Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Offlineeggmaneggman
Stranger
Registered: 08/05/24
Posts: 1
Last seen: 5 months, 8 days
Contam Syringes?
    #28901441 - 08/05/24 07:15 PM (5 months, 9 days ago)

Hello,

I've been trying to get started with agar, and I'm having this recurring issues with all my inoculation attempts with A+ spore syringes. All of my plates look like the pictures; it looks like bacteria to me, but I saw a few posts that suggested it might be just water on the agar. I've had no trouble getting my king oyster liquid culture to inoculate on dishes, so I think it has something to do with the syringes.

The two pictures were inoculated with the same syringe on two different days, with two different batches of agar (one no-pour, one not). I got the same result from another A+ syringe from this seller about two months ago (a trusted Shroomery vendor who I have had no problems with in the past). I inoculated both with a single drop of syringe solution, the excess moisture is from my no-pour having insane amounts of condensation. The apparent contam appeared within 24 hours of inoculating.

I'm just looking for a sanity check here - this is probably an issue with the syringes, right? Maybe they were made in the same dirty batch, or from the same extra-dirty print? I figure I'll give them two weeks to see if any myc shows up to clean up with a few transfers, but my hopes aren't high.




Extras: Filter Print Post Top
OfflineSo Anyway
Boner donor


Registered: 07/11/21
Posts: 806
Last seen: 19 days, 11 hours
Re: Contam Syringes? [Re: eggmaneggman]
    #28901478 - 08/05/24 07:55 PM (5 months, 9 days ago)

Try germinating the syringes on BRF pucks, I've pulled clean cultures off some super bacterial old syringes that had been written off and about to be tossed.

Josex poke has more info, basically just make small pf tek pucks and drop some spores in. The poke aspect of that tek is a good idea to further separate from bacteria if you do get germination


--------------------
So anyway, uh...

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* All Contams! What Happened? trippingchef 1,216 17 02/27/05 02:00 AM
by HippieChick
* MycoBag Contam? (pics) PaintballAds 1,102 2 03/04/05 12:14 PM
by PaintballAds
* i've got B+ and EQ syrings NEED TO KNOW BEST SUBSTR8!! jaham 1,082 7 02/28/04 12:54 PM
by buckeye
* contam question Padidleniddle 435 1 04/11/04 03:33 PM
by Anonymous
* how do you tell if a syring is contaminated HarvestTheBrain 2,472 3 07/17/03 01:08 PM
by MAIA
* I am scared to do a Print/syring!
( 1 2 all )
fearforce7 9,321 27 12/30/21 08:54 AM
by Forrester
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 15,187 25 06/02/16 05:47 PM
by OldSawbonesHisself
* Is this why all my jars got contams? fearforce7 2,283 17 12/25/09 02:58 AM
by jokefox

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
91 topic views. 21 members, 102 guests and 93 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.006 seconds on 12 queries.