. is this bad?

did i do anything wrong? any tips?

cheers for any help


Edited by OOISI (07/22/04 08:13 AM)


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OfflineJaRRn
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Re: confirmation [Re: OOISI]
    #2914595 - 07/22/04 02:40 AM (12 years, 9 months ago)



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OfflineOOISI
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Re: confirmation [Re: JaRRn]
    #2914744 - 07/22/04 03:44 AM (12 years, 9 months ago)

i want confirmation if its sterile, ive already done the process
i dont have a PC or a GB, and i cant do what he done to get the caps mine are from the wild. i dont have those awesome jars either.

did i just waste my time doin my method? (non viable prints)
the time of exposure was very bad! wasnt it

it helps, esp if i get a pc

any reported ways of doing it without a pc or gb and it making sterile prints?


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InvisibleMagashM
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Re: confirmation [Re: OOISI]
    #2914762 - 07/22/04 03:59 AM (12 years, 9 months ago)

You only need to set the cap on the foil for one day and you will have a dark print. What you want to do os put it on another piece of foil. Very easy to get 2 prints out of each cap.

Be sure to let the prints dry for a day.

Just replace the pc part with the alch wipe

Well this is how I like to do it. I like to get my foil ready like this That way when the print is done you have these flaps to fold over.

I then get a half pint jar (wide mouth) and prepare it like this. I ditch the disk and silicone in a tyvek disk. This will allow the cap to dry a little as sits in the jar. (Which causes more spores to drop and prevents the cap from rotting. Will also allow the prints to dry once the mushroom cap is removed.)

Next put in the foil and cover with the lid. Put some foil on top and pc for one hour at 15 psi.

Now uncover and wipe off and your little print chamber is ready.

Now take the jar to your mushroom chamber and loosen the top but don?t remove it.

I like to put a pin in each of the mushroom caps I?m going to print.

Cut the shroom just below the cap and put it on the foil in the jar as fast as you can. Easy to do since the jar lid is loose.

Put it in a glovebox or where you like to do your work.

In 24 hrs open up and take out the cap by the pin (now we know what the pin is for) and put the cap back on. Let it sit for 24 hrs again to let the spores dry.

Once dry fold up foil and store in a cool dry place


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Invisible@cro
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Re: confirmation [Re: OOISI]
    #2914772 - 07/22/04 04:07 AM (12 years, 9 months ago)

Your taking prints of wild specimens... I don't think anyone could/should expect them to be clean, you sound like you took the time to eliminate the sources you could, that's all anyone can expect from wild prints.
Plus they're wood lovers, they'll start on cardboard pretty easily without too much concern of contams.
Peace


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OfflineOOISI
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Re: confirmation [Re: @cro]
    #2914894 - 07/22/04 06:03 AM (12 years, 9 months ago)

thanks for the help all


Edited by OOISI (07/22/04 08:11 AM)


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Invisiblecricket
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Registered: 08/29/03
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Re: confirmation [Re: OOISI]
    #2915097 - 07/22/04 08:57 AM (12 years, 9 months ago)

You did good.


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I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?


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