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Offlinedanma
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Registered: 07/19/04
Posts: 31
Last seen: 12 years, 5 months
Question - Need tape in PC?
    #2906764 - 07/19/04 11:28 PM (12 years, 7 months ago)

Can you sterilize jars in the PC without any tape? I used tinfoil over the top, and used tape to kind of fasten the tinfoil on a little, but did not tape up the holes. And now its been 4 days without any sign of growth, despite following pf to a T otherwise. Just a little worried that might have introduced too much water into the substrate. Any comments or help?


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Offlineiamgod
just some dudesome where

Registered: 06/30/03
Posts: 263
Loc: Crooklyn, New York
Last seen: 8 years, 11 months
Re: Question - Need tape in PC? [Re: danma]
    #2906795 - 07/19/04 11:45 PM (12 years, 7 months ago)

You don't need tape, and I have personally never used tape with foil. Are you covering the jars with water in the PC? The water level should only be about an inch, not enough to come into the jars or to cover them.

Did you make sure your jars were cool before innoculating them? Were the syringes prepared correctly?

Give it a few more days, sometimes it took over a week for visible growth for me.


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OfflineJaRRn
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Registered: 05/21/04
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Re: Question - Need tape in PC? [Re: danma]
    #2906882 - 07/20/04 12:27 AM (12 years, 7 months ago)

I got 1 layer foil with 4 holes then pc'd with 1 foil layer over top, they r in the Tub in Tub with the top layer of foil removed. 10 days and kicking ass. no contams. Syringe used was crystal clear (not 1 sign of spores).


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