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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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InvisibleDieCommie
Registered: 12/11/03
Posts: 28,149
a little confused on agar, and pressure cooking...
    #2870056 - 07/08/04 09:30 PM (12 years, 8 months ago)

I got petri dishes today, but i am a little confused as how to prepare the agar...

I mixed up the PDYA, per some instructions i found on here....

Do i pressure cook the agar in a quart jar then while hot pour onto plates, and then seal with cling wrap polyethylene?  (My issue with this is will it contam?  Do i need to pour it in a glove box?)

Or...Do i pressure cook the agar in a quart jar for a brief period then pour onto plates and continue pressure cooking? (This seems better to me because it wont be exposed to contams while pouring....should i apply the polyethylene after cooking, when the plate has cooled?)

I was just messin with the cling wrap and the plates....and i cant seem to really get them to cling very well.... fungifun shows a cute little roll bieng pulled taught...is that a portion of a large roll cut off?

Sorry if any of this is stupid and dosent makes sense! :crazy2: THx for any input you have  :thumbup:


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InvisibleYidakiMan
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Registered: 09/29/02
Posts: 2,023
Re: a little confused on agar, and pressure cooking... [Re: DieCommie]
    #2870075 - 07/08/04 09:36 PM (12 years, 8 months ago)

Sterilize for 20-30min at 15 PSI. Take it out and let it cool awhile. In a glove box pour into plates. Allow to cool and harden. Then wrap with cling film. Just cut a 2-3 inch section off the roll.

But this confused me:
>>I was just messin with the cling wrap and the plates....and i cant seem to really get them to cling very well....<<

Did you buy glass petris or did you take the plastic ones out of their sterile bag?


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InvisibleDieCommie
Registered: 12/11/03
Posts: 28,149
Re: a little confused on agar, and pressure cooking... [Re: YidakiMan]
    #2870088 - 07/08/04 09:40 PM (12 years, 8 months ago)

I got glass ones

So i should pour in glovebox....allow to cool and then wrap while in the glovebox? That sounds a little diffucult, but i will try it and see what happens...

Thanks for the quick response man!

edit- i will need to sterilize the petri dishes too....should i wipe them with alcohol while in the glove box, or maybe i should pressure cook them while empty then place in glove box?


Edited by Qubit (07/08/04 09:41 PM)


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InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: a little confused on agar, and pressure cooking... [Re: DieCommie]
    #2870143 - 07/08/04 09:51 PM (12 years, 8 months ago)

Since you have the glass petri dishes I would go with the pc to be sure of 0 contams. I suggest that you wrap them in foil b4 you pc so they wont be exposed to any air during the transfer to the glovebox.
If you can,get yourself a nice ehlynmeyer flask for cooking the agar mix because it has a small opening that makes for easy pouring and less of an enty for contams. The jar will work just fine but the flask is a nice touch.

MYKEY


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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