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Mc Mush
Stranger

Registered: 04/03/24
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Crystal clear LC turned yellow 2 months later
#28770204 - 05/11/24 06:47 AM (1 month, 15 days ago) |
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Good morning all,
I've got close to 16yrs of experience under my belt in this beloved hobby. In that time I've exclusively focused on active species, about 3 months ago I decided to play with medicinal and gourmet varieties. The reason for my post is to get an alternative opinion(s) on Liquid Cultures.
I have 1 Qt LC of Lions Mane that's in question. I made 6 Qt Liquid Cultures on 03/08/24. When they were made they were crystal clear and void of any sediment. Basically a perfectly made LC. Out of the 6 made that day I have successfully expanded all of them. Before I inoculated any grain, I first put the mycelium to agar to confirm that they're clean and void of contamination. All 6 were good to go. In the last couple months I've successfully colonized about 20lbs of rye and spawned to HWFP. Nothing contaminated and I've harvested my first flush already.
The concern I have is the Lions Mane LC went from clear (like water) to now clear yellow. It's not cloudy or hazy and doesn't have any indications of contamination, nor does the mycelium within. It just turned yellow, and all of my other LCs are crystal clear like water. I did a quality control the other day and again put them to agar. No signs of contamination yet and all have started to expand on the agar.
So I guess my question is.... has anyone ever seen anything like this before. This is a first for me.
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Smellyhobbit
Actual Retard


Registered: 04/01/22
Posts: 13,169
Loc: Hole
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Mc Mush]
#28770206 - 05/11/24 06:53 AM (1 month, 15 days ago) |
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This thread was moved from The Pub.
Reason: Just popping this over to mushcult
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,620
Loc: illinois
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Smellyhobbit]
#28770211 - 05/11/24 07:03 AM (1 month, 15 days ago) |
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prolly contamed but all u can do is test it now 16 years of growing
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Mc Mush
Stranger

Registered: 04/03/24
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Last seen: 1 month, 14 days
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: tripdawg420]
#28770232 - 05/11/24 07:25 AM (1 month, 15 days ago) |
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[Thanks for the reply. Yeah, I'm hoping it's not. The plates look fine so far. I even made up a small 150ml LC just to see if mabey the first 600ml LC ran out of nutrient, it was going good for like 30 days and then just stopped or stalled. The small 150ml is already expanding and is a different recipe with soy peptone, LME, yeast extract and Karo.
Is it possible that the Lions mane just didn't like the first LC and the yellow tint is metabolite?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,620
Loc: illinois
Last seen: 4 minutes, 29 seconds
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Mc Mush]
#28770234 - 05/11/24 07:27 AM (1 month, 15 days ago) |
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naw ur shits fucked
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Mc Mush
Stranger

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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: tripdawg420]
#28770265 - 05/11/24 07:43 AM (1 month, 15 days ago) |
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Thanks for the input. Shit is what it is, I guess.Its just weird tho, because the plates didnt show contamination of any kind. Good thing I've got master slants in the fridge then.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,620
Loc: illinois
Last seen: 4 minutes, 29 seconds
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Mc Mush]
#28770266 - 05/11/24 07:44 AM (1 month, 15 days ago) |
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,903
Loc: Canada
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Mc Mush]
#28770298 - 05/11/24 08:06 AM (1 month, 15 days ago) |
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This thread was moved from Mushroom Cultivation.
Reason: Gourmet.
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DcevLearn
Team Tomentose


Registered: 07/07/18
Posts: 363
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: tripdawg420] 1
#28770306 - 05/11/24 08:09 AM (1 month, 15 days ago) |
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Would help to know a breakdown of your process for making "perfectly made LC" and your process for retrieving the liquid once you're ready to use it. Maybe you introduced a contaminate when you took it to grain.
In the future you could keep an undisturbed amount as a baseline to see if it also goes yellow on its own.
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Mc Mush
Stranger

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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: DcevLearn]
#28770417 - 05/11/24 09:08 AM (1 month, 15 days ago) |
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There's several recipes for an LC, but a perfect LC... 2-4% nutrient by volume pH adjusted if need be 0ppm H20 Clear Transparent without sediment
Procedurally when doing any work I use a small laminar, iso and an induction sterilizer. LC is sprayed with iso,stirring on a plate. I'll wipe off the injection port and 45 the jar when filling the syringe.
If it is contaminated, then only thing that could have been the cause is the syringe itself. I PC my needles and syringes a couple times before I toss them. My Port's are a bit used too...
I have 2 tests and 1 control currently. But ultimately I have a clean pure isolate of every species I currently have in storage.
It's just odd, because a bacterial contaminated LC go cloudy. This one is completely transparent and looks healthy. It also doesn't have any off smells either.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,903
Loc: Canada
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Mc Mush] 2
#28770572 - 05/11/24 11:43 AM (1 month, 15 days ago) |
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2-4% is far from perfect, just saying.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 13,169
Loc: Hole
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Stipe-n Cap] 2
#28770575 - 05/11/24 11:47 AM (1 month, 15 days ago) |
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.2% nutrient, .2% agar Distilled water Sneeze into it while carrying it to the table accidentally Think about cleaning the dried mycelium from before out of the bottle Dont Microwave it until dissolved even though you know you literally don’t have to PC at 15-17 psi for 20 minutes but take a shit at the 19 minute mark so it’s in there for an extra 5-10 minutes depending on how much coffee you had Take PC off heat Forget about it for a day Remove media bottle and store it for 4 weeks until you remember you were trying to make LC See sediment Wonder where it came from Use anyway
That’s the perfect LC workflow
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,620
Loc: illinois
Last seen: 4 minutes, 29 seconds
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Re: Crystal clear LC turned yellow 2 months later (moved) [Re: Smellyhobbit]
#28770577 - 05/11/24 11:48 AM (1 month, 15 days ago) |
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i just use premix idk
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