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OfflineMycCycle
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Wrapping plates and thoughts on agar.
    #28888688 - 07/27/24 04:54 AM (5 months, 18 days ago)

Hello fellow shroomerites,

Up until now I’ve been using grain water agar, but I figured I had to up the nutrition content.

I’ve now tried the following recipe:
Add 20gr homemade BRF (my grinder sucks, so it ain’t too fine) to my media bottle.
Add 4gr of Agar-Agar to the media bottle.
Fill the media bottle with water, to the 200ml mark.
Shake well.
Steam sterilise for 1h 15m.

I’ve inoculated the plates, but this time I haven’t wrapped them with anything considering I have finally improved my work space.

I have read that „I can get by without wrapping, but it’s not recommended”.

I guess my question is: Is it contamination that makes it necessary to wrap the plates, or is it moisture retention/safety? I am covering the SAB holes when the SAB is not in use.

All the best to you guys!

Here’s a little pic of the setup: (how do you like my new SAB? 😁)

Edited by MycCycle (07/27/24 05:12 AM)

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OfflinehamloafM
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Re: Wrapping plates and thoughts on agar. [Re: MycCycle]
    #28888710 - 07/27/24 05:22 AM (5 months, 18 days ago)

An hour and 15 minutes is a little long for agar sterilization.  You could back that time up at least a half an hour to 45 minutes, and save your self some time and energy.  We wrap plates as a barrier against contamination, not to reduce condensation.


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OfflineMycCycle
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Re: Wrapping plates and thoughts on agar. [Re: hamloaf] * 1
    #28888733 - 07/27/24 06:21 AM (5 months, 18 days ago)

Thank you Hamloaf, for straightening out the reason behind wrapping. Makes sense, but I didn’t want to assume anything.

I’ll try the 45 min sterilisation next time; thank you!

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