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OfflineDj_karma_magnet
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Re: Cultivation General Discussion [Re: Mr Piggy]
    #28734022 - 04/11/24 04:44 PM (2 months, 13 days ago)

Is it okay if I put bags of colonized grains in the freezer and save them for a few weeks? I’ve heard this is possible and won’t affect quality but have my doubts. The bags do have micron patches.

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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Dj_karma_magnet] * 3
    #28734033 - 04/11/24 04:49 PM (2 months, 13 days ago)

Fridge not freezer


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InvisibleScrimshaw
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Re: Cultivation General Discussion [Re: A.k.a]
    #28734762 - 04/12/24 05:54 AM (2 months, 12 days ago)

When you guys inoculate bags with LC, do you bother trying to mix the grain before, after, or not at all after pouring?

Since I don’t preseal bags before PC I find it difficult to mix up the grain before inoculating. This often leaves some spots of wetter vs dryer grains distributed through the bag. Add in LC and there’s even more inconsistency with moisture. I’ve read posts saying don’t mix after adding LC, but I don’t really want those pockets of moisture inherent in the bag at that point.

If I shake after inoculating the bags look great and consistent, but I don’t want to be slowing or weakening growth if I don’t have to be.

I’ve shaken, not shaken, and everything in between and I still get growth. I’m just curious on what the current prevailing thoughts are on the topic.

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Scrimshaw] * 1
    #28734783 - 04/12/24 06:48 AM (2 months, 12 days ago)

with lc i never shake after inoculation. i want to see the LC colonize the grains and if i see mold its easier to point point where it came from. once the lc has recovered and i can see it cleanly colonize the grains? thats when i shake. jars or bags.

also if using a good amount of liquid? i like to prep the grains a bit drier for similar reasons you pointed out.

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Onlinehamloaf
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #28734788 - 04/12/24 07:04 AM (2 months, 12 days ago)

In addition: I do not pre-seal bags before sterilizing them either.  However, I will seal the bags and roll around the grains, inject LC through the bag then not shake again until 20-30% colonization.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: hamloaf] * 2
    #28734803 - 04/12/24 07:13 AM (2 months, 12 days ago)

Yeah I used to shake jars right after hitting them with lc to spread it around, but it always seems to take significantly longer to get going compared to leaving it for 5-7 days and then shaking.


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OfflinexVadisx
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Re: Cultivation General Discussion [Re: A.k.a]
    #28734837 - 04/12/24 08:05 AM (2 months, 12 days ago)

Huh. I’ve only free poured into bags and then sealed and shook right away. I’ll have several bags coming up to run with LC/SSLC and will try both side by side. For science.


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InvisibleScrimshaw
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Re: Cultivation General Discussion [Re: xVadisx]
    #28734862 - 04/12/24 08:35 AM (2 months, 12 days ago)

Thanks for the info. Yeah I only free pour and I don’t want to switch to syringes at the moment but I can see the benefit of being able to mix before hand.

But just so I’m clear, if you prep millet for example and there is more moisture/wet grains and the bottom and you dump LC in you just let it sit like that until somewhere between recovery and 30% before even distributing it around?

I’m not going to discount anecdotal evidence about shaking LC after inoculation slowing things down, but what’s the scientific basis for it? I use a stir plate while my LC is growing out to periodically break up the myc and I’m sure that’s pretty traumatic. Adding to a bag of grain and shaking up doesn’t seem any different to me on the surface.

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InvisibleInocuole
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Re: Cultivation General Discussion [Re: Scrimshaw] * 1
    #28735817 - 04/13/24 02:26 AM (2 months, 11 days ago)

:splooge:


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: A.k.a] * 2
    #28735960 - 04/13/24 08:00 AM (2 months, 11 days ago)

Quote:

A.k.a said:
Yeah I used to shake jars right after hitting them with lc to spread it around, but it always seems to take significantly longer to get going compared to leaving it for 5-7 days and then shaking.




I'd mix some ground up dog food in my grain jars before sterilizing. Instead of the LI kind of pooling at the bottom, it'd mix with the dog food and stick to the grain. It improved colonization times. Might be worth playing with.

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OfflineDj_karma_magnet
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Re: Cultivation General Discussion [Re: stareatclouds]
    #28743764 - 04/20/24 02:54 PM (2 months, 4 days ago)

Will contam ever give me bunk shrooms? Or will it just give me no shrooms altogether ?

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Offlinerumfor69
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Re: Cultivation General Discussion [Re: Dj_karma_magnet]
    #28743813 - 04/20/24 03:43 PM (2 months, 4 days ago)

Bunk shrooms, no. Possible varying degrees of potency based on environmental stress and genetic development...hard to prove. No shrooms, sometimes depending on the contamination severity but sometimes you get to harvest a bunch still.


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Invisiblestareatclouds
star eat clouds?
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Re: Cultivation General Discussion [Re: Dj_karma_magnet]
    #28743850 - 04/20/24 04:31 PM (2 months, 4 days ago)

Quote:

Dj_karma_magnet said:
Will contam ever give me bunk shrooms? Or will it just give me no shrooms altogether ?




Yes. Sometimes your sub won't make it to flush. Sometimes the shrooms themselves will be affected. For me, that was pretty rare, but it happened a few times where I think I got verticillium. Other times the bacterial sub will cause the fruits to speed up and pump out everything it can initially.

If you mean bunk like no potency, uh, I don't think so. But sometimes a batch can naturally be weaker than you'd expect (less likely with stabilized varieties, I reckon). IIRC, Pasty had an entire flush of PE that was super bunk. What a noob.

Also, it's pretty hard to pinpoint whether it's bacteria specifically or something else causing the issues. It's often going to be a combo of things since bacterial spawn won't result in a healthy enough sub to colonize quickly and defend against bacteria, competing fungi, etc. Even when we call something trichoderma, I don't think we actually know for sure that's what it is. We just see sporulating mold and call it as such.

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OfflineThreads2theSoul
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Re: Cultivation General Discussion [Re: Inocuole]
    #28743903 - 04/20/24 05:39 PM (2 months, 4 days ago)



I was given this LC to use to start this hobby and it produced amazingly.

I'm unable to contact the creator of the pictured LC and am curious to find out how they made it?

Any thoughts?

Were they lucky to get a clean LC from pieces of fruit or did they somehow clean the fruit first?

The posts I could find about cloning were using a syringe or after tearing the stem in half, using a scalpel to put a small piece of inner flesh to agar, then agar to LC. Nothing about slices of fruit like above.

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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: Threads2theSoul] * 7
    #28743929 - 04/20/24 06:08 PM (2 months, 4 days ago)

I'm not entirely sure what I'm looking at. No, they didn't clean the fruit. In the past, people have dropped tissue into liquid (check eat's 9er Tek for a walkthrough), or sometimes straight to grain (azur had pictures of this, I think). But usually it's a piece of tissue to agar like you said.

You can place whole pins on a plate, too. It'll revert into tertiary mycelium:



Here's a tiny sliver of tissue I'm transferring from a fruit I ripped in half:



Some pinning plates that could probably be dumped straight into liquid and blended for fun:



I had a grain spawn bag that I forgot about which fruited some clusters on the top layer. I sliced open the bag, cut off a full fruit, and dropped it into my LI jar (blender blade attachment). I think I wound up with this tub from it:


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OfflineThreads2theSoul
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Re: Cultivation General Discussion [Re: stareatclouds]
    #28754321 - 04/28/24 07:57 PM (1 month, 27 days ago)

Thank you for the info, posts to check out and pics! I appreciate your time and detail, very informative.

I've only worked with quart rye jars, LC and agar, not LI (yet -I don't have the needed equipment). From a round of colonized jars I'll use the best for G2G and put the rest to monos or shoeboxes being sure to put a grain to LC and to agar (as backup to keep it going - I don't do slants yet either). I also gather and store spores as well as clone good looking fruit as I go.

But I'm confused on senescence. When doing grain transfers I've read not to G2G more than 10 cycles (generations I think they called it) saying the fruit would have lost potency and senescence would be an issue by then. That part I get, but when cloning, cloning is not like spores in that senescence does not restart through cloning, only is senescence restarted (probably all wrong terminology...) when starting from scratch with spores. So...

If you are on G2G#4, put a sliver of one of it's resulting fruits to agar, that agar (be it clean) goes to grain, that grain colonized and you want to use one of the jars to do G2G, would that G2G be considered #5? How many times could you G2G that sliver's colonized grain?

And if you had to do multiple agar transfers to get clean mycelium from that sliver, do multiple agar transfers affect senescence?

And... when I use a jar of LC, I'll pour a tiny bit into a new LC jar. Test and use, pouring again a bit into a new LC jar, etc. I've just started doing this. I'm on LC#3. So far so good. What is your opinion on doing this? I figure, IF this process makes it to LC#10 that would be the stopping point for the same reasons for stopping G2G at the 10th generation.

I'm confident with LC, agar and grain work, my process has been working well for me but I know I can't keep doing those cycles forever stretching out the same mycelium over generations and generations... what is your typical work flow?

... sorry... LONG winded...

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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Threads2theSoul]
    #28764301 - 05/06/24 04:11 PM (1 month, 19 days ago)

I lost an ignore, somebody either grew a set or got banned. :notsureif:

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Offlinerumfor69
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 2
    #28764332 - 05/06/24 04:52 PM (1 month, 19 days ago)

Maybe they decided they like you now and wanna be friends, share pet and food pics, go for walks in the park 🤔


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: rumfor69] * 1
    #28764340 - 05/06/24 04:55 PM (1 month, 19 days ago)

Goly, that would be swell.

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Offlinevicepope
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Re: Cultivation General Discussion [Re: Stipe-n Cap] * 1
    #28764803 - 05/06/24 11:50 PM (1 month, 18 days ago)

The more friends the more mushrooms.

Freinds

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