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Tweeq
Tweeq of Nature


Registered: 06/07/18
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Re: The Official Woodlovers Thread [Re: cybesa] 3
#29071120 - 12/29/24 09:10 AM (17 days, 18 hours ago) |
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Quote:
cybesa said: I had totally no idea! Thanks for telling me, you probably saved me some issues down the line. I've since then, I have been able to obtain a Flow Hood and its where I do all the work now, so I suppose I could take the spawn bags with freshly soaked chips inside (after dripping as much as possible) and place them in front of the hood for several hours (or maybe days) to dry depending on their conditions. 
I'm not sure if it would be better to Autoclave the bags right when they are still kinda wet, or after these several hours to few days of drying in front of the hood has taken place? I'm thinking its easier to sterilize the wood while its still wet, and then put them in front of the hood to dry after sterilizing? 
So what they said, basically. Since you've had 2 responses already..
No I would recommend to dry thrm first and only load into bags when the moisture is right.. It makes no sense to sterilize any substrate way too wet and then open the bag up to dry in flow.
This is kinda why we told you to build a sab first. To get an understanding of what you're doing before buying expensive and vulnerable kit. A flowhood is not a drying device nor a magical tool that will make everything go right.
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cybesa
Loving soul



Registered: 05/24/18
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Loc: Europe
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Re: The Official Woodlovers Thread [Re: Mr Piggy]
#29071495 - 12/29/24 03:08 PM (17 days, 13 hours ago) |
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I guess I was misunderstood, of course I would never take the actual Chips out of the bags post PC, that's just silly!  I meant just put the actual woodchips bags in front of a hood or any air flow to dry - the air should penetrate through the breathing port to allow for drying is my theory, although I am not sure how effective that is..
So basically I was wondering if you can dry the chips in their final substrate bag pre-cycle, then when they are done, just sterilize them and they are ready to go!
-------------------- Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.
Edited by cybesa (12/29/24 03:16 PM)
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cybesa
Loving soul



Registered: 05/24/18
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Loc: Europe
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Re: The Official Woodlovers Thread [Re: Tweeq]
#29071501 - 12/29/24 03:13 PM (17 days, 12 hours ago) |
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So dry them first thing post soaking and when they are the right moisture, load into bags and then put them in the PC/Autoclave.. gotcha.
Quote:
This is kinda why we told you to build a sab first. To get an understanding of what you're doing before buying expensive and vulnerable kit. A flowhood is not a drying device nor a magical tool that will make everything go right.
Of course, I am aware. Sterile technique is everything, a flow hood just helps with better access/less back pain/better visibility. Overall just a better work flow 
If you were to grab a bag post-cycle and then open it in front of a flow hood for several hours (of course there were experiments where open plates were left In front of a proper hood and they were fine) you are asking for trouble. If you go ahead right into your workspace after gardening with a couple inches of dirt on your fingers while also sneezing into your bag, a flow hood isn't gonna save you.
-------------------- Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.
Edited by cybesa (12/29/24 03:17 PM)
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Blopblop
F-Tard


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Re: The Official Woodlovers Thread [Re: cybesa]
#29071659 - 12/29/24 06:08 PM (17 days, 10 hours ago) |
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Hey Cybesa, here is how I deal with properly hydrating woodchips. First if they are bone dry I’ll soak for a few hours. The next step is to boil them. The idea here is to get them as hot as possible. After that, drain them into a strainer and let them steam dry on sheet pans. Hours later the chips will no longer feel wet but will still have moisture in them, if that makes sense. Very much like grain prep. Even though they might seem overly dry, after pcing you will see moisture in the bags but without any pooling. I usually let them dry overnight before bagging.
Edited by Blopblop (12/29/24 06:16 PM)
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Nichrome
Participle Phrase



Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: The Official Woodlovers Thread [Re: cybesa]
#29071662 - 12/29/24 06:10 PM (17 days, 9 hours ago) |
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You don't get much evap from GE ports. You want to lay all your hydrated chips out in a layer till they look dry on the surface, then bag/jar and PC. Sheet pans or something of the like. It doesn't need to be fancy.
After the PC they appear moist again.
-------------------- discussions are a healthy alternative to arguments
There is only one electron, and it's you.
Edited by Nichrome (12/29/24 06:11 PM)
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Scemilope
Jar Washing Enthusiast


Registered: 11/16/22
Posts: 1,428
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Re: The Official Woodlovers Thread [Re: Nichrome]
#29071788 - 12/29/24 08:38 PM (17 days, 7 hours ago) |
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-------------------- This hobby has taught me many things.
Most of those things are patience.
The Hitchhiker's Guide to the Shroomery
LAGM 2025
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cybesa
Loving soul



Registered: 05/24/18
Posts: 251
Loc: Europe
Last seen: 11 hours, 57 minutes
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Re: The Official Woodlovers Thread [Re: Blopblop]
#29072093 - 12/30/24 08:12 AM (16 days, 19 hours ago) |
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That sounds good! The fact that they will be more moist post cycle is really reassuring.. 
Thanks guys!  Gonna report back on how it went once I get some woodlover activity!
-------------------- Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.
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Churpee Choozle
Towel



Registered: 11/11/24
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Re: The Official Woodlovers Thread [Re: cybesa] 2
#29072112 - 12/30/24 08:43 AM (16 days, 19 hours ago) |
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Yoshi's post in the IWL thread really got me thinking... (The agar to woodchips pic)
With IWL, I want to run to the finish. Pretty much the same with ovoideocystidiata. I need large amounts of settled mycelium before April. But with subaeruginosa... More than enough time. So as a shits and giggles project I dropped a wedge in a jar of sterilized Oak / Beech chips. It's taking off nicely. Also tested the old ovoid LC on agar (after reading so many stories on dirty LC, I actually wondered if I hadn't been producing crap the past years. Even linked some post-spawning contams to my LC...). Came out nice and clean. So tonight I'll inoculate some plain woodchips with LC. No reinventing the wheel here. Just want to see if it's possible to skip grains, what it'll do in terms of colonization time, toughness of spawn etc. Guess it has been done before a million times, but curiosity eh. My outdoor beds suffer first and foremost from animals that are after grains (and later on grubs and other larvae).
My crappy photography skills don't do it justice, but I'll update when it gets serious.
-------------------- "Shoot! Fuck! Scag! Blood! Heroin! Rape! Cheap! Communist! Jab it right into your fucking eyeballs!"
- Hunter S. Thompson
Touching your first grows unnecessarily is very similar to crack.
- Alaskappalachian
Things to consider when you (re-) start growing mushrooms. https://www.shroomery.org/forums/showflat.php/Number/28501530/fpart/1/vc/1
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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 3,109
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 Wavzzies pinning and the fruit pushing from same neglected jar fruiting last winter. Just 60f basement temps and completely neglected with lid off no joke
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: The Official Woodlovers Thread [Re: rhizoRider]
#29072803 - 12/30/24 09:43 PM (16 days, 6 hours ago) |
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I’ve got a dumb question for you.
Why is it woodlovers can even colonize grain?
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EniQma
Shroomery Addict

Registered: 11/28/23
Posts: 1,858
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Re: The Official Woodlovers Thread [Re: Smellyhobbit]
#29072829 - 12/30/24 09:56 PM (16 days, 6 hours ago) |
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I’ll answer your question with a question. What can wood lovers not colonize?
-------------------- Do what you need to when you have to so you can do what you want to when you can.
-Me
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Smellyhobbit
Actual Retard



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Re: The Official Woodlovers Thread [Re: EniQma]
#29072833 - 12/30/24 10:00 PM (16 days, 6 hours ago) |
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Steel.
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Churpee Choozle
Towel



Registered: 11/11/24
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Loc: NW Europe
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Re: The Official Woodlovers Thread [Re: Smellyhobbit]
#29072929 - 12/31/24 02:37 AM (16 days, 1 hour ago) |
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Seen them eat glass. But I don't get the question. Is there a punchline? Or is this more of the lignicolous / cellulosic / activate your set of enzymes here kind?
-------------------- "Shoot! Fuck! Scag! Blood! Heroin! Rape! Cheap! Communist! Jab it right into your fucking eyeballs!"
- Hunter S. Thompson
Touching your first grows unnecessarily is very similar to crack.
- Alaskappalachian
Things to consider when you (re-) start growing mushrooms. https://www.shroomery.org/forums/showflat.php/Number/28501530/fpart/1/vc/1
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Churpee Choozle
Towel



Registered: 11/11/24
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Loc: NW Europe
Last seen: 19 minutes, 21 seconds
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OK it is my job to tell people about woodrotting fungi (and kill the hosts of said fungi, in most cases), so I always hesitate when people ask a really general question, give me specifics. I think there's a hook at the end of your question, but for those who might be like 'yeah why is that even'...
Saprophytic fungi eat carbohydrates. They can all, like all plants and animals, do their thing with most sugars, 'cause those are short-chain, easy to digest carbohydrates. Most / all of the fungi we grow eat cellulose, the longer-chain carbohydrate that is at the heart of every plant. Cellulose is a longer chain carb, harder to break down. Really long chains, like fuels or plastics, are often simply indigestible under 'natural' circumstances . All of the Psilocybe woodlovers (Gyms are a different story) we grow eat lignin, which together with cellulose and hemicellulose is the building blocks of wood. Lignin is a long chain, real motherfucker to digest and requires drastic measures (ligninases, the lignin peroxidases Nyx was so crazy about), that only our woodlovers can take. Mushrooms have different sets of genes for the production of different sets of enzymes. They can adapt to a wide range of foodstuffs. Our woodlovers can easily adapt to the cellulose and other carbohydrates grains are made of. They can adapt to sugars in a LC and be just fine. As long as there's C in there. They just don't seem to fruit off it. Most Gyms are brown rotters / cellulose eaters and should be able to fruit off substrates that are devoid of lignin.
I tried my best not to come up with a jargon-heavy thing, but had to round so many corners in doing so that I'm afraid I oversimplified it. Please correct me if I'm wrong.
-------------------- "Shoot! Fuck! Scag! Blood! Heroin! Rape! Cheap! Communist! Jab it right into your fucking eyeballs!"
- Hunter S. Thompson
Touching your first grows unnecessarily is very similar to crack.
- Alaskappalachian
Things to consider when you (re-) start growing mushrooms. https://www.shroomery.org/forums/showflat.php/Number/28501530/fpart/1/vc/1
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cybesa
Loving soul



Registered: 05/24/18
Posts: 251
Loc: Europe
Last seen: 11 hours, 57 minutes
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I appreciate you taking the time to leave such educational information here! I love to see enthusiasm. These are fascinating creatures. 
Now I have a proposal: I am a fun-gi, so I've obtained comically oversized, 180mm x 30mm and 150mm x 25mm petri dishes. The question is: Should I use these normally? I could do multiple different cultures on each due to their immense size, like 6 dividing the dish into 6 different sectors. I suppose there is no harm using it, the only issue is the higher chance of contamination due to larger surface area, so I'll try to remedy that by leaving it open even less and open partially while innoculating! I'll report back how it went!
-------------------- Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.
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NothingsChanged
Striving for Excellence


Registered: 05/28/11
Posts: 10,674
Loc: North/Western WA
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Re: The Official Woodlovers Thread [Re: cybesa]
#29073183 - 12/31/24 10:32 AM (15 days, 17 hours ago) |
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When your culture is clean you will have a giant agar puck to inoculate with.
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cybesa
Loving soul



Registered: 05/24/18
Posts: 251
Loc: Europe
Last seen: 11 hours, 57 minutes
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Gonna be fun looking once I get pictures. I'll cycle them for 35 mins once at pressure instead of 15-20 as is procedure for regular petris, just to be safe!
-------------------- Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.
Edited by cybesa (12/31/24 11:27 AM)
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Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,916
Loc: WA
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Re: The Official Woodlovers Thread [Re: cybesa] 5
#29073762 - 12/31/24 08:39 PM (15 days, 7 hours ago) |
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Some cyan and azure master jars coming along nicely. Clone, maybe isolate, cultures from a website I am not necessarily super stoked on - but hoping it's gonna perform decently. Gonna expand a bit and then just spawn these in February in some spots around Seattle.
Y'all think they'll be fine to colonize chips outside if spawned before Spring? I'm waiting until mid-march/April before spawning beds at my home (following liloldme's approach basically) but I want to get the process started spreading the myc around the city as soon as I can.
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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
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Banfir
Stranger
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Re: The Official Woodlovers Thread [Re: Psicomb] 7
#29073768 - 12/31/24 09:12 PM (15 days, 6 hours ago) |
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Have been having a blast on this site learning, so thought I’d leave my latest Azure project photos, taken earlier today. The jars contain the myc two transfers from the plate with the pin.



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NothingsChanged
Striving for Excellence


Registered: 05/28/11
Posts: 10,674
Loc: North/Western WA
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Re: The Official Woodlovers Thread [Re: Banfir] 1
#29073804 - 12/31/24 10:53 PM (15 days, 5 hours ago) |
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Sweet dude.
Psi, do it, it’s getting ready to be winter, doesn’t typically get to bad. If it does get cold and slow, in my “Opinion”, long as your grain is fully coloninzed. *whats up with he vendor? I got you.
Happy new year. Yea i am pretty boring these days.
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