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OfflineParanoidGuy
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Registered: 01/30/20
Posts: 259
Loc: Europe
Last seen: 3 hours, 10 minutes
Slow agar growth with Pan. Bisporus, Ps. Azurescens, Ps. Cyanescens
    #28689156 - 03/06/24 07:01 PM (10 months, 6 days ago)

I have received some nice sample recently and I started some agar plates with these.

Cubensis samples grow real fast and nice. I don't have any contams and use a flow hood.

The problem is that I get really slow growth with 3 specific species:
  • Pan. Bisporus
  • Ps. Azurescens
  • Ps. Cyanescens


I use MAE agar, with 2% nutrient (10g light MEA for 500ml water), 2% Agar.
Is this a good recipe for them?

I have tried incubating them at 28°C which they did no like at all, now they are back to room temp (21°C) and colonizing so slowly. In two week, the growth is smaller than a 1€ coin.

The petri dishes are glass petris wrapped with horticultural parafilm.

Any advice would be appreciated.

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Invisiblestroopwafelman
I'm a teapot

Registered: 03/21/23
Posts: 386
Re: Slow agar growth with Pan. Bisporus, Ps. Azurescens, Ps. Cyanescens [Re: ParanoidGuy]
    #28689358 - 03/06/24 09:34 PM (10 months, 6 days ago)

2% is too high for Pans. The suggested nutrient ratio is 0.5-1%

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