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Offline65PercentOxygen
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Registered: 02/28/23
Posts: 94
Last seen: 2 months, 4 days
Sterilising water for prints * 1
    #28709678 - 03/23/24 08:51 AM (3 months, 2 days ago)

I'm trying to sterilise water for my syringe/prints.

I put boiling water in an egg cup and also a fair bit of boiling water in a saucepan and proceeded to cover the egg cup in foil and then add this to a saucepan of boiling water. I boiled for 5-10 minutes. I'm wondering if this should now be sterile? I'm not sure if the water in the egg cup would of boiled enough to do a good enough job?

Can I have some input as to if this should be sufficient

Thanks in advance

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OfflineSmellyhobbitM
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Re: Sterilising water for prints [Re: 65PercentOxygen] * 1
    #28709682 - 03/23/24 09:09 AM (3 months, 2 days ago)

I’m not sure exactly what that means, but if you need a syringe’s worth of sterile water here’s how you do it:

Boil water for 5-10 minutes
Suck boiling water into empty syringe
Void syringe
Suck boiling water back into syringe
Cap and store

You now have a syringe that is sterile and full of sterile water. Let it cool and flame the needle before use as usual.

Now if you’re trying to sterilize a dish to do the work in, PCing a half pint jar or something like that is good. In theory you should be able to submerge a ramekin or something for a bit. Empty plastic petris are already sterile. They’d work too


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offline65PercentOxygen
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Registered: 02/28/23
Posts: 94
Last seen: 2 months, 4 days
Re: Sterilising water for prints [Re: Smellyhobbit]
    #28709713 - 03/23/24 09:48 AM (3 months, 2 days ago)

Highly logical and makes complete sense thanks!

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OfflineCaptainPuffy
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Registered: 06/08/11
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Re: Sterilising water for prints [Re: Smellyhobbit]
    #28709745 - 03/23/24 10:27 AM (3 months, 2 days ago)

Quote:

Smellyhobbit said:
I’m not sure exactly what that means, but if you need a syringe’s worth of sterile water here’s how you do it:

Boil water for 5-10 minutes
Suck boiling water into empty syringe
Void syringe
Suck boiling water back into syringe
Cap and store

You now have a syringe that is sterile and full of sterile water. Let it cool and flame the needle before use as usual.

Now if you’re trying to sterilize a dish to do the work in, PCing a half pint jar or something like that is good. In theory you should be able to submerge a ramekin or something for a bit. Empty plastic petris are already sterile. They’d work too




Would this be clean enough for agar work?

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OfflineRockinRobot
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Registered: 12/08/22
Posts: 1,245
Last seen: 23 hours, 43 minutes
Re: Sterilising water for prints [Re: CaptainPuffy]
    #28709750 - 03/23/24 10:30 AM (3 months, 2 days ago)

Quote:

CaptainPuffy said:
Quote:

Smellyhobbit said:
I’m not sure exactly what that means, but if you need a syringe’s worth of sterile water here’s how you do it:

Boil water for 5-10 minutes
Suck boiling water into empty syringe
Void syringe
Suck boiling water back into syringe
Cap and store

You now have a syringe that is sterile and full of sterile water. Let it cool and flame the needle before use as usual.

Now if you’re trying to sterilize a dish to do the work in, PCing a half pint jar or something like that is good. In theory you should be able to submerge a ramekin or something for a bit. Empty plastic petris are already sterile. They’d work too




Would this be clean enough for agar work?




I wouldn't personally. I make my sterile syringes when I make my agar. Fill syringe with water, wrap in foil and place in a jar with needle facing up then Pc with the agar. Leave in foil till inside SAB

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OfflineSmellyhobbitM
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Registered: 04/01/22
Posts: 13,169
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Re: Sterilising water for prints [Re: RockinRobot]
    #28709829 - 03/23/24 11:21 AM (3 months, 2 days ago)

I’m not sure what benefit there is to making a syringe for agar worn when you already have a print. It’s 100% usable for PF tek, I’ll say that much. It’s as good as any other syringe you’d get.

I’ve also PC’d jars of distilled water and just sucked into a sterile needle.

Ultimately I don’t see a TON of reasons outside of PF tek to  make spore syringes if you already have a perfectly viable print. You can inoculate hundreds of plates from a single print.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offline10ftTall
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Re: Sterilising water for prints [Re: Smellyhobbit]
    #28709845 - 03/23/24 11:31 AM (3 months, 2 days ago)

Holy fuck, this is a post asking how to "sterilise" water?
This is that hand holding shit Beef was referring to....


--------------------
Looking to absorb as much knowledge as mushroomable

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OfflineSmellyhobbitM
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Registered: 04/01/22
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Re: Sterilising water for prints [Re: 10ftTall] * 4
    #28709852 - 03/23/24 11:35 AM (3 months, 2 days ago)

Look man at some point you gotta learn things. If you don’t know the best way to get a syringe of sterile water there’s no harm in asking.

Let me hold your hand, bb ❤️


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineRockinRobot
Stranger


Registered: 12/08/22
Posts: 1,245
Last seen: 23 hours, 43 minutes
Re: Sterilising water for prints [Re: Smellyhobbit] * 2
    #28709918 - 03/23/24 12:23 PM (3 months, 2 days ago)

Quote:

Smellyhobbit said:
I’m not sure what benefit there is to making a syringe for agar worn when you already have a print. It’s 100% usable for PF tek, I’ll say that much. It’s as good as any other syringe you’d get.

I’ve also PC’d jars of distilled water and just sucked into a sterile needle.

Ultimately I don’t see a TON of reasons outside of PF tek to  make spore syringes if you already have a perfectly viable print. You can inoculate hundreds of plates from a single print.




When I make spore plates from a print I don't make a spore syringe, but I still add a drop of sterile water to help hydrate the spores. This is especially helpful with older spores IMO.

I also use syringes of sterile water for Josex Poke to make LC

Edited by RockinRobot (03/23/24 12:25 PM)

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OfflineSmellyhobbitM
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Re: Sterilising water for prints [Re: RockinRobot] * 1
    #28709951 - 03/23/24 12:45 PM (3 months, 2 days ago)

Absolutely man I do those things too. All great uses. There’s definitely reasons to make syringes or sterile water. For what it’s worth, I’ve done the poke with the boiling water method with 100% success rate.

I was just saying idk why you’d want to use a homemade syringe for agar


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


Extras: Filter Print Post Top
Offlinecybork
Stdt mycology
I'm a teapot Unread Journal User Gallery


Registered: 07/01/23
Posts: 366
Last seen: 15 hours, 19 minutes
Re: Sterilising water for prints [Re: 65PercentOxygen] * 2
    #28709975 - 03/23/24 01:00 PM (3 months, 2 days ago)

No, the water is not considered sterilised after boiling for 5-10 minutes on 100 degrees Celcius.

Maybe good enough / sufficient for your purpose, but it is not considered sterilised when cooked on 100 C for 5-10 minutes.

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