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Phaethon
Stranger


Registered: 07/04/22
Posts: 222
Loc: Colorado
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Re: Ask Quick Questions, Get Quick Answers [Re: Shroomerquest]
#28701919 - 03/17/24 04:00 AM (3 months, 8 days ago) |
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The idea is to use a mason jar as the blender jar - the blender base with blades fits mason jar threads. Some people modify normal mason jar lids to have the blade assembly, but evidently the whole blender base survives pressure cooking. (I don't have an Oster blender, but I've considered getting one for LI - basically what trippytimes was describing, but with plain water in the jar and used to inoculate grain immediately after blending.)
Edited by Phaethon (03/17/24 04:04 AM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
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Last seen: 5 hours, 52 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
#28702006 - 03/17/24 07:29 AM (3 months, 8 days ago) |
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Yeah I went and grabbed one from Walmart. I was less than 30 bucks if I remember. The gasket and blade fits perfectly on a regular mouth jar and then you can buy a bunch of replacement blade kits as well for cheap off Amazon,
https://a.co/d/7AoB23M
You can actually just buy the replacement kit as well and just cut off a screwdriver handle and use it in a drill.
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Shroomerquest



Registered: 04/25/19
Posts: 994
Loc: Clouds
Last seen: 1 month, 9 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
#28702021 - 03/17/24 07:55 AM (3 months, 8 days ago) |
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Yea, this what I’m thinking now
--------------------
 Relax…yOu’re triPping
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,220
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702030 - 03/17/24 08:10 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: Yeah I went and grabbed one from Walmart. I was less than 30 bucks if I remember. The gasket and blade fits perfectly on a regular mouth jar and then you can buy a bunch of replacement blade kits as well for cheap off Amazon,
https://a.co/d/7AoB23M
You can actually just buy the replacement kit as well and just cut off a screwdriver handle and use it in a drill.
Your 1000% right! That's what I do 😂. It works great and you don't have to wait for a piece of agar to make a full LC. once you blend a full plate you can just use it or leave it for a few days and it will be even more ready. I have found this is the quickest way to build a lot of tubs quick.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Re: Ask Quick Questions, Get Quick Answers [Re: trippytimes]
#28702042 - 03/17/24 08:27 AM (3 months, 8 days ago) |
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I’ve used LI to expand LC recently and didn’t care for it. I have yet to try taking LI straight to grain. I like the idea and am going to try it. Waiting a few days is a good pointer! I’m done taking agar to grain for the most part, shit is slow and I also don’t want to make a million LCs lol
You still doing 10cc to a myco quart? How fast does it colonize for you when you wait a few days on the LI?
I wanted to play around with semi solid some more with adding a little agar in the mix. I tried it with LC and it gave me shitty results. I’m wondering if it’s worth trying with LI. I’ve heard of people colonizing jars in just a few days with SSLC
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RockinRobot
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper] 1
#28702044 - 03/17/24 08:31 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: I’ve used LI to expand LC recently and didn’t care for it. I have yet to try taking LI straight to grain. I like the idea and am going to try it. Waiting a few days is a good pointer! I’m done taking agar to grain for the most part, shit is slow and I also don’t want to make a million LCs lol
IMO much easier and safer to just make LC using Josex poke than doing LI.
https://www.shroomery.org/forums/showflat.php/Number/24740168
My issue with LI is that any bacteria rods that may have been inactive sitting on the walls or uncolonized areas can be picked up into the LI once you add sterile water and shake/pulverize it into LI.
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SupaThaRipper
Genetics Hoarder



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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot]
#28702060 - 03/17/24 08:43 AM (3 months, 8 days ago) |
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I know of a commercial grower who expands LC through LI. He does one jar of LI with a full plate. And that one jar of LI expands to 60 jars of LC!
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper] 1
#28702066 - 03/17/24 08:45 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: I know of a commercial grower who expands LC through LI. He does one jar of LI with a full plate. And that one jar of LI expands to 60 jars of LC!
Honestly if you needed to make bulk LC from a good plate that would be the way to do it. Makes sense to me.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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SupaThaRipper
Genetics Hoarder



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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28702071 - 03/17/24 08:48 AM (3 months, 8 days ago) |
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I haven’t had any contamination issues with it but I don’t need THAT much LC of one variety lol. My sole goal right now is creating crosses and not mass production. I want the speed more so than the quantity
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RockinRobot
Stranger


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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702077 - 03/17/24 08:51 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: I haven’t had any contamination issues with it but I don’t need THAT much LC of one variety lol. My sole goal right now is creating crosses and not mass production. I want the speed more so than the quantity
I make LC out of all my genetics. I do it both for speed of colonization and long term storage. I have jars of LC sitting over a year without even being stirred that are still viable. Since I stop growing for long periods, this makes my life much easier. Every time I use a Jar of LC I use it to make a fresh jar and toss the old one once the new one is confirmed clean.
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SupaThaRipper
Genetics Hoarder



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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot]
#28702082 - 03/17/24 08:59 AM (3 months, 8 days ago) |
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Have you ever tried LI personally? I think today I’m going to try to make some semi solid LI. There just has to be a faster way of doing it. It just has to be figured out. I wish the SSLC worked for me like I’ve heard of it working like it does for others. If I could colonize grains in a few days, I’d be ecstatic.
Best speed I got so far are 2.5 lb black cap natalensis grain bags of feed corn colonized in 2 weeks at 76 degrees with LC expanded from LI. Need to get that speed down to a day 🤣
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot]
#28702084 - 03/17/24 09:00 AM (3 months, 8 days ago) |
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Quote:
RockinRobot said:
Quote:
SupaThaRipper said: I haven’t had any contamination issues with it but I don’t need THAT much LC of one variety lol. My sole goal right now is creating crosses and not mass production. I want the speed more so than the quantity
I make LC out of all my genetics. I do it both for speed of colonization and long term storage. I have jars of LC sitting over a year without even being stirred that are still viable. Since I stop growing for long periods, this makes my life much easier. Every time I use a Jar of LC I use it to make a fresh jar and toss the old one once the new one is confirmed clean.
That’s what I’m doing, too. If a culture is worth working on/preserving I make a bottle. They last years on the shelf, according to users here. Great storage method.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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SupaThaRipper
Genetics Hoarder



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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28702088 - 03/17/24 09:05 AM (3 months, 8 days ago) |
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I have 2ml microcentrifuges with LC in them stored. They’re cute when they’re that small. And I can fit 100 in a case lol.
https://a.co/d/0wFIsWI
https://a.co/d/8ayLUgd
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Shroomerquest



Registered: 04/25/19
Posts: 994
Loc: Clouds
Last seen: 1 month, 9 days
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702090 - 03/17/24 09:06 AM (3 months, 8 days ago) |
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What is the difference between LI and LC excuse my ignorance as I assumed it’s the same thing?
--------------------
 Relax…yOu’re triPping
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RockinRobot
Stranger


Registered: 12/08/22
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702091 - 03/17/24 09:06 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: Have you ever tried LI personally? I think today I’m going to try to make some semi solid LI. There just has to be a faster way of doing it. It just has to be figured out. I wish the SSLC worked for me like I’ve heard of it working like it does for others. If I could colonize grains in a few days, I’d be ecstatic.
Best speed I got so far are 2.5 lb black cap natalensis grain bags of feed corn colonized in 2 weeks at 76 degrees with LC expanded from LI. Need to get that speed down to a day 🤣
Never bothered with LI because I learned Josex poke early and also for the reason I previously stated. That being said I use LC and my Jars are ready in 10 days with a shake on day 5. I don't know how much faster you need to be
Edited by RockinRobot (03/17/24 09:07 AM)
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SupaThaRipper
Genetics Hoarder



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Re: Ask Quick Questions, Get Quick Answers [Re: Shroomerquest] 1
#28702094 - 03/17/24 09:09 AM (3 months, 8 days ago) |
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LI (liquid inoculant) is colonized agar pulverized in non nutrient, sterile water.
LC is what you already know
@rockin. With potential contamination aside, i’m going to put it to the test. I’m going to attempt semi solid again with both LI and LC and then non semi solid with both as well. (I couldn’t get semi solid down right last time and hopefully I can this time.) I want to find strictly what’s best for speed on grain.
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Shroomerquest



Registered: 04/25/19
Posts: 994
Loc: Clouds
Last seen: 1 month, 9 days
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702096 - 03/17/24 09:13 AM (3 months, 8 days ago) |
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[Quote:
SupaThaRipper said: LI (liquid inoculant) is colonized agar pulverized in non nutrient, sterile water.
LC is what you already know
Riiight, understand the difference now. Thanks
Im actually currently growing plates of Ps.cyanescens mycelium to do just that, plan is to inoculate some local mulch beds.
--------------------
 Relax…yOu’re triPping
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,220
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Re: Ask Quick Questions, Get Quick Answers [Re: SupaThaRipper]
#28702172 - 03/17/24 10:31 AM (3 months, 8 days ago) |
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Quote:
SupaThaRipper said: I’ve used LI to expand LC recently and didn’t care for it. I have yet to try taking LI straight to grain. I like the idea and am going to try it. Waiting a few days is a good pointer! I’m done taking agar to grain for the most part, shit is slow and I also don’t want to make a million LCs lol
You still doing 10cc to a myco quart? How fast does it colonize for you when you wait a few days on the LI?
I wanted to play around with semi solid some more with adding a little agar in the mix. I tried it with LC and it gave me shitty results. I’m wondering if it’s worth trying with LI. I’ve heard of people colonizing jars in just a few days with SSLC
So I do qt jars and I use 50cc per bag of 3.5-4qt rye.
Waiting that 2-3 days allows the myc to fluff up and start regrowth. Those that say "what about the contams under the plate" never had it happen as I pre pour my plates and wait a week before putting ANYTHING on them.
Which allows me enough time though the whole grow on agar for things to surface.
I personally think my method works best as it's straight agar to LC "LI". Which allows for more evenly dispersed mycelium. I tend to take my syringe and squirt it all over the bag in a circle motion which instead of a agar wedge single point. it's sprays myc all over the place so quicker start. Also that 2-4days resting allows the myc to recover and start on thelat grain quick AF.
If your doing small grows and not crazy bulk you COULD use a small 1/2qt and get same results. Save some for later and store it in fridge for a master slant kinda. So you have some to fall back on. Which you can easy put it back to agar if good culture. Its a huge time saver and the ones who say otherwise are scared or don't have good sterile technique. Contamination is only done when you messed up. 😂
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tossed_salad
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Re: Ask Quick Questions, Get Quick Answers [Re: trippytimes]
#28702230 - 03/17/24 11:03 AM (3 months, 8 days ago) |
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I'm planning on making some blenderless LI later. I just put water in a jar and throw it in the PC for like 15-20 min right?
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alexdahbomb
Reformed Junkie

Registered: 09/27/11
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Re: Ask Quick Questions, Get Quick Answers [Re: tossed_salad]
#28702240 - 03/17/24 11:06 AM (3 months, 8 days ago) |
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Are fuzzy feet/stipe bases genetic?
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