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Invisiblecheesenoonions
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Registered: 04/02/01
Posts: 584
Any thoughts on 5HT as additive
    #2875004 - 07/10/04 02:37 AM (12 years, 5 months ago)

A friend has access to 10 mM serotonin. Possible problems...
1. Not sterile and i don't think it can be autoclaved
Maybe filter sterilize and add antibiotics
or add the serotonin after the cake is colonized
2. .Experiment aould be difficult to set up.

So I'm thinking that casings would be too large. One 1/2 pint cake with 1mL should be enough. Any suggestions for setting up an experiment. I can't eat the mushrooms since that would be subjective and I can't assay the actual contents.


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OfflineBlue_Apocalypse
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Re: Any thoughts on 5HT as additive [Re: cheesenoonions]
    #2875042 - 07/10/04 02:54 AM (12 years, 5 months ago)

I'd say add it using aseptic technique to the jar just after the mycelium starts to germinate. If you incubate, you should be able to colonize at least 6-8 out of 10 jars. Maybe dilute it in a solvent first. I dont know much about the psilocybin pathway so im not sure what effect it would have, if any.

Really, the only experimentation you can do is subjective unless you have an HPLC at your disposal. Your best bet would be to simply grow out an experimental and control group and then test the qualitative properties yourself.


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Invisiblecheesenoonions
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Re: Any thoughts on 5HT as additive [Re: Blue_Apocalypse]
    #2875051 - 07/10/04 02:59 AM (12 years, 5 months ago)

I once read something in a book called "psilocybin production", or something similar thta during extraction you can check for the presence of actives with reagents. there's got to be a way, otherwise it'll just be me saying "dude, trust me, they were the bomb!" how many times have we heard that regarding potency.


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OfflineBlue_Apocalypse
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Re: Any thoughts on 5HT as additive [Re: cheesenoonions]
    #2875114 - 07/10/04 03:27 AM (12 years, 5 months ago)

Yes you can check for active chemicals but you cannot measure how much is there unless you have at least a UV detector and probably some other stuff. I suppose you could try extracting psilocybin from both samples using the same technique and weighing it, but the margin of error will probably be larger than the difference that the serotonin would make. Actually i dont think serotonin is metabolized into psilocybin but i may be wrong.


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Invisiblejonneill
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Re: Any thoughts on 5HT as additive [Re: Blue_Apocalypse]
    #2875123 - 07/10/04 03:33 AM (12 years, 5 months ago)

Psilocybin is made from DMT through 4-hydroxylation. I don't think serotonin would be converted into any actives by the fungus.


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Invisiblecheesenoonions
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Re: Any thoughts on 5HT as additive [Re: jonneill]
    #2875165 - 07/10/04 03:56 AM (12 years, 5 months ago)

I just figured since 5HT is smilar to DMT and given that the enzymes involved in the latter stages of the process are unkown, it was worth a shot. DMT has a high affinity for serotonin receptors. If these receptors have difficulty discerning b/w 5HT and DMT it's not too much of a stretch to think the fungus will do the same. no?


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OfflineBlue_Apocalypse
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Re: Any thoughts on 5HT as additive [Re: cheesenoonions]
    #2875800 - 07/10/04 01:42 PM (12 years, 5 months ago)

Well The mushroom would have to have an enzyme or chain of enzymes to convert the 5HT to DMT first because the hydroxylase enzyme is probably only capable of that one chemical conversion.


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Invisiblecheesenoonions
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Re: Any thoughts on 5HT as additive [Re: Blue_Apocalypse]
    #2876499 - 07/10/04 08:05 PM (12 years, 5 months ago)

good point


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