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OfflineOrganic_Spirit
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Registered: 05/06/11
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Avoid The Toxic Transfer
    #28899729 - 08/04/24 10:43 AM (5 months, 10 days ago)

Hello family, i was curious what you all thought about different types of molds and making transferring isolated culture from other dishes.

Particularly slime molds. they dont seem to spread like other molds and are the spores even same?

I've posted a example.



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Offlinephenyl
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Re: Avoid The Toxic Transfer [Re: Organic_Spirit] * 1
    #28899786 - 08/04/24 11:51 AM (5 months, 10 days ago)

I'm not a fan of opening plates with sporulating molds on them in general. I throw the moldy plate, use a plate that isn't full of mold and save myself the trouble.

slime molds produce spores but they aren't mold, they're a funny informal clade of protists (which is in turn a funny informal clade of eukaryotes that we're still figuring out how to classify phylogenetically but that's for another time and person to discuss it.) that said, those plates look like they're carrying bacteria or yeast rather than slime mold and maybe some random household mold in the first pic. very few things you'll find growing on MEA/PDA in a household environment are of any real pathogenic potential. just don't lick a dish full of bacteria.


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The fool who persists in his folly will become wise.

Edited by phenyl (08/04/24 12:05 PM)

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OfflineB Traven
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Re: Avoid The Toxic Transfer [Re: phenyl]
    #28899849 - 08/04/24 12:40 PM (5 months, 10 days ago)

I don't lose sleep over it. I see it all as a numbers game.

My main objective is to get lots of plates going and use the cleanest ones. If I've got something truly special, last representative of its line, etc., then I'll mess around more with pulling transfers from plates with mold on them. If I've got cleaner specimens from that line, or it's just one of a bunch of germination plates, then I'll just toss it.

Between those two pictures, the top one with the yeast-looking spot appears more salvageable to me. Still plenty of clean growth opposite that spot. The bottom one has that weird fluff all around the growing edge, suggesting further contamination issues beyond just those slimy yellow spots.

Also, I'm gonna start making a declaration on posts like this: I use RE-USABLE VESSELS for my agar work. Pyrex petris and PP5 containers. I do not condone or encourage the use of disposable petri dishes, especially when making lots of different plates to test cultures and play the odds. Know that ANY time I mention a numbers game, running multiple plates and selecting the best one, etc., I am NOT suggesting that someone buy a bunch of disposable and/or pre-made plates in order to do that.


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Beware of advice- even this.

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OfflineOrganic_Spirit
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Registered: 05/06/11
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Re: Avoid The Toxic Transfer [Re: phenyl]
    #28899862 - 08/04/24 12:44 PM (5 months, 10 days ago)

Quote:

phenyl said:
I'm not a fan of opening plates with sporulating molds on them in general. I throw the moldy plate, use a plate that isn't full of mold and save myself the trouble.

slime molds produce spores but they aren't mold, they're a funny informal clade of protists (which is in turn a funny informal clade of eukaryotes that we're still figuring out how to classify phylogenetically but that's for another time and person to discuss it.) that said, those plates look like they're carrying bacteria or yeast rather than slime mold and maybe some random household mold in the first pic. very few things you'll find growing on MEA/PDA in a household environment are of any real pathogenic potential. just don't lick a dish full of bacteria.




What you said is interesting, i did not know that.
I was asking the question because out of the molds I've had slime molds seem to be different then other molds and thought if i were to try isolate and transfer plate to plate that slime mold might be one to worry less about?

As from a few plates i had isolated two demonstrated the type of growth i was looking for and one was contaminated with what looked to be slime mold.
other molds i would not touch and normally would toss all contaminated plates, but since i had two plates to work with, i was seeing if maybe anyone else did try to transfer from a slime mold contaminated plate and what success they may have had.


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OfflineOrganic_Spirit
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Registered: 05/06/11
Posts: 26
Last seen: 1 day, 5 hours
Re: Avoid The Toxic Transfer [Re: B Traven]
    #28899872 - 08/04/24 12:51 PM (5 months, 10 days ago)

Quote:

B Traven said:
I don't lose sleep over it. I see it all as a numbers game.

My main objective is to get lots of plates going and use the cleanest ones. If I've got something truly special, last representative of its line, etc., then I'll mess around more with pulling transfers from plates with mold on them. If I've got cleaner specimens from that line, or it's just one of a bunch of germination plates, then I'll just toss it.

Between those two pictures, the top one with the yeast-looking spot appears more salvageable to me. Still plenty of clean growth opposite that spot. The bottom one has that weird fluff all around the growing edge, suggesting further contamination issues beyond just those slimy yellow spots.

Also, I'm gonna start making a declaration on posts like this: I use RE-USABLE VESSELS for my agar work. Pyrex petris and PP5 containers. I do not condone or encourage the use of disposable petri dishes, especially when making lots of different plates to test cultures and play the odds. Know that ANY time I mention a numbers game, running multiple plates and selecting the best one, etc., I am NOT suggesting that someone buy a bunch of disposable and/or pre-made plates in order to do that.




Interesting i thought the bottom one looked more salvageable then the top one


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OfflineBigdogg
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Registered: 06/30/24
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Re: Avoid The Toxic Transfer [Re: Organic_Spirit]
    #28900124 - 08/04/24 03:57 PM (5 months, 10 days ago)

Quote:

Organic_Spirit said:
Hello family, i was curious what you all thought about different types of molds and making transferring isolated culture from other dishes.

Particularly slime molds. they dont seem to spread like other molds and are the spores even same?

I've posted a example.







The top one o would take 8-10 o'clock and 11 o'clock.

Bottom one I would take 1+2 o'clock.

Do just that in you SAB toss those plates after. Bleach everything.

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