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Bunnykinz
shroomer


Registered: 06/09/23
Posts: 54
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Re: Ask Quick Questions, Get Quick Answers [Re: MrJong]
#28751212 - 04/26/24 10:04 PM (1 month, 30 days ago) |
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This tubs has been like this for a few days, some of the first pins maybe a week. Seems extremely slow growth right? It's a lucid gates culture. Almost seems like it's a mutant grow, but hoping for normal fruit. So conditions seem right? It's a show box that I've kept closed and was opening once a day for a quick fan, but have left it closed lately as I think it gets sufficient FAE for the moment.

-------------------- “Nature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up. This is the trick. This is what all these teachers and philosophers who really counted, who really touched the alchemical gold, this is what they understood. This is the shamanic dance in the waterfall. This is how magic is done. By hurling yourself into the abyss and discovering it's a feather bed.” ― Terence McKenna
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MrJong
Autismotronic 3000


Registered: 09/30/23
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz]
#28751220 - 04/26/24 10:07 PM (1 month, 30 days ago) |
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Just looks a bit dry to me (clear colored, dry caps). Have you misted any of those times you've fanned?
Don't fan by the way. You want passive FAE
I'd try giving them a pretty strong mist, putting the lid back on and leaving them alone, just to see if they grow out more then. If they do, you'll likely have to rehydrate the substrate
You could just put your finger in there and try to see if the coir is moist too.
Edited by MrJong (04/26/24 10:13 PM)
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Bunnykinz
shroomer


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Re: Ask Quick Questions, Get Quick Answers [Re: MrJong]
#28751234 - 04/26/24 10:24 PM (1 month, 30 days ago) |
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Ok, I haven't been misting at all. When fanning I really just mean 5-10 seconds/day of lid off with a little blast from a computer fan. The substrate is moist still on tap, the little caps no so much. Isn't there a risk of causing aborts when misting pins? Or maybe I could most the lif just to increase humidity in there?
-------------------- “Nature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up. This is the trick. This is what all these teachers and philosophers who really counted, who really touched the alchemical gold, this is what they understood. This is the shamanic dance in the waterfall. This is how magic is done. By hurling yourself into the abyss and discovering it's a feather bed.” ― Terence McKenna
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AspenExtreme
Stranger

Registered: 02/18/24
Posts: 7
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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: DERRAYLD]
#28751330 - 04/26/24 11:53 PM (1 month, 30 days ago) |
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Post deleted by AspenExtreme
Reason for deletion: Why not?
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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
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Re: Ask Quick Questions, Get Quick Answers [Re: AspenExtreme]
#28751332 - 04/26/24 11:54 PM (1 month, 30 days ago) |
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But what strains was the gypsum being used for?
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vicepope
The devils best grower



Registered: 04/15/24
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Loc: The fear that burns in the hea...
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Quote:
GenesisCorrupted said: But what strains was the gypsum being used for?
In the grand scheme of things I only tested it with an isolated monoculture of phenotype south America. Back in 2015. Wish I had read aspen extreme post more. Question. Why is my inside grown Gymnopilus junonius lower in bis-noryangonin then its parent clone in the wild.
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AspenExtreme
Stranger

Registered: 02/18/24
Posts: 7
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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: vicepope]
#28751349 - 04/27/24 12:15 AM (1 month, 30 days ago) |
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Post deleted by AspenExtreme
Reason for deletion: Wrong
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vicepope
The devils best grower



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Re: Ask Quick Questions, Get Quick Answers [Re: AspenExtreme]
#28751357 - 04/27/24 12:25 AM (1 month, 30 days ago) |
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Just that the fruits where darker then there non gypsum counter parts. Not a big deal. I tested using ground poppy seeds as substance that yielded better then straw with same iso . Genetics are the key more then anything else. If you have clean culture and clean spawn you can do almost anything. It wasn't 10% gypsum it was only 3% by dry weight. If you want to expand on future grows,I recommend a counter caking agent like composted chicken manure pellet about 5% by weight and actually pasteurization method used. Otherwise yes commonly straight coir is the way to go when testing other variables of unknown.
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MrJong
Autismotronic 3000


Registered: 09/30/23
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz]
#28751377 - 04/27/24 01:03 AM (1 month, 30 days ago) |
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Quote:
Bunnykinz said: Ok, I haven't been misting at all. When fanning I really just mean 5-10 seconds/day of lid off with a little blast from a computer fan. The substrate is moist still on tap, the little caps no so much. Isn't there a risk of causing aborts when misting pins? Or maybe I could most the lif just to increase humidity in there?
Unless you literally drown them, no, the first thing you'll see is mycelium popping out the cap or a wet leathery aspect/darkening if you make them overly wet I regularly dunked my cakes with pins on them for near 24 hours, if anything they seemed to like it I like to think they're a bit smarter on resource management than we think, if they pin heavily but run out of sufficient moisture they just stop spending energy instead of wasting it and mass aborting or sending out a single fruit
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alligator

Registered: 03/28/24
Posts: 199
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Re: Ask Quick Questions, Get Quick Answers [Re: MrJong]
#28751491 - 04/27/24 06:04 AM (1 month, 29 days ago) |
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Hello all. Today is the day I plan start with agar. When you all PC your agar, do you try to throw as much other things in there as possible to sterilize or do you just wipe with ISO and flame sterilize anything that comes in direct contact with the agar?
I have sterile, individually wrapped blades, but my scalpel handle isn't sterile. Neither are my loop or syringe needles (I got all metal, blunt tipped so they could be reusable). Should I just wrap all those things up in aluminum foil and PC along with the agar?
And in your experience, is there anything I'm forgetting that you would also sterilize? I would like to only run the PC once if possible and it's going to be mostly empty.
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alligator

Registered: 03/28/24
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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
#28751492 - 04/27/24 06:06 AM (1 month, 29 days ago) |
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I should have mentioned, I am doing pour agar, so I got pyrex media bottles and sterile petri dishes.
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3.A.M
Boop!



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Re: Ask Quick Questions, Get Quick Answers [Re: alligator] 1
#28751495 - 04/27/24 06:12 AM (1 month, 29 days ago) |
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You don’t need to pc your tools, just wipe them down with iso and flame sterilise anything making direct contact with myc and agar, cool it in the agar itself before using.
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ContactUFO
MenMadeMushroom



Registered: 02/26/24
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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
#28751496 - 04/27/24 06:13 AM (1 month, 29 days ago) |
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I only sterilize the agar. My scalpel handle i just wipe down with iso when using it.
I always use new scalpel blades and flame sterilize them with a burner. No need to put them in the PC.
Also grains i wouldnt sterilize in the same run, as they need much longer PCing. Agar only needs 20 min at 15psi.
What i can give you from my learnings: fill the bottles only 50% to prevent spillover. Also, before pcing, really dissolve the agar in boiling water for 2-3 mins (attention, agar has strange behaviour when boiling)
After boiling, strain it through a towel to get rid of any leftover specks. I always have some brown specks (caramelized sugar i guess) in some of my plates, but they're not a problem at all..just no so appealing to look at
-------------------- LAGM 2024
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alligator

Registered: 03/28/24
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Re: Ask Quick Questions, Get Quick Answers [Re: ContactUFO]
#28751502 - 04/27/24 06:19 AM (1 month, 29 days ago) |
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Great, thank you for the advice! I will be working inside a SAB, so I wanted to be as clean and sterile as possible, but sounds like I was going overboard. I actually got a 2-pack of the media bottles, so filling them both halfway will not be a problem.
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tree frog
eats bugs



Registered: 09/14/23
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Re: Ask Quick Questions, Get Quick Answers [Re: vicepope]
#28751536 - 04/27/24 06:50 AM (1 month, 29 days ago) |
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Quote:
vicepope said: It wasn't 10% gypsum it was only 3% by dry weight.
I use gypsum primarily to prevent caking. Pretty minimal percentage. I can't afford 10% by dry weight.
It helps keep my casings fluffy, which I'm sure improves pinsets and fruit body formation. I also use it in compost spawn jars, as they're prone to caking.
Anyway for me using it is personal preference. I find it helpful in getting the substrate texture that I want. And it apparently adds calcium to the fruits (though edible growers probably care more about that).
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alligator

Registered: 03/28/24
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
#28751557 - 04/27/24 07:17 AM (1 month, 29 days ago) |
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I just went down a SHIP rabbit hole. Is there any reason I couldn't just ditch the SHIP and pop open a lid to inject using PF Tek too? The dry verm layer is supposed to act as a filter anyway, right?
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tree frog
eats bugs



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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
#28751561 - 04/27/24 07:22 AM (1 month, 29 days ago) |
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Are you inoculating an open air or in a still air box?
If the later then yeah definitely pop open the lid and squirt it in. If it's the former then you are going to be better off with the self healing injection port.
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Smellyhobbit
Actual Retard



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Re: Ask Quick Questions, Get Quick Answers [Re: alligator]
#28751563 - 04/27/24 07:26 AM (1 month, 29 days ago) |
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Quote:
alligator said: I just went down a SHIP rabbit hole. Is there any reason I couldn't just ditch the SHIP and pop open a lid to inject using PF Tek too? The dry verm layer is supposed to act as a filter anyway, right?
You shouldn’t need a SHIP or to open a lid for PF tek. There should be at least 1 hole in your lid for gas exchange that stays open.
You heat your needle and push it past the dry verm barrier into your cake.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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alligator

Registered: 03/28/24
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
#28751564 - 04/27/24 07:28 AM (1 month, 29 days ago) |
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I built a SAB and have done everything in that so far. I used the SHIPs in the SAB last time, but feel like with my wonky drill job for the holes, I'd be better off just using the lids and not having a possible contamination point (I used the black plugs for SHIPs).
This is great! I love when conventional wisdom and less work collide.
Edited by alligator (04/27/24 07:29 AM)
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Mackload
Big Mack


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Re: Ask Quick Questions, Get Quick Answers [Re: MrJong]
#28751588 - 04/27/24 07:55 AM (1 month, 29 days ago) |
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Quote:
MrJong said:
Quote:
Phaethon said: I've had plates sit wrapped for months and stay sterile and usable. 
Was it mold or bacteria you found?
I do think the common knowledge around sterilization time for agar media tends to be a bit optimistic. I go for 45 minutes with media bottles filled with 400 mL.
Depends on the bacteria you have in there too I guess. Initially I had no problem but then ran into some heat resistant bacteria that'd survive steaming agar for over an hour. Went away with the PC. The ones that survive the higher end of the temp spectrum are much fewer and far between normally.
That shit drove me insane, I became paranoid as fuck with my technique which turned out to be just fine (I've been much more sloppy since with no issues). I was wondering why the fuck I'd keep having them all over under the agar. Not mold a single time. Questioned petri vendors and changed provider to check. Was the sterilization.
Both, a few mold, a few bacterial. I'm using *cough* an instant pot to pressure cook, so perhaps I'll up the time there.
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