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Offlineformidablemuffin
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Registered: 07/07/24
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LC Contam or Agar Remanents
    #28894264 - 07/30/24 09:29 PM (5 months, 15 days ago)

I dropped a wedge of agar into a jar that was made following the semi solid LC thread. There is a lot of myc in the jar, but a patch of something else. I was wondering if anyone has seen the agar wedge they dropped in change shape- I don't see it anymore, but there is a much thinner but wider wafer like piece of debrie. I'm wondering if there's any chance this debrie is the remanents of the agar wedge used to inoculate of if its definitely contamination.

[image][/image]

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OfflineCocaineBuffet
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Registered: 08/29/19
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Re: LC Contam or Agar Remanents [Re: formidablemuffin]
    #28894265 - 07/30/24 09:33 PM (5 months, 15 days ago)

If you don't test on agar you are wasting your time. What does that LC test plate look like?

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Offlineformidablemuffin
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Re: LC Contam or Agar Remanents [Re: CocaineBuffet]
    #28901491 - 08/05/24 08:15 PM (5 months, 9 days ago)

Thanks for the reply. I haven't tested it, but I've since read other threads that concurred with your advice. I don't think I'll waste a plate to test. I didn't add an injection port to this lid, I was planning on pouring it in the grain jars, so I'd be worried the LC would contam after opening the lid collecting a sample to test. I'm still fumbling in my SAB and learning to transfer agar smoothly.

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