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Offlinemush_monkey
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Can you tell if this is mold or tormentose mycelium?
    #28780822 - 05/20/24 02:50 AM (1 month, 6 days ago)

I think that if squint real hard I see that the cloudy part reaches the same cycle border as the healthy myc. Also it seems that it gets less cloudier and more filamentose (if that's even a word). Besides a healthy but seems to have grown out of the cloudy part



I have another plate that looks similar. Thanks in advance.


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P. Cubensis Odisha




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Offlinefiddle_head
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 2
    #28780862 - 05/20/24 03:57 AM (1 month, 6 days ago)

i would not send that. take a transfer from the most uniform leading edge, which is clear.

filamentous.


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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: fiddle_head]
    #28780942 - 05/20/24 06:41 AM (1 month, 6 days ago)

I'll make a second transfer (this was a transfer from a very clean clone). That will set me back a couple of weeks, so I think I'll try my luck with a little jar too. I mean, it's not 100% confirmed that it is mold, right?


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P. Cubensis Odisha




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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: fiddle_head]
    #28780983 - 05/20/24 07:34 AM (1 month, 6 days ago)

You wouldn't say this one looks any better, would you?



It's a transfer from the same clone


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P. Cubensis Odisha




Edited by mush_monkey (05/20/24 07:40 AM)

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OfflinePnin
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 2
    #28780999 - 05/20/24 07:52 AM (1 month, 6 days ago)

If you must make a jar, do it with a little wedge from six o clock on that plate would be my move.


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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: Pnin]
    #28781055 - 05/20/24 08:57 AM (1 month, 6 days ago)

:takingnotes:thank you!


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P. Cubensis Odisha




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Offlinevicepope
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey]
    #28781461 - 05/20/24 05:13 PM (1 month, 6 days ago)

Have you tried josex poke method, the agar color is messing with me. Makes it like bacteria there but can't tell.

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OfflineMacMerdin
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 2
    #28781609 - 05/20/24 06:58 PM (1 month, 6 days ago)

Quote:

mush_monkey said:
You wouldn't say this one looks any better, would you?



It's a transfer from the same clone





Looks like your clone wasn't as clean as you thought.  It appears to have cobweb mold to me.

Take a wedge from 6 o'clock like suggested


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Edited by MacMerdin (05/20/24 07:00 PM)

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InvisibleNichrome
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Re: Can you tell if this is mold or tormentose mycelium? [Re: MacMerdin] * 1
    #28781807 - 05/20/24 09:23 PM (1 month, 5 days ago)

It's just got a mono hitching along.

Transfer a piece of the fluff and see if it goes rhizo. Either a mono or it's a culture that is submissive to the other culture. The one thinks the other is more important so it is taking the back seat voluntarily. If it goes rhizo it'll fruit. If not it's probably a mono hitching.

Waiting in a fruit is exactly where I'd be if I was looking to score. Best chances of contact with new spores.


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question everything, it's very responsive



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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: vicepope]
    #28781925 - 05/21/24 02:10 AM (1 month, 5 days ago)

Tha josex LC Tek is a whole 'nother can of worms! So I guess I may just go ahead and try it:grin: And yeah my agar is embarrassing. Its BRF+honey, I finna make some LME plates next weekend (no pour this time)


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P. Cubensis Odisha




Edited by mush_monkey (05/21/24 02:27 AM)

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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: MacMerdin]
    #28781926 - 05/21/24 02:12 AM (1 month, 5 days ago)

You're most likely right, but why didn't I get the contamination in the clone? Healthy mycelium covered the whole plate


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P. Cubensis Odisha




Edited by mush_monkey (05/21/24 02:26 AM)

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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: Nichrome]
    #28781931 - 05/21/24 02:18 AM (1 month, 5 days ago)

That's a great idea, thank you. What is mono, btw? Search ain't helping, is it a mold too?


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P. Cubensis Odisha




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Offlinevicepope
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 2
    #28781961 - 05/21/24 03:36 AM (1 month, 5 days ago)

Mono as in monoculture. 1 culture genetically the same throughout. Most companies start with culture work for many transfers before getting it isolated to something they like, then transfer to deep slants, and culture tubes, and the rest to master lc, that them lead to secondary lc mass production, then selling the avg joe the secondly lc in syringes that cost them a few cents in material and 1 min of work making the syringe . Lots of money,  and I believe I remember you got your culture form a kit right?

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InvisibleNichrome
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Re: Can you tell if this is mold or tormentose mycelium? [Re: vicepope] * 4
    #28782440 - 05/21/24 12:17 PM (1 month, 5 days ago)

Quote:

vicepope said:
Mono as in monoculture. 1 culture genetically the same throughout. Most companies start with culture work for many transfers before getting it isolated to something they like, then transfer to deep slants, and culture tubes, and the rest to master lc, that them lead to secondary lc mass production, then selling the avg joe the secondly lc in syringes that cost them a few cents in material and 1 min of work making the syringe . Lots of money,  and I believe I remember you got your culture form a kit right?






Mono as in "monokaryon". Not monoculture whatever that is....

What are these "companies" you are referring to? Nobody was talking about companies or what they do and frankly companies have about the lowest standards in the myco world and whatever they are doing (which nobody outside the company really knows) should not be used as any standard. Very little of what you said here makes any real sense. WTF is "secondary LC"? Nobody does that...

OP your plate does not have mold or any other contamination. You have an axenic cubensis culture albeit genetically diverse still.


--------------------
question everything, it's very responsive



β€œDrink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.”
― Thich Nhat Hanh


Gypsum

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OfflineMrJong
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 1
    #28782479 - 05/21/24 12:47 PM (1 month, 5 days ago)

I second nichrome.

With mold you would've most likely already seen damage and those typical "separating" lines, I forgot how we call them, but basically when you have incompatible mycelium crossing together, they tend to make that defensive thick myc wall and it's usually very visual with mold
Besides, transfering a few times would've resulted in a plate covered in black spores or sum'in at that point

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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: vicepope]
    #28784073 - 05/22/24 03:25 PM (1 month, 4 days ago)

Yes, a colonized rye container cas d in verm.


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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: Nichrome]
    #28784540 - 05/23/24 02:19 AM (1 month, 3 days ago)

Done&done! Let's see what grows from that.

So, if I'm my reading comprehension hasn't failed me yet another time, a monokaryot mycelium is one germinated from a spore that hasn't mated and it's waiting for the opportunity to doing so (a mINCELium, get it?).

If that's the case, in the transfer I should expect the same type of nob filamentous growth, right?


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Offlinemush_monkey
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Re: Can you tell if this is mold or tormentose mycelium? [Re: MrJong] * 2
    #28793796 - 05/30/24 02:46 PM (27 days, 5 hours ago)

I transfered the mold looking part and got this:




Looks pretty healthy right? Or is the inner part weird?


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OfflineMacMerdin
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 1
    #28794784 - 05/31/24 08:05 AM (26 days, 12 hours ago)

Quote:

mush_monkey said:
I transfered the mold looking part and got this:




Looks pretty healthy right? Or is the inner part weird?





Looks good to me


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OfflineContactUFO
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Re: Can you tell if this is mold or tormentose mycelium? [Re: mush_monkey] * 1
    #28794860 - 05/31/24 08:58 AM (26 days, 11 hours ago)

Quote:

mush_monkey said:
I transfered the mold looking part and got this:




Looks pretty healthy right? Or is the inner part weird?




Looks good, i would do another transfer from the edge (8 o'clock)of the rhizo growth and you should be fine by then :smile:


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LAGM 2024


Edited by ContactUFO (05/31/24 09:04 AM)

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