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OfflineHugh Jass
Stranger

Registered: 02/17/23
Posts: 1,052
Last seen: 12 hours, 54 minutes
Agar to grain...
    #28900217 - 08/04/24 05:13 PM (5 months, 10 days ago)

I have my plates growing nicely.
I run about six plates and slice them up into little pieces with my scalpel, then add half a plate to a jar, give it a shake and let nature take it's course.

Is this the best way?
Or should I just make one plate and then put the mycelium into a liquid culture mix and just let that grow out and put the LC into the jars when ready?


What is the best way?
Which one is fastest?

Or should I just carry on with the dishes if it works?

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InvisiblePhaethon
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Male

Registered: 07/04/22
Posts: 703
Loc: Colorado
Re: Agar to grain... [Re: Hugh Jass]
    #28900331 - 08/04/24 07:22 PM (5 months, 10 days ago)

Agar to grain jar is safer - the LC is a prime opportunity to grow bacteria if your technique is even a little lacking.

You can make a couple smaller mother jars (like pints) with a plate, which will colonize faster than quarts, and use one pint jar to inoculate like 5 quart jars (1:10 expansion) once it's fully colonized. That might be marginally faster than inoculating all the quarts at once since g2g is lightning fast by comparison, and definitely requires fewer plates.

Do what fits in your workflow. Maybe wait to do LC until you have consistent success with grain to grain though.

Edit: I should also mention LI. You can cut up the plate, add it to sterile water in a jar or media bottle, shake the shit out of it, and use it similar to how you would use LC. It's still probably riskier than straight agar to grain since bacteria thrive in water, but it's definitely giving bacteria less time to take over than LC.

Edited by Phaethon (08/04/24 07:28 PM)

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Offlineboxtrotalpha
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Registered: 06/17/24
Posts: 1,285
Loc: The rings of Saturn
Last seen: 4 months, 11 days
Re: Agar to grain... [Re: Phaethon]
    #28900348 - 08/04/24 07:41 PM (5 months, 10 days ago)

A lot of blenders will fit a regular mouthed mason jar right on it. Sterilize it full of water, dump a clean agar or 2 in it, blend it up and pour it right into your grain jars

https://www.shroomery.org/forums/showflat.php/Number/24015710#24015710

worth saying though, if agar to grain is working well for you it may be best to just stick with it and not risk screwing shit up

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OfflineBigdogg
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Registered: 06/30/24
Posts: 641
Last seen: 2 months, 29 days
Re: Agar to grain... [Re: boxtrotalpha]
    #28900356 - 08/04/24 07:49 PM (5 months, 10 days ago)

Agar to grain is the safest and the slowest. Make master jars as mentioned above and that will speed it up in the long run.

LC is the fastest but add a chance for contamination and food for the contamination.

LI is less risky and almost as fast as LC.

This is the easiest safest way to speed up the process. It's a mix of LI and LC.

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Offlinevicepope
The devils best grower
Male


Registered: 04/15/24
Posts: 4,995
Loc: The fear that burns in the hea...
Last seen: 11 hours, 46 minutes
Re: Agar to grain... [Re: Bigdogg]
    #28900364 - 08/04/24 08:07 PM (5 months, 10 days ago)

Slurry is the fastest.  Agar is based. Be based when in trouble.  Speed isn't important when for personal use. 2 weeks of speed is risky and costly. Li is great but runs into the sterile technique of pouring that you might as well be doing josex poke method.


--------------------
The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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OfflineHugh Jass
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Registered: 02/17/23
Posts: 1,052
Last seen: 12 hours, 54 minutes
Re: Agar to grain... [Re: vicepope]
    #28900561 - 08/05/24 02:03 AM (5 months, 9 days ago)

Thanks guys.

I appreciate the answers.
:airguitar:

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