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OfflineTweexican
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Micron Magick - Myco Starter Injection lids
    #2895930 - 07/16/04 04:17 AM (12 years, 4 months ago)

I have had little success with liquid culture. I am wondering if maybe it might be due to my jar setups, so I have decided to try Micron Magick's Myco Starter Injection lids with the self-healing injection site.

I am wondering if anyone here has had any experience with them. My only concern is that there is no air exchange with these lids. Any comments would be appreciated.


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Invisiblemuse_sick
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Re: Micron Magick - Myco Starter Injection lids [Re: Tweexican]
    #2895943 - 07/16/04 04:24 AM (12 years, 4 months ago)

what problem are you having with liquid culture?
the lids are pretty much idiot proof
although with some cunning and access to the right materials you can make one your self


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InvisibleLoki
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Re: Micron Magick - Myco Starter Injection lids [Re: Tweexican]
    #2895989 - 07/16/04 05:01 AM (12 years, 4 months ago)

air exchang isnt so needed with liquid culture, as water contains a fair amount of o2. Have you tried adding a drip of h2o2 after the spores germinate in the water? This will also add extra o2.

Self sealing injection ports are easerly made using silicon gel blobs, i use fish tank sealing gel,as its a bit better than the normal batheroom sealing gel.


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InvisibleLoki
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Re: Micron Magick - Myco Starter Injection lids [Re: Tweexican]
    #2895993 - 07/16/04 05:02 AM (12 years, 4 months ago)

p.s dont use dh2o as it has no o2 in, use bottled spring water instead.


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OfflineTweexican
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Re: Micron Magick - Myco Starter Injection lids [Re: Loki]
    #2896587 - 07/16/04 12:26 PM (12 years, 4 months ago)

Well, I successfully grew a clump of mycellium in a karo solution. It was a little opaque and not really established, but I have heard you should harvest it right away. I squirted some peroxide and sucked it up into a sterile syringe. The jars that were innoculated have yet to show any signs of contam or colonization and its already been a week and a half. I think I may have harvested a little early... any ideas?


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InvisibleLoki
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Re: Micron Magick - Myco Starter Injection lids [Re: Tweexican]
    #2896596 - 07/16/04 12:29 PM (12 years, 4 months ago)

How much h2o2 did you use? As you need a tiny amount.
you might have shocked the mycelium.
Either that, or there is something wrong with the incubator temps, as
you should certainly see some growth by 10 days, I would hope to see signs of life by day 5 myself.


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OfflineTweexican
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Re: Micron Magick - Myco Starter Injection lids [Re: Loki]
    #2896615 - 07/16/04 12:38 PM (12 years, 4 months ago)

Loki, how long do you usually wait until harvesting the liquid myc?


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