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OfflineHeitentinger
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Registered: 08/20/17
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First time on agar. is there mycelium or only trichoderma ?
    #28888717 - 07/27/24 05:39 AM (5 months, 18 days ago)

Hello everyone.

I had quite a bit of experience inoculating spawn bags from spore syringe I was doing myself a few years ago. At the end, I could do it eyes closed. After a few years of break, I'm back doing it again but struggle to find a source of clean spore prints again. The ones I find seam dirty so I decided to purify them with agar after 3 fails inoculating grain spawns.

My first plate is growing lot of trichoderma (red circle) as I was expecting it as my spore print is full of it. but I was wondering if the white expanding things growing on the plate (green circle) was mycelium. doesn't look like it, but maybe some strain look like it on agar, so I had to ask.

Here is the picture of my try.





Plus, how can you isolate mycelium from the trichoderma when the trichoderma is growing faster than the spores.

I inoculated the spores straight to agar using an SAB and the procedure looks clean as my uninoculated petri dishes stayed clean for more than 2 weeks. My strain is cubensis ecuador.


Thank you all for your answers.

see you soon

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Offlinevicepope
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Re: First time on agar. is there mycelium or only trichoderma ? [Re: Heitentinger]
    #28888719 - 07/27/24 05:48 AM (5 months, 18 days ago)

It's bacteria and penicillin mold. Trash it.


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Offlinedr_harrybush
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Re: First time on agar. is there mycelium or only trichoderma ? [Re: vicepope]
    #28888732 - 07/27/24 06:20 AM (5 months, 18 days ago)

As mentioned above.

Bacteria + mold

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OfflineHeitentinger
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Re: First time on agar. is there mycelium or only trichoderma ? [Re: dr_harrybush]
    #28890128 - 07/28/24 02:15 AM (5 months, 17 days ago)

Ok, thank-you.

So I just have to do multiple trials until one petri dish lets appear a tiny bit of mycelium before I isolate further ?

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OfflineAlexandrDughin
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Re: First time on agar. is there mycelium or only trichoderma ? [Re: Heitentinger]
    #28890212 - 07/28/24 06:50 AM (5 months, 17 days ago)

Yes.
It is a number's game, inoculate a few plates with spores (5 or more ), then proceef from there, once the spores germinate.
Also : on my side of reality, anything that germinates in less than 3-4 days is contamination, so you might want to keep that in mind.

usually it takes 7-10 days for me to start seeing good micellium growing after spore inoculation.


( golden teachers )

Edited by AlexandrDughin (07/28/24 06:50 AM)

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