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ansr
Strangers

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Re: Ask Quick Questions, Get Quick Answers [Re: Fishy247]
#28765215 - 05/07/24 12:11 PM (1 month, 19 days ago) |
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Have anyone tried washing petri dishes and wipe them with 70% isopropyl and use them again, just to see if it works? Are they really unusable?
I'm brand new to this so I don't know how stupid this question is.
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alaskappalachian
Entitiologist

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Loc: The 49th Dimension
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] 2
#28765219 - 05/07/24 12:18 PM (1 month, 19 days ago) |
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There are no stupid questions in this thread . Wiping down anything with ISO is sanitization, which is not the same as sterilization. It won't work, although you may get by with 1/100 plates growing usable myc (a fluke rate, not a success...). I use reusable PP5 containers which have saved me thousands in petri costs. I use these: https://www.amazon.com/dp/B0795CHXPR?ref=ppx_pop_mob_ap_share&tag=theshro-20 Burn a little hole and cover with two little pieces of micropore tape to prevent implosion during sterilization, and Bob's-your-uncle. They stack well in a PC and offer perfectly adequate viewing. (No-pour method)
    Just a suggestion though. Plenty of options available including glass petris if you want to be a Rockefeller.
-------------------- THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES "It is all one vast awakened thing. I call it the golden eternity. It is perfect." -Kerouac
Edited by alaskappalachian (05/07/24 12:28 PM)
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TPython
A Fraction of the Whole


Registered: 12/01/12
Posts: 801
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
#28765221 - 05/07/24 12:22 PM (1 month, 19 days ago) |
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Quote:
ansr said: Have anyone tried washing petri dishes and wipe them with 70% isopropyl and use them again, just to see if it works? Are they really unusable?
I'm brand new to this so I don't know how stupid this question is. 
You would need to pressure cook them for them to be sterile again.
But yes they are reusable as long as they’re glass or PP5 plastic
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Psilosion


Registered: 03/23/17
Posts: 579
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Quote:
AspectOfTheCreator said: Rate my grain.
Ill point out ahead of time that many are burst and it looks a bit wet... but the myc seems to be moving fast and loving it. It shakes well too.

Looks good, it's always difficult to get perfectly hydrated grain when it's all difference sizes. If it shakes well and you got clean growth, sounds great.
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AspectOfTheCreator
Mastering the Art of Success



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Re: Ask Quick Questions, Get Quick Answers [Re: Psilosion]
#28765236 - 05/07/24 12:38 PM (1 month, 19 days ago) |
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Quote:
Psilosion said:
Quote:
AspectOfTheCreator said: Rate my grain.
Ill point out ahead of time that many are burst and it looks a bit wet... but the myc seems to be moving fast and loving it. It shakes well too.

Looks good, it's always difficult to get perfectly hydrated grain when it's all difference sizes. If it shakes well and you got clean growth, sounds great.
Problem is I grow almost exclusively PE types, which means a long wait time before I get fruit. Sometimes things will go smoothly then just contam right when I expect fruit, or after the first flush. It doesn't happen TOO often but I certainly feel like I should be able to increase my success rate. Grain prep is the only thing I can trace my issues back to.
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ansr
Strangers

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Quote:
alaskappalachian said: There are no stupid questions in this thread . Wiping down anything with ISO is sanitization, which is not the same as sterilization. It won't work, although you may get by with 1/100 plates growing usable myc (a fluke rate, not a success...). I use reusable PP5 containers which have saved me thousands in petri costs. I use these: https://www.amazon.com/dp/B0795CHXPR?ref=ppx_pop_mob_ap_share&tag=theshro-20 Burn a little hole and cover with two little pieces of micropore tape to prevent implosion during sterilization, and Bob's-your-uncle. They stack well in a PC and offer perfectly adequate viewing. (No-pour method)
    Just a suggestion though. Plenty of options available including glass petris if you want to be a Rockefeller.
I was thinking since our gloves aren't PC'ed and the surfaces aren't PC'ed but only cleaned with isopropyl. But really only one out of 100, if you get lucky? So if we swab the surfaces we work on that have been wiped with isopropyl and test it on agar, it would be fully contaminated?
I got petri dishes when I started a month ago but I f'ed up everything since it was my first time and then got similar cups to those but they are so frosty it's hard to see through them. I can't get those specific ones you linked to because in Europe.
I struggled for a month and just got my first mycelium in PF tek jars the other day. It was really exciting to see it.
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Scemilope
Mycoheretic



Registered: 11/16/22
Posts: 800
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] 1
#28765244 - 05/07/24 12:48 PM (1 month, 19 days ago) |
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Quote:
ansr said: Have anyone tried washing petri dishes and wipe them with 70% isopropyl and use them again, just to see if it works? Are they really unusable?
I'm brand new to this so I don't know how stupid this question is. 
Welcome to the party! 🎉 I have reused plastic petri dishes. I washed them and sanitized them with peroxigard/REScue wipes inside my SAB. It was mostly successful, but they won't be sterile just well sanitized. I have also used 4oz jars for pre/no pour agar, they do fine but are a little awkward to work in. Ultimately I invested in some 75mm glass dishes and those have been great to work with.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,007
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] 2
#28765250 - 05/07/24 12:59 PM (1 month, 19 days ago) |
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There are also autoclavable plastic petris. The advantages of glass and plastic combined.
Also, it is easy to do a quick test. Swab some iso wiped items on a petri plate and wait. I have accidentally touched my iso soaked nitrile glove to the surface of plates and get a contam finger print every time. I mark the lid where I touch them to remember what happened. 100% contam in a couple of tests.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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alaskappalachian
Entitiologist

Registered: 10/22/19
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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] 1
#28765254 - 05/07/24 01:03 PM (1 month, 19 days ago) |
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@ansr So when we work in a SAB for instance, we are using mechanical principles to handle sterile agar plates (what's inside is sterile if done properly) in a way which doesn't introduce contaminants. Nothing else needs to be sterile, only sanitary. Work done in open air will have a ridiculous contamination rate, although people starting out often will do what they do and flame a spore syringe needle in their kitchen and inject a sterilized pf jar and get some wins; but that is due to luck and the fact that the vector is insanely small. Yes - if you swab anything at all that isn't sterile and introduce it to agar you will get a contaminant expressing in short order. Rather it's a bacterial bloom or a mold, it'll be there. Microbes are ubiquitous. In Europe I know it's often easier to get little jam jars and condiment jars. They make beautiful little dishes. With the European style lids that have like one tiny thread, they can be modified to not seal shut when PCing by modifying the lids like the sides of the PP5 dishes I pictured.
-------------------- THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES "It is all one vast awakened thing. I call it the golden eternity. It is perfect." -Kerouac
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ansr
Strangers

Registered: 04/25/24
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Interesting. I just remembered this video: He uses isopropyl to clean the glass and they turned out fine? Is that not a big risk? Well, one got contaminated but I thought that's normal no matter how clean you are?
I got some plastic cups but the lids aren't PP5 so the lids got deformed the first time I tried because I didn't make a hole in them, the second time I did make a hole in the lids but after PC'ing them the lids got hard and they crack a lot when trying to open them.
Do you mean glass jars is easier to get in Europe?
Edited by ansr (05/07/24 01:33 PM)
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ansr
Strangers

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28765279 - 05/07/24 01:28 PM (1 month, 19 days ago) |
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Quote:
ReverendMyc said: There are also autoclavable plastic petris. The advantages of glass and plastic combined.
Also, it is easy to do a quick test. Swab some iso wiped items on a petri plate and wait. I have accidentally touched my iso soaked nitrile glove to the surface of plates and get a contam finger print every time. I mark the lid where I touch them to remember what happened. 100% contam in a couple of tests.
I will do that just because it's fun to see what happens.
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tripdawg420
low life with no life



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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] 1
#28765283 - 05/07/24 01:31 PM (1 month, 19 days ago) |
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Quote:
Mr Piggy said: Why are you opening the tubs when you already have air exchange holes drilled in it?
exchanging the enviorment a few times aday resets the tub where evaporation will increase that being a big pinning trigger mushrooms love that fresh reset
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Mr Piggy
bioilliminicent©️



Registered: 09/29/11
Posts: 9,678
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Re: Ask Quick Questions, Get Quick Answers [Re: tripdawg420] 1
#28765297 - 05/07/24 02:00 PM (1 month, 19 days ago) |
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Absolutely not what's going on though.
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻 New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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tripdawg420
low life with no life



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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] 1
#28765300 - 05/07/24 02:03 PM (1 month, 19 days ago) |
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mushrooms dont grow in caves they grow in feilds with the evoirment changing constantly fat healthy fruits
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GenesisCorrupted
Taoist, Writer, Student, Artist




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Re: Ask Quick Questions, Get Quick Answers [Re: tripdawg420]
#28765302 - 05/07/24 02:05 PM (1 month, 19 days ago) |
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If they’re in the perfect temperature and humidity with enough air exchanged. They have any source of light. You don’t really need to do anything. I’ve seen mushrooms grow in places that don’t get any sunlight at all.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,034
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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] 3
#28765304 - 05/07/24 02:06 PM (1 month, 19 days ago) |
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Quote:
ansr said: Have anyone tried washing petri dishes and wipe them with 70% isopropyl and use them again, just to see if it works? Are they really unusable?
I'm brand new to this so I don't know how stupid this question is. 
This is a pic (from 2lazy2bee) of a thumbprint on agar, wearing gloves sprayed with 70% iso.
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Mr Piggy
bioilliminicent©️



Registered: 09/29/11
Posts: 9,678
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Re: Ask Quick Questions, Get Quick Answers [Re: tripdawg420] 3
#28765307 - 05/07/24 02:07 PM (1 month, 19 days ago) |
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We are not mimicking natural environments, we are creating optimal artificial environments. We are growing domesticated varieties indoors in plastic tubs on grain that is suspended in coconut husks. Natural is a fallacy.
He is here because opening his tub and airing it out like he has been doing is hurting his yields. The best growers here set their tubs and leave them, and that's that.
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻 New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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tripdawg420
low life with no life



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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
#28765309 - 05/07/24 02:08 PM (1 month, 19 days ago) |
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ur trying to give then a eviorment close as u can to out door think about it
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rumfor69
Bodhicitta Cultivator



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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] 1
#28765313 - 05/07/24 02:09 PM (1 month, 19 days ago) |
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If I couldn't just set it and forget it I dunno if I'd do this stuff much lol
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tripdawg420
low life with no life



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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#28765315 - 05/07/24 02:10 PM (1 month, 19 days ago) |
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nothing in this world is set and forget
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