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Invisiblesolidstate
journeyman
Registered: 04/09/00
Posts: 56
Re: CROSSBREEDING CONTEST [Re: nononsense]
    #329550 - 05/30/01 06:37 PM (21 years, 4 months ago)

yep, but you will still need a fair number of matings to be sure of getting a good fruiting strain...the above is the not fucking around here, take no prisoners, hybrid strain to end all hybrid strains approach...obviously it could be scaled down some for more modest aspirations, but youre still gonna need a microscope & lots of petris



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OfflineDarK_SavioR
addict
Registered: 05/06/01
Posts: 454
Loc: Down the Street
Last seen: 20 years, 5 months
Re: CROSSBREEDING CONTEST
    #329602 - 05/30/01 08:22 PM (21 years, 4 months ago)

hehe, nevermind... I'm wrong about the phantasia mystery strain ; ) hehe, oh well... good luck

"Now chew em up and slam the orange juice. Vitamin C chase, kill the taste. You can tell its nasty by the look on my face."

Long Live The Free Spore Ring!


--------------------
Vitamin C chase, kill the taste. You can tell its nasty by the look on my face.
Ralphster44 & The FSR!
All thats stated above is for humor and a lie!!


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Anonymous

Re: CROSSBREEDING CONTEST [Re: solidstate]
    #330105 - 05/31/01 05:27 AM (21 years, 4 months ago)

Solidstate gets an A in mushroom breeding, everyone else gets a C- and worse. Good post Solidstate. This is ecxactly how one would determine a mushrooms reproductive scheme. It is also how one would develope hybrids between spore races, and also how one would develope a single strain that has specific qualities. Doubt anyone will attempt, but damn good post.



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OfflineaNaPhylaktik
journeyman
Registered: 05/02/01
Posts: 40
Loc: Western Australia
Last seen: 20 years, 5 months
Re: CROSSBREEDING CONTEST [Re: mycofile]
    #330243 - 05/31/01 08:29 AM (21 years, 4 months ago)

see trademlets hybridistauion tek at Mystical mycology australia
www.shaman-australis.com

whats so frikking hard about serial dilutions anyway?
there are definitely reasons for hybridising strains such as

potency
substrate adaptability (lignous cubes and B+ with its ability to eat anything)
in vitro pinning (matias romero)
fast growth (cambos)
size (aussie and indian cubes)

i intend to try it myself. all you are going to need is lots of petri dishes and sterile transfer areas.
you would be attempting to create a new spore race - that is a number of confirmed F1 hybrids between
the strains that are then grown for successive generation allowing emergence of a new strain.you can select out for 2 chracteristics one from each parent eg in vitro pinning (MR) and Substrate adapt (B+)
fairly simple id say
with a good labelling system you could link the traits to specific monokaryons which gives you the ability to
create real strains
see books by chang et al and stamets and chilton as well as the trade omlet tek

Want to trade? edibles and psychoactives, spores and slants.


--------------------
visit the Australasian free seed ring
www.shaman-australis.com.au/seedring


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OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,396
Last seen: 3 months, 25 days
Re: CROSSBREEDING CONTEST [Re: Bleuboxo]
    #330654 - 05/31/01 09:09 PM (21 years, 4 months ago)

For some reason I see a 96 well plate coming in handy here.

E.g. using watchmaker-type tweezers [or a robot] to pick out the monokaryons, put two in each little well.

The resulting opacity/speed of growth should give you a minimal idea of vigor, then maybe you can reisolate and fruit only the best wells.
This might save on agar plates and working area, you could use a minimal medium [low contam potential] in your 96 well plate.

Man, what a pain, no matter how you look at it. It's possible, though.

I only eat veils


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OfflineaNaPhylaktik
journeyman
Registered: 05/02/01
Posts: 40
Loc: Western Australia
Last seen: 20 years, 5 months
Re: CROSSBREEDING CONTEST [Re: Suntzu]
    #331315 - 06/01/01 11:44 AM (21 years, 4 months ago)

1 points worth repeating that have been mad in literature is that vigour in monokaryons doesnt mean much so selecting for that can be detrimental
im trying to innoculate a local vet paddock with shrooms many strains hopefully these and hybrids of thes wil then be spread around the district as most herds would have one or more of their members go through it :)

Want to trade? edibles and psychoactives, spores and slants.


--------------------
visit the Australasian free seed ring
www.shaman-australis.com.au/seedring


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OfflineTriple_3
member
Registered: 03/22/01
Posts: 99
Last seen: 21 years, 3 months
Re: CROSSBREEDING CONTEST
    #331346 - 06/01/01 01:11 PM (21 years, 4 months ago)
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and this


isolate single spores-

Edited by Triple_3 on 06/01/01 06:17 AM.



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OfflineTriple_3
member
Registered: 03/22/01
Posts: 99
Last seen: 21 years, 3 months
Re: CROSSBREEDING CONTEST [Re: Triple_3]
    #331348 - 06/01/01 01:14 PM (21 years, 4 months ago)
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need a microscope... =)

agree with mycophile read TMC


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