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PBJ710
Strangler


Registered: 07/05/19
Posts: 2,927
Last seen: 51 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: mikerec]
#28785270 - 05/23/24 05:01 PM (1 month, 3 days ago) |
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We both replied to Dkeppel - thats why it shows "Re: Dkeppel" at the end of the subject line.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,007
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Re: Ask Quick Questions, Get Quick Answers [Re: mikerec] 1
#28785273 - 05/23/24 05:05 PM (1 month, 3 days ago) |
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To Dkeppel and yes they do get confusing. This one moves pretty fast.
I looked at your earlier post and the grain did look bacterial. So top fruiting gives the best odds at success. They might have made it in coir, but breaking them up, forcing them to recover and providing that much moisture is a recipe for failure when the spawn is bacterial. Can it flush? Sure, but the odds are against you and it takes more more time, resources and space. So top fruiting is still the more likely chance for success with less wasted resources.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Dkeppel
Lurking Kiwi



Registered: 05/10/22
Posts: 118
Loc: New Zealand
Last seen: 14 minutes, 14 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28785276 - 05/23/24 05:09 PM (1 month, 3 days ago) |
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Quote:
PBJ710 said: They're toast...start a new project.
Quote:
ReverendMyc said: Yup. Sorry buddy, those are bacterial and possibly yeasty. They will likely be pretty smelly when you open them.
I just dumped 8 jars yesterday due to warped lids from overcooking. It happens.
As I suspected, thanks for your quick responses.
Project isn't a complete lost cause, it's just down sized to 4 shoe boxes instead of the planned 8
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Mr Piggy
bioilliminicent©️



Registered: 09/29/11
Posts: 9,676
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Re: Ask Quick Questions, Get Quick Answers [Re: Yuggoth] 3
#28785297 - 05/23/24 05:24 PM (1 month, 3 days ago) |
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Quote:
Yuggoth said: I never use mobile but the long signatures are a pain on desktop too. If you use the scroll wheel it "sticks" on scrolling signatures and interrupts your page scroll. I'm about to look for a setting to hide everyone's signatures.
I solved this by blocking all large sigs in the user's profile
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻 New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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mikerec
Stranger
Registered: 03/01/24
Posts: 30
Last seen: 11 days, 7 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28785322 - 05/23/24 05:38 PM (1 month, 3 days ago) |
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Quote:
ReverendMyc said: To Dkeppel and yes they do get confusing. This one moves pretty fast.
I looked at your earlier post and the grain did look bacterial. So top fruiting gives the best odds at success. They might have made it in coir, but breaking them up, forcing them to recover and providing that much moisture is a recipe for failure when the spawn is bacterial. Can it flush? Sure, but the odds are against you and it takes more more time, resources and space. So top fruiting is still the more likely chance for success with less wasted resources.
Ah, my bad. I see it now.
So, assuming the spawn is bacerial, what should I typically expect to happen? And at what stage?
I just assumed I'd get no growth at all and end up with a slimy, smelly mess with the way people replied.
But now I have 2 shoeboxes which seem to be colonizing fine. There's no color or odor. I thought it was a little wet initially, but it seems fine now. And now have pins in the jars and shoebox.
Should bacteria be causing issues yet?
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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
Posts: 12,907
Loc: PNW
Last seen: 1 hour, 4 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: mikerec]
#28785324 - 05/23/24 05:40 PM (1 month, 3 days ago) |
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You might get a couple fruits and then you will see a contamination eating away at your grow. Get what you can, but don’t expect a second flush.
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Dkeppel
Lurking Kiwi



Registered: 05/10/22
Posts: 118
Loc: New Zealand
Last seen: 14 minutes, 14 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
#28785331 - 05/23/24 05:48 PM (1 month, 3 days ago) |
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Smell wasn't too bad when dumping but I've had covid recently and my sense of smell is shit, definitely slimely inside those 2 jars.
One last dumb question.... what do you do about cleaning lids from bad grain jars?
They are a bit dirty, but I've read I shouldn't get the filters wet because it will ruin the filters integrity.
Do I rip off the filter and apply a new one or could I give the lid a wipe down and pressure cook the nastiness away i.e. wrap lids in foil and put in the instant pot for 3 hours?
Filters are a double layer of stiff felt, I have some 0.2µm borosilicate glass filters I could replace the felt with.
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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
Posts: 12,907
Loc: PNW
Last seen: 1 hour, 4 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
#28785332 - 05/23/24 05:50 PM (1 month, 3 days ago) |
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If you’re talking about jars that were contaminated. I replace those filters.
If the jar was able to produce good grain. I’ve heard people say they just directly put them back on to a jar and put them back in the pressure cooker.
But yeah, if you had contamination in the jar, I would not reuse that filter personally.
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StophJS
Padawan


Registered: 03/25/24
Posts: 108
Last seen: 43 minutes, 50 seconds
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We normally attribute contamination to originating from the spawn, but I have recently had what appears to be a small amount of trich on the very surface of my casing and seemingly nowhere else.
Thoughts?
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PBJ710
Strangler


Registered: 07/05/19
Posts: 2,927
Last seen: 51 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
#28785354 - 05/23/24 06:06 PM (1 month, 3 days ago) |
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Wash it with soap and water before re-using. There is absolutely no need to sterilize it again. If your filter can't handle getting wet, you should replace it or upgrade to an actual SFD that can be easily be cleaned.
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MrJong
Autismotronic 3000


Registered: 09/30/23
Posts: 771
Last seen: 23 hours, 54 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: StophJS]
#28785384 - 05/23/24 06:29 PM (1 month, 3 days ago) |
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Quote:
StophJS said: We normally attribute contamination to originating from the spawn, but I have recently had what appears to be a small amount of trich on the very surface of my casing and seemingly nowhere else.
Thoughts?
If you're 100% positive about this, your coir probably had some kind of nutritive stuff in that spot that the mold germinated on.
That's the best thing I can think of, especially if you heat treated your coir the tiniest bit yourself.
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alligator


Registered: 03/28/24
Posts: 199
Last seen: 3 days, 5 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: MrJong] 1
#28785414 - 05/23/24 06:56 PM (1 month, 3 days ago) |
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I have been enjoying PF Tek, so I'm going to stick with it for a while. I've already taken a couple spore prints and understand how to use them to clean up on agar and inject cakes with liquid or put the agar wedge directly in with no top layer of verm. Is there any reason to also get a clone of one of my better fruits too? Does it work the same as with spores as far as making transfers with the mycelium that grows and also inoculating the same as with spores? I just whipped up some new agar dishes last night and was reading some cloning teks and they don't really talk about what to do with the clones after the transfers and I wasn't sure if it was the same process, just with a different starting point.
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Phaethon
Stranger


Registered: 07/04/22
Posts: 222
Loc: Colorado
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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
#28785467 - 05/23/24 07:36 PM (1 month, 3 days ago) |
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Yep, you grow out clone cultures the same way you grow out multi-spore cultures. You can put an agar wedge of a clone culture to a BRF cake. You can put stem tissue directly to BRF cake to clone it, too (I've read about this but never done it personally).
Edit: to answer the question of whether there's a reason to clone, yes, if you want more consistency throughout a grow (similar phenotypes and maturation timelines). If you want a wide variety (e.g. hunting for interesting phenotypes or finding potent specimens - both of which you'd probably also want to clone), starting from spores gets you that.
Edited by Phaethon (05/23/24 07:41 PM)
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alligator


Registered: 03/28/24
Posts: 199
Last seen: 3 days, 5 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
#28785471 - 05/23/24 07:40 PM (1 month, 3 days ago) |
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Quote:
Phaethon said: Yep, you grow out clone cultures the same way you grow out multi-spore cultures. You can put an agar wedge of a clone culture to a BRF cake. You can put stem tissue directly to BRF cake to clone it, too (I've read about this but never done it personally).
Great, thanks! That's good to hear because I decided to just try it and dropped a couple on agar just now. I picked my biggest one I've had yet today so decided to give it a try.
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knupKlY
TamerOfPigeons


Registered: 09/05/22
Posts: 108
Last seen: 8 days, 49 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: alligator] 1
#28785622 - 05/23/24 10:20 PM (1 month, 2 days ago) |
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So I have already come to the conclusion that I need a SAB and actual PC to really boost my contam free results.
And honestly I’ve asked about coco so much but I want to ask on it a little more to understand and fully believe it ( I know you guys are far more experienced than I am and have more knowledge )
My first run since my new setup was two jars of rye grain which I spawned to a tub at the same time with brand new prepared coco coir ( boiling water into 5 gal bucket for 5 hrs ish )
The second run was another pair of rye grain jars which I spawned using the same coco coir which was still at field capacity and obv the lid has been opened since I used it to spawn the first run.
The First runs went perfect and got some flushes, the second run, well one contammed with trich and I just saw the smallest millimeter of trich on the side of the tub which I safely removed in hopes it’ll succeed.
So my question is could possibly opening that tub be the result of my problem aside from it being in the grain jar already? Or is the chance so minuscule that it shouldnt be a bother? Would I have to repasteurize the coco coir?
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 13,169
Loc: Hole
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Re: Ask Quick Questions, Get Quick Answers [Re: knupKlY] 6
#28785626 - 05/23/24 10:25 PM (1 month, 2 days ago) |
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It’s not the coir. It’s the spawn.
I keep my coir in buckets sometimes for a month+. There’s a post here detailing a guy keeping coir in an ice chest for over a year and it was fine.
From the moment you open your jar, spawn to bulk, or crack the lid on your coir bucket you are introducing spores and dust and bullshit from the outside world.
The only armor you have against that is clean, fully colonized grain spawn.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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knupKlY
TamerOfPigeons


Registered: 09/05/22
Posts: 108
Last seen: 8 days, 49 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 2
#28785630 - 05/23/24 10:33 PM (1 month, 2 days ago) |
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Just had to verify it was or wasn’t the Coco, thank you
Currently creating an SAB and ordering a PC to hope for better results, upset it wasn’t done sooner
Any links or direction towards someone who has a good PC guide with grain jars?
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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
Posts: 12,907
Loc: PNW
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Re: Ask Quick Questions, Get Quick Answers [Re: knupKlY]
#28785632 - 05/23/24 10:34 PM (1 month, 2 days ago) |
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Edited by GenesisCorrupted (05/24/24 12:04 AM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 13,169
Loc: Hole
Last seen: 38 seconds
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Are you PCing your grain for only 45 minutes?
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
Posts: 12,907
Loc: PNW
Last seen: 1 hour, 4 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28785643 - 05/23/24 10:46 PM (1 month, 2 days ago) |
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I usually do an hour. But that’s because I don’t trust my PC that much. I mean once you get to 15psi then 45 minutes.
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