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OnlineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: RockinRobot] * 2
    #28720280 - 03/30/24 01:30 PM (2 months, 29 days ago)

Ime if you don’t give it a few days after full colonization they usually will just eat right through the casing and you have to add more


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LAGM2020

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InvisibleMojaveMyc
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] * 9
    #28720291 - 03/30/24 01:49 PM (2 months, 29 days ago)



Pan Cambo Komodo- it knotted up very well before I got around to casing, so I put it in the martha and let it ride without. Bangin flush but man are they tiny. Sub might be a bit rich

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OfflineNorCal 311
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Re: Official Copelandia/Panaeolus Thread [Re: SGV] * 1
    #28720492 - 03/30/24 04:57 PM (2 months, 29 days ago)

Quote:

SGV said:
Heres a silly question...

I purchased one of those 'straw lovers' kits from a vendor that has the hpoo and straw already pasteurized.  Problem is, its been sitting and waiting while my lazy ass got around to getting some jars done. 

While I know that it doesn't necessarily go 'bad' will I have to re-pasteurize my sub mix?    Ill be less lazy next time and source my hpoo and straw.  (Maybe)




I did the same thing getting ahead of the game and my jars did not colonize, so I kept trying.  It was about 3 months after ordering that I mixed with spawn.  I now have some great cake pans going. This is TTBVI from Gordo


 


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Re: Official Copelandia/Panaeolus Thread [Re: NorCal 311] * 3
    #28721050 - 03/31/24 08:00 AM (2 months, 28 days ago)

I'll just leave this here, Y'all are the best and brightest.

https://www.shroomery.org/forums/showflat.php/Number/28720105

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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: Stipe-n Cap] * 1
    #28723303 - 04/02/24 09:01 AM (2 months, 26 days ago)

Nice trays Norcal, Stipe N cap, looking forward to seeing the results/part 2.

I recently tried sterilizing my sub and casing after I was losing a lot of trays to trich,

Does anyone have any insights as to what might be wrong?  I'm getting a lot of stalling on my trays, a few have fruited decently but others I have a ton of pins but they just won't grow.  This is happening in a Martha setup as well as an open air humidifier in a bath tub set up.  Temps are between 70-80F, Humidity in Martha is at 90% with fae constantly cycling.  CVG+manure sub


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InvisibleNattyOrNice
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 1
    #28723722 - 04/02/24 06:25 PM (2 months, 25 days ago)








Oats mixed with cactus top soil(manure) verm, dried oat grass clippings, and coir.  Didn't really have to shake at all and it colonized really fast!  We were going to just case the jar and fruit invitro...

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OfflineTheLongComma
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Re: Official Copelandia/Panaeolus Thread [Re: NattyOrNice]
    #28724828 - 04/03/24 06:50 PM (2 months, 24 days ago)

:sadanddisappointed:

One P.Cyan spore syringe, one P.Cyan LC, two different sources.

Zero germination on 2% LME Agar, 0.5% LME Agar, BRF pucks, and rye/millet (only the LC).

By contrast, APE had zero problems germinating.

From my understanding, only during fruiting do temperatures need to be 78F-80F and spawn production can be whatever (I keep it 70F-74F).

Spores can be tricky, but I didn't expect this from the LC. I did notice that the P.Cyan LC looked nothing like APE... I had wondered if P.Cyan looks different in an LC.

Any suggestions of how to move forward?



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:badadvice:

Edited by TheLongComma (04/03/24 07:02 PM)

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OfflineBra
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma] * 8
    #28724887 - 04/03/24 07:23 PM (2 months, 24 days ago)

Panaeolus Bisporus Washington



--------------------
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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma]
    #28724960 - 04/03/24 07:55 PM (2 months, 24 days ago)

Quote:

TheLongComma said:
:sadanddisappointed:

One P.Cyan spore syringe, one P.Cyan LC, two different sources.

Zero germination on 2% LME Agar, 0.5% LME Agar, BRF pucks, and rye/millet (only the LC).

By contrast, APE had zero problems germinating.

From my understanding, only during fruiting do temperatures need to be 78F-80F and spawn production can be whatever (I keep it 70F-74F).

Spores can be tricky, but I didn't expect this from the LC. I did notice that the P.Cyan LC looked nothing like APE... I had wondered if P.Cyan looks different in an LC.

Any suggestions of how to move forward?







How long since inoculation? It can take a while but also a lot of people reported issues with pan spore syringes. Doesn't look like there is much mycelium in the pan LC syringe if any. Did you inoculate the rye/millet first without shaking the syringe prior to inoculation. Might have accidentally shot all or most of the mycelium into the grain jar. If you are not getting anything til the jar has finished colonizing then take a few grains and put them to agar or BRF puck before spawning.

Got a photo of the growth on grain?

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OfflineTheLongComma
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28724972 - 04/03/24 08:04 PM (2 months, 24 days ago)

Quote:

Baba Yaga said:
Quote:

TheLongComma said:
:sadanddisappointed:

One P.Cyan spore syringe, one P.Cyan LC, two different sources.

Zero germination on 2% LME Agar, 0.5% LME Agar, BRF pucks, and rye/millet (only the LC).

By contrast, APE had zero problems germinating.

From my understanding, only during fruiting do temperatures need to be 78F-80F and spawn production can be whatever (I keep it 70F-74F).

Spores can be tricky, but I didn't expect this from the LC. I did notice that the P.Cyan LC looked nothing like APE... I had wondered if P.Cyan looks different in an LC.

Any suggestions of how to move forward?







How long since inoculation? It can take a while but also a lot of people reported issues with pan spore syringes. Doesn't look like there is much mycelium in the pan LC syringe if any. Did you inoculate the rye/millet first without shaking the syringe prior to inoculation. Might have accidentally shot all or most of the mycelium into the grain jar. If you are not getting anything til the jar has finished colonizing then take a few grains and put them to agar or BRF puck before spawning.

Got a photo of the growth on grain?




I don't want to break forum rules, but the source of the LC's was recently deemed to be questionable. I've got 2 other P.Cyan LCs and they all look similar to the BVI.

Agar/Grain jars/BRF Pucks were all LC'd on 3/31 in that order. Spore syringes were put to BRF Pucks/Agar 3/15. Zero growth on anything - so nothing to snap a picture of. Syringes were shaken prior to splooging.

I really thought I would've seen something on agar at least :/


Edited by TheLongComma (04/03/24 08:12 PM)

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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma]
    #28724995 - 04/03/24 08:17 PM (2 months, 24 days ago)

The spores should have done something by now since it's almost 3 weeks. I would wait a few more days before giving up on the LC. Photo is a bit small, can you see any mycelium at all in those pan syringes? Ah ok didn't see the 2nd photo, looks like there is some floating in there.

Edited by Baba Yaga (04/03/24 08:20 PM)

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OfflineTheLongComma
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28725000 - 04/03/24 08:22 PM (2 months, 24 days ago)

Quote:

Baba Yaga said:
The spores should have done something by now since it's almost 3 weeks. I would wait a few more days before giving up on the LC. Photo is a bit small, can you see any mycelium at all in those pan syringes? Ah ok didn't see the 2nd photo, looks like there is some floating in there.




Yeah I'm trying to find the balance of resizing 2MB photos to get something with nice resolution. Maybe JPGs are the way to go? (:

There are definitely floaters in the LCs... but they seem like pieces of something. Not puffy like I would expect of myc growing in 3D, if that makes sense.

Honestly, the LCs seem a little nute heavy from their coloration too. I'm wondering if I should just keep the unused syringes set aside and let them grow out a little more?

I want BVI so bad. The other two are Estero and Blue Meanie P.Cyan. I just like wanna join the club, man.


--------------------
:badadvice:

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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma] * 2
    #28725134 - 04/03/24 10:09 PM (2 months, 24 days ago)

Pan myc does look different in LC. I usually don't disturb my LCs much at the beginning and pan mycelium does slow right down after a while and grows denser. It needs to be constantly disturbed to keep expanding. It does look less fluffy and more compact than cube mycelium. Below is a photo of one undisturbed cube LC, an undisturbed pan LC and a pan LC which I shook a couple of times a few weeks ago. Pans are just not fluffing up as much as cubes do at least this is what is happening for most of my pan LCs at a nutrient strength of 0.1% LME.




JPG is working great, this photo is only 130kb after compression.

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Offlinefungalfrontier
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma]
    #28725277 - 04/04/24 02:13 AM (2 months, 24 days ago)




I don't want to break forum rules, but the source of the LC's was recently deemed to be questionable. I've got 2 other P.Cyan LCs and they all look similar to the BVI.

Agar/Grain jars/BRF Pucks were all LC'd on 3/31 in that order. Spore syringes were put to BRF Pucks/Agar 3/15. Zero growth on anything - so nothing to snap a picture of. Syringes were shaken prior to splooging.

I really thought I would've seen something on agar at least :/








If this happens to be the same vendor that I got my pan lc from I'd be a little concerned. That was my initial gripe. The pan lc syringes had no mycelium in them at all. 2 different strains.

I'm finally back to square one , dropped some ttbvi t1 into nutrient broth. Its from a print. Waiting for it to grow out now. 24 hours in so not much to see yet. Got everything prepped and ready to go for it now.


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***🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿***




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Offlinetree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
    #28725369 - 04/04/24 05:39 AM (2 months, 24 days ago)

Quote:

Bra said:
Panaeolus Bisporus Washington






I love how white these are.  I'm also growing a Washington strain and mostly I get dark caps.

Supa put some spores under the scope.  They're pitch black.

I wonder if you have a pheno producing less spore load?  Or not black spores maybe?

My caps turned white once they finished printing too.


--------------------

Edited by tree frog (04/04/24 05:41 AM)

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OfflineAspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: TheLongComma]
    #28725501 - 04/04/24 08:26 AM (2 months, 24 days ago)

Quote:

TheLongComma said:
:sadanddisappointed:

One P.Cyan spore syringe, one P.Cyan LC, two different sources.

Zero germination on 2% LME Agar, 0.5% LME Agar, BRF pucks, and rye/millet (only the LC).

By contrast, APE had zero problems germinating.

From my understanding, only during fruiting do temperatures need to be 78F-80F and spawn production can be whatever (I keep it 70F-74F).

Spores can be tricky, but I didn't expect this from the LC. I did notice that the P.Cyan LC looked nothing like APE... I had wondered if P.Cyan looks different in an LC.

Any suggestions of how to move forward?






Cant speak to the LC, but pan spores seem to degrade much faster than cube spores. My first attempt was at pan cambos and after failing I tried again maybe 3-4 months later but got no germination out of the syringe. You can never be sure how old the syringe is. Maybe its just old.


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Re: Official Copelandia/Panaeolus Thread [Re: AspectOfTheCreator]
    #28725524 - 04/04/24 08:43 AM (2 months, 24 days ago)

Yeah, I've noticed pan spores don't stay good long, too.

Maybe try germinating some on cakes, like in Rev. Myc's thread here?

https://www.shroomery.org/forums/showflat.php/Number/28649340/page/1


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OnlineSmellyhobbitM
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #28725565 - 04/04/24 09:02 AM (2 months, 24 days ago)

Quote:

tree frog said:
Yeah, I've noticed pan spores don't stay good long, too.

Maybe try germinating some on cakes, like in Rev. Myc's thread here?

https://www.shroomery.org/forums/showflat.php/Number/28649340/page/1



:whathesaid:

If you’ve stubborn genes, go to cake or puck. If there’s anything of value left alive in that syringe, you can get it out with a cake/puck


--------------------
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OfflineForestFunGuyGal
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Re: Official Copelandia/Panaeolus Thread [Re: Smellyhobbit]
    #28725730 - 04/04/24 10:44 AM (2 months, 24 days ago)

Ahoy! Can you help me troubleshoot my grow?

What's strange is that my setup used to perform well. Maybe I changed something small without noticing. It's a cooler with a pool of water below, a fan for FAE connected to the outside via HEPA filter, an exhaust, an aquarium heater, and an ultrasonic mister on a timer.

I tune the fan's speed so that a healthy amount of mist can be seen coming out of the exhaust when it's on. In the colder months, I turn the mister off as the aquarium heater creates enough of a temperature difference to supply more then enough humidity (I measure 90% - 100%).

But my fruits look like this. Sparse and malformed. Short, sometimes very thick, like they started growing from under the substrate. Very few of them. :sad:

It's a waylitjim substrate. Cleaned agar to syringe to tub (no grain).

Help??


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OfflineBra
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 2
    #28725756 - 04/04/24 11:07 AM (2 months, 24 days ago)

Quote:

tree frog said:
Quote:

Bra said:
Panaeolus Bisporus Washington







I love how white these are.  I'm also growing a Washington strain and mostly I get dark caps.

Supa put some spores under the scope.  They're pitch black.

I wonder if you have a pheno producing less spore load?  Or not black spores maybe?

My caps turned white once they finished printing too.



I have some exciting news: they have dropped spores! :mushroom2: The prints may not be the thickest, but working on it to improve their quality. Hopefully, can achieve better prints soon. The spores are black.

I believe there is a way to enhance their spore production, although I'm not sure how yet.

I also love their color. It's a beautiful strain. Initially, when they start to grow, they have an olive color. As they begin to drop spores, they develop a bright whitish creamy or elephant bone color. :loveeyes:

I would say they do have a lower spore load compared to some other pans like WCA or Pan Cambodginiensis, but the difference is not drastic. Perhaps they just need some different conditions to improve spore production. Tried to spray them with pure water and it seems that helped a little bit.


--------------------
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