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Offlinemycordia
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Registered: 12/19/23
Posts: 15
Last seen: 3 days, 6 hours
Bottle tek and one-in-all experiments
    #28785898 - 05/24/24 07:56 AM (1 month, 2 days ago)

I have had some disappointing experiences with contamination of my Mckennaii Cubensis bulk substrate recently. Initially I blamed the liquid culture but agar experiments showed no visible problems. Using the common workflow I really worked sterile. For now I suspect the bulk substrate (coco coir and vermiculite) and pasteurizing this is not sufficient. To proof this I started a bottle tek experiment inspired by https://www.shroomery.org/forums/showflat.php/Number/21168958
I used two different approaches based on jars prepared with an injection port and a PTFE membrane:
- vermiculite and a bran/wheat flour/gypsum mixture (PF tek like)
- coco coir, and bran/wheat flour/chicken pellets/catfood/gypsum
The jars have been sterilized for more than 2 hours on 15 psi.

I have 9 of these jars and after inoculating with the LC started colonizing well. Maybe I am impatient but I have the feeling the colonization is getting slower over time. I was thinking whether the anaerobic conditions in the jars may slow down the process. I made some markings in blue on the jars 4 days ago and it seems the colonization speed is only 1-2 mm per day.



When the colonization is finalized I intend to open the jars in a prepared monotub. What is your opinion on this experiment? Any advice?

Recently I prepared another experiment using selfmade one-in-all growbags. Maybe the colonization speed wil improve using rye and brake and shake the contents when this is colonized. I used LC and a syringe to inoculate the rye and covered this using micropore tape and a cover of multipurpose tape.


Edited by mycordia (05/24/24 07:59 AM)

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Onlinecybork
Stdt mycology
I'm a teapot Unread Journal User Gallery


Registered: 07/01/23
Posts: 366
Last seen: 4 minutes, 16 seconds
Re: Bottle tek and one-in-all experiments [Re: mycordia]
    #28785917 - 05/24/24 08:09 AM (1 month, 2 days ago)

Maybe I am impatient but I have the feeling the colonization is getting slower over time.
>>From the looks of you photo, giving them more easy gas exchange would help them. I mean: stuff the jar less full.

Or add another hole in the lid with such a breathable sticker.

Edited by cybork (05/24/24 08:23 AM)

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Offlinemycordia
Stranger
Registered: 12/19/23
Posts: 15
Last seen: 3 days, 6 hours
Re: Bottle tek and one-in-all experiments [Re: cybork]
    #28786030 - 05/24/24 09:55 AM (1 month, 2 days ago)

Yes, thats an easy Learning. Following the example I used quite a lot substrate.  Moreover I used wet vermiculite as a casing. It should be 4 or 5 cm less.

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