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Amazon Shop: ½ Pint Jars, 10cc Syringe

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OfflineKMK
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Registered: 06/29/04
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Cool down time for jars - I screwed up?
    #2907807 - 07/20/04 10:54 AM (12 years, 10 months ago)

I was reading another thread about cool-down time for jars after you steam/PCook them..

when i did, i turned off the heat, had the kitchen fan blowing and the stove exhaust on..let sit for 20, they were cool to the touch..took them to my room and had a rotating fan blow on them for another 10. they were cool to the touch, so i inoculated.

now, i think i probably killed the spores (i hope not). because i'm guessing the substrate in the middle still wasn't cool, and probably killed the spores..

am i wrong? will i still be ok? i'm on day 5, no sign of anything but substrate yet..


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OfflineRecycledBodies
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Registered: 07/19/04
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Re: Cool down time for jars - I screwed up? [Re: KMK]
    #2907829 - 07/20/04 11:01 AM (12 years, 10 months ago)

give it more time, my cousing didnt wait to long after sterelizing them and his grew still


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InvisibleShroomismM
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Re: Cool down time for jars - I screwed up? [Re: KMK]
    #2907849 - 07/20/04 11:08 AM (12 years, 10 months ago)

errr... cool to the touch does not mean that the substrate is cool in the middle.. 30 minutes is probably not enough time for them to cool completely, chances are you nuked the spores. I usually let them cool for at least a few hours, if not overnight.. just leave them in the PC after you cook them.

I've killed spores by innoculating almost an hour after PCing.. they were cool to the touch and I let them sit for another half hour.. still no good. As a rule of thumb I just wait for at least 2-3 hours, and if you are really impatient stick them in the freezer for little bit.

Unless you are doing whole grains.. which cools off much faster because you can shake the grains.. but still give it an hour or two to be safe.


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OfflineKMK
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Re: Cool down time for jars - I screwed up? [Re: Shroomism]
    #2908082 - 07/20/04 12:34 PM (12 years, 9 months ago)

ah shit. i guess this means i gotta start over again..i should probably just leave these go for another couple days..no signs, i'll toss them.

i now need spores..i got mine from sporebank.com..any other suggestions? theyre too expensive.


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OfflineDr. Greenthumb
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Re: Cool down time for jars - I screwed up? [Re: KMK]
    #2908098 - 07/20/04 12:41 PM (12 years, 9 months ago)

Why not just re-innoc them if no growth shows in a week or so? Should still be sterile.


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OfflineKMK
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Re: Cool down time for jars - I screwed up? [Re: Dr. Greenthumb]
    #2908143 - 07/20/04 12:59 PM (12 years, 9 months ago)

i guess i could try that? what i might do is try that one website that offers 4 syringes for 26$. use 1 syringe on re-inoc them, then the others with another batch.

you sure it's safe to do that?


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InvisibleShroomismM
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Re: Cool down time for jars - I screwed up? [Re: KMK]
    #2908400 - 07/20/04 02:21 PM (12 years, 9 months ago)

as long as you didn't inject too much spore solution.. making the substrate too wet.. shouldn't be a problem.


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OfflineKMK
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Re: Cool down time for jars - I screwed up? [Re: Shroomism]
    #2908412 - 07/20/04 02:24 PM (12 years, 9 months ago)

i used a 10cc (11 or 12 cc with air) syringe for 6 cakes..


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InvisibleShroomismM
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Re: Cool down time for jars - I screwed up? [Re: KMK]
    #2908420 - 07/20/04 02:26 PM (12 years, 9 months ago)

it's worth a try.. if you don't see any growth within a couple days.


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Amazon Shop: ½ Pint Jars, 10cc Syringe

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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