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OfflineoOjonahOo
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Registered: 02/04/00
Posts: 345
Last seen: 15 years, 11 months
Re: The Great Azure challenge.. [Re: Kevin]
    #299479 - 04/23/01 12:47 PM (21 years, 5 months ago)

Go for it....professors eat this stuff up ;)

Your technique sounds great, lets hope it works as planned.
I think its a good idea spawning the woodchips before hand...the thought of all the grain out there doesn't sound to good in regards to pests...

Is birdseed the ideal spawning substrate for this? well maybe not ideal, but does it work well? I think maybe pure grain may work better, sometimes birdseed can be a bit unpredictable. Does stamets have anything to say on this? They are similar to psi. cyan. right? what does he say for those?

I emptied my bladder
at the top of a tower
and pee'd on the earth below...
...Fuck You, World!


--------------------
Ultimate Mycodirectory v4.0


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OfflineKevin
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Registered: 06/04/00
Posts: 676
Loc: Oregon
Last seen: 12 years, 2 months
Re: The Great Azure challenge..
    #299684 - 04/23/01 09:57 PM (21 years, 5 months ago)

Yea, a syringe should work fine. I'd use a syringe if I had one, but only have a print. Agar is good if you are using prints, to get a clean piece of agar to transfer, or to isolate a fruiting strain from a mushroom.

"I ask a mirror... "am I....god?", a mirrior replies. "God..... I am!"" -- Mitchnast


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"Is it a mile walking, or a mile driving?" - dobie



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Offlinefirstaid
journeyman
Registered: 04/23/01
Posts: 56
Last seen: 21 years, 3 months
Re: The Great Azure challenge..
    #300839 - 04/25/01 03:18 AM (21 years, 5 months ago)

If ya want everyone can send their results to me for proper testing!



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OfflineSuntzu
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Registered: 10/15/99
Posts: 1,396
Last seen: 3 months, 22 days
Re: The Great Azure challenge.. [Re: firstaid]
    #301633 - 04/25/01 09:42 PM (21 years, 5 months ago)

Just got back from Seattle; set up a couple very nice cyanescens and azurescens beds. They are within 15 feet of each other, and I plan to stick a bohemica bed nearby as well.
Specs: All three cultures were CLONES :) obtained from a massively cool Shroomerite/Drooler. After a purity passage to PDA, they were each blended using a home'made' eberbach [sterilized water ~1/2 full in 3 1/2 pt jars, add a few sections of agar, screw blender base onto jar, BZZZZZZ. . . liquid inoculum]. This inoculum was poured [about 5 mL's or so] into qt BS jars. Incubating at 80, colonization complete within a week.
Alder: Little Chief brand, simmered in molasses water [10 mL molasses per gallon tap water, ala Stamets] then let the chips cool/'ferment' for about 3 days. These were then cooked at 15 PSI 1.5-2.0 hours.
The trays: alternating layers of 1/2" alder, a sprinkling of grain, forming a sandwich about 4" thick.

I would have liked to made one more expansion onto straight woodchips [dilute the grain] but didn't have time. So I spawned these biscuits [which colonized VERY quickly, may have used too much grain] into my special Seattle spot using woodchips I borrowed from a local park [they have MOUNDS, perfect size variations, non-cedar].

My biggest fear is rats, but we'll see. Each of these spots has just a bit of grass/flowers coming up here and there.
I hope it's a winner!




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OfflineAgarical
journeyman

Registered: 01/15/01
Posts: 21
Last seen: 20 years, 7 months
Re: The Great Azure challenge.. [Re: Suntzu]
    #306190 - 05/01/01 05:25 AM (21 years, 5 months ago)

Suntzu, i'm gonna try your liquid innoculation tech onto peroxidated wood pellet fuel and use this to spawn fresh oak chips, what do you think? Whay can't you just use the liquid innoculant to innoculate pure wood chips?




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OfflineSuntzu
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Registered: 10/15/99
Posts: 1,396
Last seen: 3 months, 22 days
Re: The Great Azure challenge.. [Re: Agarical]
    #306294 - 05/01/01 07:13 AM (21 years, 5 months ago)

Hey Agarical, don't let me stop you but my experience has been that it doesn't work so well. Maybe the mycelium needs the grain for a nitrogen boost as it becomes acclamated to the wood, I really don't know.
I tried this with bohemica; all the areas that had a visible agar chunk [yeah, agar-wood transfers seem like they'd contaminate, but never has happened so far] showed some nice fuzzy recovery. Nearby chips would get a thin wispy growth, nothing I was excited about. It never really got further than this, I ended up spiking that tray with bohemica grain.
On the other hand, colonized grain jumps off to woodchips very nicely and quickly. I made a couple more of my azurescens/cyanescens sandwiches, they rebound and begin chip colonization within a couple days. This 'super spawn' [high grain content] works well to AT LEAST a three-fold expansion to larger woodchips [maybe more].
I've tried the direct liquid-woodchips route with Reishi; it worked, but it was so slow to recover I won't do that again. Stamets has that grain step in there for a good reason, it makes a big difference in both the vigor and speed of wood colonization.

BTW, the coir mixed in 1:2 with the woodchips is working fabulously for all woodlovers I've tried. The moisture of my trays looks much more uniform, mycelium from both azure and cyan is reaching deep into coir territory. Coir is the greatest thing I've come across since birdseed. So versatile, clean, perfect water holding capacity. Can't say enough.



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OfflineAgarical
journeyman

Registered: 01/15/01
Posts: 21
Last seen: 20 years, 7 months
Re: The Great Azure challenge.. [Re: Suntzu]
    #315239 - 05/12/01 06:25 AM (21 years, 4 months ago)

thanks Suntzu, i think i'm gonna try the azures on millet first to be on the safe side. I will also try the liquid innoculation too. I'll post the results soon. What brand of coir do you use?



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