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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,256
Loc: Zone 5
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chaos4142]
#28775263 - 05/15/24 02:15 PM (1 month, 11 days ago) |
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No they are not. I do not flaunt the term bacterial all willy nilly. If I thought it was such I would say so.
I'm not saying it is a good culture though. It is surely iffy in some ways but like I said that could be the media influencing growth as well as genetics. Only time will tell and I surely wouldn't send that yet. More transfers to good agar would be the course to take before betting the farm on it. I do see some conflict between emerging sectors there which in my opinion is harder to deal with than mold or bacteria. Internal non-cooperation is more of a psyhological occurrencer than cut and dry chemical reaction dictatorships.
-------------------- question everything, it's very responsive “Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh Gypsum
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Yahra
Meow


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] 1
#28775907 - 05/16/24 04:33 AM (1 month, 10 days ago) |
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How to clone this beauty? Just put the whole shroom on a new plate or tear it apart and use the inner tissue?
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fiddle_head
sporophyte



Registered: 08/05/08
Posts: 2,788
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] 1
#28775912 - 05/16/24 04:52 AM (1 month, 10 days ago) |
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Quote:
Yahra said: How to clone this beauty? Just put the whole shroom on a new plate or tear it apart and use the inner tissue?

I would just scrape a little off the outside with an inoculation loop. should be fine.
-------------------- lagm 2024 || lagm 2023 || 2023 || Things That Are Good ============= If you cannot afford petris you can still do this hobby. 🌿 Looking for spores? Look no further. 🐒
XI 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 XI
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Enjoil



Registered: 10/29/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head]
#28776463 - 05/16/24 01:58 PM (1 month, 10 days ago) |
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Hey guys have you ever had cultures that ate through the agar? I have a melmack culture that eats it like crazy and now I got a few pf jars about colonized. I plan on using one or two as master jars and knock up a few jars of rye to really get things jumping… I’m excited because my last grow was Costa Ricans and they dried up to almost nothing…
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AggroBot
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Enjoil] 1
#28777335 - 05/17/24 09:44 AM (1 month, 9 days ago) |
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Noob here… This agar started blue, all of them have turned this beige-ish color. I haven’t been able to find anything about that or whether I should be concerned.
Also is the slight darkness in the mycelium mold or contam of some sort?
Should I take a transfer off these, or send them, or trash ‘em?
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TPython
A Fraction of the Whole


Registered: 12/01/12
Posts: 801
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AggroBot]
#28777339 - 05/17/24 09:50 AM (1 month, 9 days ago) |
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Quote:
AggroBot said:


Noob here… This agar started blue, all of them have turned this beige-ish color. I haven’t been able to find anything about that or whether I should be concerned.
Also is the slight darkness in the mycelium mold or contam of some sort?
Should I take a transfer off these, or send them, or trash ‘em?
The changing color is from the mycelium eating the dye in the agar it’s all good.
What number transfer plates are those and are they cubes?
I’m not the best at identifying contams other than bacteria so I’ll leave that to someone with a more experienced eye. I’d take another transfer personally and see how they look
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Phaethon
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AggroBot]
#28777341 - 05/17/24 09:56 AM (1 month, 9 days ago) |
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Lots of mushroom species including cubensis will neutralize food coloring, nothing to worry about there.
The splotchiness of the mycelium worries me a little - you want nice uniform growth. Might be worth taking another transfer before sending. Or do both if you have extra grains and don't mind a potential failure.
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PBJ710
Strangler


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AggroBot] 1
#28777423 - 05/17/24 11:45 AM (1 month, 9 days ago) |
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Quote:
AggroBot said:
Noob here… This agar started blue, all of them have turned this beige-ish color. I haven’t been able to find anything about that or whether I should be concerned.
Also is the slight darkness in the mycelium mold or contam of some sort?
Should I take a transfer off these, or send them, or trash ‘em?
Plate 1 looks pretty sketchy and plate 2 looks like mold. If they are both cubensis, I would take another transfer from #1 before passing judgement and toss #2.
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Yuggoth
Mi-Go Cultivator


Registered: 03/04/23
Posts: 558
Loc: Lost Carcosa
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Re: AGAR ENVY! (Anything and All things agar!) [Re: PBJ710]
#28777452 - 05/17/24 12:13 PM (1 month, 9 days ago) |
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I would definitely transfer those. The growth is not awesome, but ... That's why we transfer.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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AggroBot
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yuggoth]
#28777461 - 05/17/24 12:21 PM (1 month, 9 days ago) |
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Awesome. Thanks everyone! I’ll try transferring them.
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Enjoil



Registered: 10/29/20
Posts: 2,616
Last seen: 5 hours, 40 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Enjoil]
#28777513 - 05/17/24 01:16 PM (1 month, 9 days ago) |
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Quote:
Enjoil said: Hey guys have you ever had cultures that ate through the agar? I have a melmack culture that eats it like crazy and now I got a few pf jars about colonized. I plan on using one or two as master jars and knock up a few jars of rye to really get things jumping… I’m excited because my last grow was Costa Ricans and they dried up to almost nothing…
Y’all have any cultures that ate to the bottom of the agar before it hits the side of the Petri dish? (I know I’m quoting myself because nobody responded….)thanks for any help guys! I love ya all!
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Yuggoth
Mi-Go Cultivator


Registered: 03/04/23
Posts: 558
Loc: Lost Carcosa
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AggroBot] 2
#28777549 - 05/17/24 01:50 PM (1 month, 9 days ago) |
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I have finally settled on a petri pouring method that I like. I'll share the highlights here. It's unconventional. It may be outright stupid. You may hate it. You may point and laugh. But it works for me!

Petri sterilization
I do dry oven sterilization of glass petris, because I hate condensation, and PC sterilization always results in some condensation on some plates. I store the foil-wrapped sterile stacks in a plastic tote until needed.
Agar prep
To make crystal clear agar, I actually do PC sterilization in two steps, each step more stupid than the last.
First, I process 1000 mL of nutrient solution in a large canning jar. This solution is just LME and whatever nutritional add-ons I intend to use. This jar of nutrient solution is shelf-stable because it is sterile. It will also have whatever kinds of crumbs and boogers the LME solution may produce when heated. (currently I am not using nutritional yeast or brewing yeast nutrient, but am experimenting with peptone as a supplement because it dissolves clear.)
When I want to actually make plates, I pour the sterile nutrient solution through a coffee filter and collect it in any convenient vessel. I try to leave the LME crud behind as much as possible, and I sacrifice some of the solution to do so. I aim for 750 mL of crystal clear nutrient solution in a 1000 mL media bottle.
I add a stir bar and add food coloring if I want. You can pour some solution out into a dish to check the color as you go, which is nice. Remember the LME solution is pre-darkened.
Then, the agar goes in the media bottle. I keep the stir bar on and let the agar bloom for 5-10 minutes. (I have seen this recommended in some documents, not sure it helps, but it doesn't hurt.)
Finally, I PC the agar as usual.
As soon as the agar bottle comes out of the PC I place it in a 60C water bath (sous vide gadget) and I just leave it alone until it hits that temperature, which is where I like to pour. You could cool the bottle faster, yes, but this is hands-off time so I just don't care if it takes a while.
You may think this is pretty stupid so far, but the extra stupid part is coming up.
Pouring
I unwrap a stack of plates in front of the FFU, and I put a few of them out horizontally. I uncover them one at a time, pour, and then leave them uncovered until they cool to under 70F as measured by an IR thermometer. That might take 5 minutes or more. Then I cover them and I have crystal clear and dry plates that are ready for storage OR immediate use. (If I store them, I do not wrap them, I just carefully transfer them to a plastic tote for storage in still air.)
I have mentioned my slow-pour method here before and it continues to work well for me.
Why am I so stupid?
- I really hate condensation and all the usual tricks like closing the dishes hot, stacking and waiting, reheating the tops, etc. don't seem to work for me. Look, I tried. Maybe I have weird petris or maybe I am too impatient. (I SAID I was stupid.)
- I really hate debris in the solution and all the usual tricks from pre-boiling with a stick blender to "LOL just PC it all at once and it comes out clear" were very inconsistent for me. (I SAID I was stupid.)
- I really hate to wait for plates to clear even if they cleared reliably because I am always in a hurry to transfer because I fucked up and waited too long with these other plates here, OK? (I SAID I was stupid.)
What I like about this method:
- Reliably crystal clear agar
- No condensation (I really hate condensation)
- Plates are immediately ready to use and pretty
- Easier to get the agar color you want, if you are into that
- You can make many agar bottles at once if you want, and you can re-melt it later ... And you can even stir it during melting without stirring up sediment, because there isn't any.
What I do not like about this method:
- Yes, agar prep is a little more work than "LOL just PC it all at once and it comes out clear" but if you make a bunch of shelf-stable nutrient solution jars at once, it's not much more work than a pre-boil/whisk method.
- It absolutely takes longer to pour the plates since you can only pour a few at a time and then you wait until they cool -- depends on your FFU workspace. Doing 100 plates this way would be whack. Doing 20 plates this way is fine. YMMV.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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mush_monkey
Stranger



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Can mycelium growth out of mold? [Re: Nichrome]
#28777556 - 05/17/24 01:56 PM (1 month, 9 days ago) |
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I have a plate with a clone that looks like first mold turning into mycelium

Also on the edge of the plate something fluffy appeared and mycelium is growing out of it.

Then I transfered a bit from a completely healthy mycelium and looks like this

One dude looks great while the other... Not so much. Is that normal?
-------------------- P. Cubensis Odisha
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,007
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yuggoth] 1
#28777587 - 05/17/24 02:31 PM (1 month, 9 days ago) |
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Quote:
Yuggoth said: I have finally settled on a petri pouring method that I like. ... Pouring
I unwrap a stack of plates in front of the FFU, and I put a few of them out horizontally. I uncover them one at a time, pour, and then leave them uncovered until they cool to under 70F as measured by an IR thermometer. That might take 5 minutes or more. Then I cover them and I have crystal clear and dry plates that are ready for storage OR immediate use. (If I store them, I do not wrap them, I just carefully transfer them to a plastic tote for storage in still air.)
I have mentioned my slow-pour method here before and it continues to work well for me. ...
Not to turn this into a confessional thread, but I hate condensation too. With prepours, there seems to be no avoiding it. I have been known to just open plates and set the lid at a tilt on the edge of the plate and let them sit in front of the ffu until dry.

An even worse crime is that I also have been having success with wiping the lids with paper towels sterilized in the jars with the no pours. Please don't take my shiny new badge away.
It works until it doesn't, so I am not endorsing it, just trying different things out.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Yuggoth
Mi-Go Cultivator


Registered: 03/04/23
Posts: 558
Loc: Lost Carcosa
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] 1
#28777592 - 05/17/24 02:35 PM (1 month, 9 days ago) |
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Thanks Rev, I love to see it!
> It works until it doesn't, so I am not endorsing it, just trying different things out.
You said it.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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Phaethon
Stranger


Registered: 07/04/22
Posts: 222
Loc: Colorado
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Enjoil] 1
#28778116 - 05/17/24 10:43 PM (1 month, 9 days ago) |
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Quote:
Enjoil said:
Quote:
Enjoil said: Hey guys have you ever had cultures that ate through the agar? I have a melmack culture that eats it like crazy and now I got a few pf jars about colonized. I plan on using one or two as master jars and knock up a few jars of rye to really get things jumping… I’m excited because my last grow was Costa Ricans and they dried up to almost nothing…
Y’all have any cultures that ate to the bottom of the agar before it hits the side of the Petri dish? (I know I’m quoting myself because nobody responded….)thanks for any help guys! I love ya all!
Mycelium runs through agar for sure. More noticeable with some cultures and species than others - some of my oyster cultures in particular make the agar kind of brittle (difficult to pick up large wedges without them breaking apart) once they've aged a bit.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Loc: USA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaethon]
#28779611 - 05/19/24 07:28 AM (1 month, 7 days ago) |
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,007
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper] 6
#28779774 - 05/19/24 09:28 AM (1 month, 7 days ago) |
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If you get bit by a dog you could rub that in the wound. It probably wouldn’t help, but you asked for thoughts, not good advice.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Last seen: 4 hours, 3 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
#28780574 - 05/19/24 07:52 PM (1 month, 7 days ago) |
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Hahahaha. Pretty bummed about this. Was stoked to get this culture so much. There’s more where it came from but the wait is 😞
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Mr Piggy
bioilliminicent©️



Registered: 09/29/11
Posts: 9,677
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] 3
#28780749 - 05/19/24 11:41 PM (1 month, 7 days ago) |
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Quote:
ReverendMyc said: It probably wouldn’t help, but you asked for thoughts, not good advice.

This has forever entered my lexicon, thank you.
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻 New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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