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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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Offlineshallow
member
Registered: 07/04/04
Posts: 229
Last seen: 8 years, 7 months
Day 4, no mycellium. Getting a little concerned
    #2886507 - 07/13/04 04:41 PM (12 years, 4 months ago)

Well, like I said its day 4 of incubation and not a sign of white is to be seen in any of my 6 jars. I used TC spores, jars were sterilized, neddle was sterilized, and the jars have been kept ion complete darkness at a temp ~76 degrees. No growth at all and no sign of contamination. What to do? Perhaps Im jumping the gun?


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OfflineVesicaP
Get ER Done

Registered: 07/06/04
Posts: 43
Loc: Here and Now
Last seen: 11 years, 5 days
Re: Day 4, no mycellium. Getting a little concerned [Re: shallow]
    #2886518 - 07/13/04 04:46 PM (12 years, 4 months ago)

You could be jumping the gun. I'm new too, but from what I've read it can take up to 10 days before you will see any myc. growth. you may want to bump your temp up to around 80 degrees. but that's the only thing i can tell ya.


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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: Day 4, no mycellium. Getting a little concerned [Re: shallow]
    #2886529 - 07/13/04 04:51 PM (12 years, 4 months ago)

do your jars look like they could be (too) dry?


--------------------
Man thinks. God laughs. - Jewish Proverb


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OfflineCyber
Ash
Male User Gallery Arcade Champion: Yeti Sports

Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
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Re: Day 4, no mycellium. Getting a little concerned [Re: shallow]
    #2886532 - 07/13/04 04:52 PM (12 years, 4 months ago)

Yep you are jumping the gun. Mine did not show for 7 days, and now (20+ days) I still have one that is only 5%.  My incubator is set at 82F.

For example, of 12 jars

4 are done and birthed

1 is done with the visible colonization and is on the 1st day of the 3day wait.

4 are 98% done and should be ready by the end of the week.

1 is 80% done

1 is 50% done

and 1 is 5% done and it just got a boost with a little myc injection :wink:


The one that is 80% did not start from the original spore injection but was injected at the 14 day mark with myc water. It took off and is growing FAST!


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Offlinecaimen
the unknown
Registered: 06/22/04
Posts: 184
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Re: Day 4, no mycellium. Getting a little concerned [Re: Cyber]
    #2887161 - 07/13/04 08:25 PM (12 years, 4 months ago)

Man make a culture It speeds up germation time  and safes on spores.
1 10cc  :syringe: will last for ever!


--------------------
MAN Made Speed
GOD Made Weed
<Who do YOU TRUST ? >

http://www.shroomery.org/index/par/25954


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Offlineshallow
member
Registered: 07/04/04
Posts: 229
Last seen: 8 years, 7 months
Re: Day 4, no mycellium. Getting a little concerned [Re: caimen]
    #2887175 - 07/13/04 08:29 PM (12 years, 4 months ago)

Care to point me toward a tut? Or...ummm...urr...A basic description. I just looked up culture in the tuts and dont quite understand.

Anyways yes, the mixture appears very moist. I dunno, gunna buils an incubator. Ive got more spores and rubbermaid so maybe a cultures the way to go.


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OfflinefIsh in my head
fadedstar

Registered: 01/25/03
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Re: Day 4, no mycellium. Getting a little concerned [Re: shallow]
    #2887240 - 07/13/04 08:47 PM (12 years, 4 months ago)



By the way 86F is the good incubator temp for fastest growth.

.fs.


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 3 years, 10 months
Trusted Cultivator
Re: Day 4, no mycellium. Getting a little concerned [Re: fIsh in my head]
    #2887570 - 07/13/04 10:52 PM (12 years, 4 months ago)

Its best to have it lower as myc generate their own heat 80-83 is generally considered optimal.


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OfflinefIsh in my head
fadedstar

Registered: 01/25/03
Posts: 1,050
Loc: 4500 ASL
Last seen: 10 days, 19 hours
Re: Day 4, no mycellium. Getting a little concerned [Re: scatmanrav]
    #2890510 - 07/14/04 05:59 PM (12 years, 4 months ago)


I'm afraid biological heating is not significant in a small quart jar. On the other hand, it becomes significant with spawn runs during large bulk projects.


But hey, anywhere between 80-86 will give similar results...

.fs.


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Offlinemr_X_
stranger danger

Registered: 07/11/04
Posts: 159
Last seen: 10 years, 9 months
Re: Day 4, no mycellium. Getting a little concerned [Re: shallow]
    #2890857 - 07/14/04 07:37 PM (12 years, 4 months ago)

relax it takes time..pluse like others have said pump up the heat.

good luck


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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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