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Offlinechfbrd
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Registered: 09/22/23
Posts: 117
Last seen: 18 hours, 6 minutes
Lc to LC ratio?
    #28899801 - 08/04/24 12:05 PM (5 months, 10 days ago)

Hello,
I am wondering how many ml of LC I should add to my LME/water mix to generate more LC?(LCtoLC).  for example is it 10ml to say 500ml?  or more like 100ml to 500 ml?  Just not sure how much of my good LC to make more?  I could do agar to LC but I am trying to get some LC without agar floating around in it to make it easier to suck up in syringe.
Thanks

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InvisibleCosmyc
Curious Psychonaut
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Registered: 01/26/22
Posts: 330
Re: Lc to LC ratio? [Re: chfbrd]
    #28899822 - 08/04/24 12:18 PM (5 months, 10 days ago)

If you don't plan to make lc with agar, what do you plan to start your mother culture with?

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OfflineRockinRobot
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Registered: 12/08/22
Posts: 1,893
Last seen: 5 hours, 45 minutes
Re: Lc to LC ratio? [Re: Cosmyc]
    #28899826 - 08/04/24 12:21 PM (5 months, 10 days ago)

Never trust vendor LC to make new. LC should be made from clean agar.

That being said it only takes a drop to inoculate fresh LC.

Personally I would grow it on agar and use Josex Poke to make your initial LC

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Offlinevicepope
The devils best grower
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Registered: 04/15/24
Posts: 4,995
Loc: The fear that burns in the hea...
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Re: Lc to LC ratio? [Re: RockinRobot]
    #28900201 - 08/04/24 04:59 PM (5 months, 10 days ago)

Yes always  test on agar first . To answer on the base of making your own lc as lc master to secondary lc. 2ml to 400ml of liquid culture media.  Never trust vendors,  sometimes it's not even there fault it was contaminated it's the post office fault.

Once you get lc masters and clean culture you can add 3 to 5ml of lc to 1 grain jar if you so choose.  Lc is powerful weapon .


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The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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OfflineSporeJunkie
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Registered: 11/30/18
Posts: 3,054
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Re: Lc to LC ratio? [Re: vicepope]
    #28900275 - 08/04/24 06:10 PM (5 months, 10 days ago)

Dude wth are you talking about with the post office line?


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I been drinkin green tea all goddamn day

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