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Offlinemush_monkey
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Rye is soaking for my first jar attempt.
    #28779415 - 05/19/24 01:32 AM (1 month, 8 days ago)

Plan was to inoculate them with agar. I took a two of biopsies a couple of weeks ago. One turned out great and made these transfers





I think there's mold in some areas. Can I use the healthy bitsany of them to inoculate the jar.

The other clone I made is out of the question, yes?



I guess I could use the first clone that looks great but I'm saving it in the fridge for a rainy day. Thanks in advance!


--------------------
P. Cubensis Odisha




Edited by mush_monkey (05/19/24 03:03 AM)

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Offlinecybork
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Re: Rye is soaking for my first jar attempt. [Re: mush_monkey] * 1
    #28779428 - 05/19/24 02:05 AM (1 month, 8 days ago)

If you suspect mold, better not use any of it to inoculate jar. Best is to use parts from completely clean agar dish to grain (jars). So, vector from your plate to another plate. And check how it goes there. Or take a chance and put already a piece to grain, if you can spend the effort.

I do think all plates look pretty nice. Not sure whether the fluffy is mold. But, I'm not a TC, so wait for some TC to comment on that.

:thumbup:

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OfflinePBJ710
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Re: Rye is soaking for my first jar attempt. [Re: cybork] * 1
    #28779446 - 05/19/24 02:49 AM (1 month, 8 days ago)

Transfer the culture UNTIL it has clean consistent growth.  The whole point of agar is to be able to get clean tissue away from any contaminant growth and isolate only that good growth.  Don't try to inoculate grains with them until you have dishes with only clean growth on them.

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Re: Rye is soaking for my first jar attempt. [Re: cybork]
    #28779453 - 05/19/24 03:06 AM (1 month, 8 days ago)

Thank you! I thought mycelium was not supposed to look fluffy, though .


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P. Cubensis Odisha




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Re: Rye is soaking for my first jar attempt. [Re: PBJ710]
    #28779454 - 05/19/24 03:15 AM (1 month, 8 days ago)

For how long can you keep sterilized grain jars before inoculation?

What I don't understand is that transfers from a pristine plate are moldy? Is that common or it's just that the universe hates me?


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OfflinePBJ710
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Re: Rye is soaking for my first jar attempt. [Re: mush_monkey]
    #28779468 - 05/19/24 04:06 AM (1 month, 8 days ago)

It can be fluffy and healthy - sometimes you may just have alot of cultures growing on top of each other, the nutritional content of the agar mix can do it or it's just the culture you got from the gentics lottery.  I rarey trust the first transfer and keep going until I get nice symmetrical growth before sending it to grain.

Ideally use grain asap, but it will last for months if it's not allowed to dry out.

How sure are you that the other plate was actually 'pristine' and not just white growth?  *IF* you are seeing mold (and not just tomatose mycelium), it came from somewhere...agar does a great job of allowing you to see where it originated at since growth is linear directly away from the source.

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Re: Rye is soaking for my first jar attempt. [Re: PBJ710]
    #28779480 - 05/19/24 04:39 AM (1 month, 8 days ago)

Well, it looked pristine to my weary noob eyes
( The one on the right)



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P. Cubensis Odisha




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Offlinemush_monkey
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50% of berries burst open [Re: mush_monkey]
    #28780759 - 05/20/24 12:07 AM (1 month, 7 days ago)

Can I still use it ?



It simmered for less than 10 minutes, I promise. Unless the difference between simmer and boil is like wet, moist an damp in them gardening YT videos in which it means something different every time.


--------------------
P. Cubensis Odisha




Edited by mush_monkey (05/20/24 01:31 AM)

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Offlinecybork
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Re: 50% of berries burst open [Re: mush_monkey] * 2
    #28780766 - 05/20/24 12:45 AM (1 month, 7 days ago)

I guess you mean whether it still can be used while it is overcooked?

If so: I guess you can use it (assuming you will sterilise too), but for my taste (pun intended) I would boil a new batch. The jelly structure of those broken berries behaves undesireable in a jar. It sticks to the glass. Also difficult to spot contamination. And I think mycelium find it easier to jump from fibre to fibre, instead of growing in mucus kind of substrate. A few mucus like ones I think is fine, but this batch seem to be too far.

So, my suggestion is you add some spice, veggies and some saus to the current batch and enjoy a healthy meal, and prepare a new batch for your jars.

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Re: 50% of berries burst open [Re: cybork]
    #28780805 - 05/20/24 02:15 AM (1 month, 7 days ago)

Thank you. I guess that's what I'll have to do. Tried it and doesn't taste that bad, I guess I can add it to a salad.


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P. Cubensis Odisha




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