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OfflineMK-801
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Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting
    #28688372 - 03/06/24 07:11 AM (3 months, 19 days ago)

I allowed the mycelium to colonise in the dark at 23-27C, that took a couple of weeks however they began fruiting in the centre, even though it wasn't fully colonised and in darkness. So I decided to attempt fruiting and dropped the temps to 20-23C, allowed airflow and started misting and got them in the light during the day.



Had contamination from what I think was cobweb mould, so I carefully covered it with tissue soaked in rubbing alcohol and removed the whole section of substrate. I've had the tub lid shut, fanning a couple of times a day. I was hoping that more of the tiny pinheads would start fruiting, but the ones that have haven't grown for about a week (fruiting been going about 10 days).

But the few in the centre (they were the bodies that started in the dark) are still growing, especially that big one which is almost looking mature to me? I'm beginning to think that the mould has contaminated other sections of the substrate, which is preventing growth (first grow so I'm not quite sure what it's meant to look like. Or I'm misting too much/too little or not getting enough fresh air exchange?)



Any tips to help the rest start fruiting? Maybe my temps/humidity are off... Or perhaps this one might be a lost cause and even dangerous to continue with, could even be bacterial contam that I'm not experienced enough to spot...

I'm thinking they need more air exchange, so I put a small fan pointing at the tub for an hour a day, hopefully no more contamination will get in because of the cotton barriers. But yes, any tips from an experienced grower would be super helpful.

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OfflineSmellyhobbitM
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688379 - 03/06/24 07:30 AM (3 months, 19 days ago)

Well you’ve got a ton to learn. Pretty impressive that you got to the point of fruits in general.

You need to read the link in my sig “a guide for new growers” in its entirety. You’ve done just about everything here wrong.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineMK-801
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: Smellyhobbit]
    #28688383 - 03/06/24 07:36 AM (3 months, 19 days ago)

Thanks friend, I have read many guides/watched videos but not the one you have linked, will read it now. So you think it's a lost cause then?

One thing I forgot to mention is I was having trouble keeping the temps low and consistent enough, due to it being 35+C where I live every day and having shitty AC. That's one reason why the temp fluctuations are high, and also I had a couple of power outages lasting 2-3 hours where the temp rose to 35C.

I'll pop back into this thread once I've read the guide, cheers.

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InvisibleDaeda1us
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801] * 1
    #28688384 - 03/06/24 07:38 AM (3 months, 19 days ago)

lots to unpack here. read the guide in my signature.
  • light is beneficial at all stages of growth, 12-12 cycle. most important when pinning
  • your temperature is fine. fluctuating temps will not trigger fruiting like other species. if you're comfortable, so are your cubes.
  • spraying rubbing alcohol or removing a chunk of substrate to fight contamination is a bad idea. i get that you want to try and save it. focus on CLEAN SPAWN first.
  • if it was contaminated, it probably still is.
  • you have a well built monotub. if you properly hydrate your substrate, you shouldn't need to mist. maybe a few times if your FAE isn't dialed right.
  • if you loosen and tighten your polyfill, you will adjust your FAE. don't point a fan directly at the tub.


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New or Returning Grower? Start here

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OfflineMK-801
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688401 - 03/06/24 08:06 AM (3 months, 19 days ago)

Really great guide, I've read most of it and most of the relevant links to other threads, thank you for that.

I should have mentioned, I used pre-prepared spawn jars (cakes), there's no guarantee they were sterile (I know, I know, spore syringes aren't available to me unfortunately). I was careful with contam but I don't have a laminar flow hood or anything, just tried to be as sterile as possible while preparing (no moving air, gloves/mask etc).

Mistake 1 was not preparing the bulk substrate carefully enough, and possibly should have used a liner (although user BeefSupreme
jr mentions they don't, I didn't prepare it as carefully and it wasn't smooth).

Mistake 2, not letting the coco coir cool enough after boiling water sterilisation before mixing with the spawn, although I believe the water content was OK (squeezing made just a little water escape). The temp of the coco coir was around 35C which dropped to 25 in a few hours.

Mistake 3, was thinking that the myc had started fruiting when actually it needed far longer to colonise and pin properly, I should have kept it in the dark for at least another week possibly.

Mistake 4, didn't check for surface moisture before attempting fruiting.

Mistake 5, too much airflow too early in the process (not too sure about this)

I'm sure there's a lot more I did wrong, I'm fine with criticism. The temperature fluctuations could be solved if I get the AC replaced or fixed, not exactly a mistake but an unfortunate problem.

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OfflineMK-801
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688404 - 03/06/24 08:12 AM (3 months, 19 days ago)

Thank you for the tips, I will read the guide you linked too.
I believe I was misting too much, and I imagine the contamination was affecting the growth early on too.

One question (may be in the guide), how do you monitor the FAE, simply by observation? These are probably the trickiest things, making sure the humidity and FAE are optimal. I've looked at many pictures but they can look very different to what's going on in my own tub.

Edit, interesting what you say about 12/12 light being beneficial in all stages, I've mainly heard that darkness is better for colonisation.

Edited by MK-801 (03/06/24 08:14 AM)

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OfflineRockinRobot
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688413 - 03/06/24 08:22 AM (3 months, 19 days ago)

You monitor FAE by observing surface conditions. There should be tiny beads forming on the mycelium. If beads are too big need more air.

To my knowledge, the only mushrooms that require a dark period are Cordyceps on the medicinal side.

Dark periods and reduced FAE are old TEK. These days most of us go directly to fruiting conditions as soon as we spawn.

Misting was likely NOT the cause of your issues. Contamination is almost always caused by bad spawn.

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OfflineMK-801
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: RockinRobot]
    #28688420 - 03/06/24 08:31 AM (3 months, 19 days ago)

Very interesting, I must have been looking at old teks as you say, was told by some people irl to keep the tub in the dark too.

So also I should allow FAE from the very start, so that means not taping up the holes at all, and just using cotton foam or similar to plug them?

Cheers for the tips, I'm learning some great stuff I should have posted here earlier.

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InvisibleDaeda1us
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688422 - 03/06/24 08:31 AM (3 months, 19 days ago)

monitoring FAE is done through observation of the surface conditions. you should have beads of moisture on the surface at full colonization. if you hydrated your sub correctly, it should do this on its own.

drying up too fast? too much FAE. standing water? not enough. humidity takes care of itself as the surface conditions create a micro climate suitable for pins to form.

i've always read here that 12-12 is fine, or that total darkness is antiquated. things like Shiitake have observable differences when growing in light vs dark on agar.


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New or Returning Grower? Start here

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OfflineMK-801
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Registered: 03/06/24
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: Daeda1us]
    #28688455 - 03/06/24 09:09 AM (3 months, 19 days ago)

Great info, yes I believe my substrate may have been too dry during the colonisation period even though the holes were taped up. Perhaps I lost too much water during preparation/mixing of the spawn and substrate.

Something I forgot to mention, I have a shit ton of sidepins, I assumed this was because I didn't use a liner and possibly because I didn't compress the substrate enough into the tub.

So do you guys think this current project is a lost cause? I'll keep it going for a while but it seems that the contamination and my poor technique has made it fail.

I have some more spawn jars, I will upload photos tomorrow in a new thread as one of them in particular does look a bit weird. I have them in the fridge as it's so hot here, but I read on this forum that they should be fine for a few months in there.

Cheers

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OfflineRockinRobot
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801] * 1
    #28688460 - 03/06/24 09:12 AM (3 months, 19 days ago)

You can't fix the current grow. Just let it go and harvest what ya can from it till it completely contams.

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OfflineMK-801
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: RockinRobot]
    #28688482 - 03/06/24 09:25 AM (3 months, 19 days ago)

Ok I got it, so I'll keep it in the current environment for the moment (20-23C, reasonably high humidity inside the tub, 12/12 light) and hope that my fungus can fight the bad ones for at least a little while.

How close do you think those bigger ones are to maturity? Anything I can do after the first harvest to get another flush, I guess balancing the misting and FAE to optimal conditions?

Sorry for all the noob questions, I have read the majority of the guides you guys linked and learnt a lot. I might start a new grow tomorrow with some Melmac spawn as that spawn jar looks to be decent to me, and I have another monotub.

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OfflineSmellyhobbitM
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: MK-801]
    #28688489 - 03/06/24 09:30 AM (3 months, 19 days ago)

You need to pick those fruits and toss the tub. It’s not going to recover and give you anything more, unfortunately.

When you see mold, it’s already in the entire substrate. It’s not just in that one spot. By cutting a huge chunk of the grow out, all you did was weaken the cube mycelium. If it were healthy, it would have a good chance to recover. It is not healthy, and now it is also wounded.

Pick your mushrooms, toss the tub, and start a new project. Make everything yourself. Don’t buy premade cultivation products. They are a waste of your funds and prone to failure.

You definitely can do this yourself with a lot of reading a little practice, so don’t waste your time on something that’s already failed.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineMK-801
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Registered: 03/06/24
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Re: Albino Chodewaves acting strange, first grow and having problems with contamination and lack of fruiting [Re: Smellyhobbit]
    #28688506 - 03/06/24 09:50 AM (3 months, 19 days ago)

Thanks for the clarification about the mould, it was what I suspected however the current fruits are growing larger every day. But yeah I don't really want to harvest mouldy shrooms.

Unfortunately I can't access spore syringes AFAIK due to my location, so I will attempt more grows with the spawn jars. May as well give it a go seeing as I have them.

I will look further into accessing spore syringes so I can make my own spawn in the near future.

Thanks for the valuable info, I'm certainly not giving up yet. However I'll upload some photos of the spawn jars in a future thread to get some expert eyes on them, just to give me an idea if they are already contaminated.

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