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Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale

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OfflinePsychedelicGator
Lost in the Swamp

Registered: 01/18/24
Posts: 90
Last seen: 1 month, 9 days
Trying to force a Subtropicalis plate pin
    #28687918 - 03/05/24 07:25 PM (10 months, 7 days ago)

Hi all, question for the group. I've got a MSS of Subtropicalis and I'd like to hunt down a variant that does well in monotubs. I poured some agar in sterile, breathable petri dishes and streaked them out. They colonized well and I was hoping to find a pin any day now, but unfortunately all of the agar started to dry out as of today. So should I abandon these plates and try again with parafilm? Or maybe let them go and see if I still get a pin with time? Or is this entire endeavor dumb and I should just buy some LC? :smirk: thanks for any advice!

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Offline2Lazy2Bee
Recovering Uncle Ben


Registered: 02/19/24
Posts: 62
Loc: Las Vegas
Last seen: 2 months, 4 days
Re: Trying to force a Subtropicalis plate pin [Re: PsychedelicGator]
    #28687988 - 03/05/24 08:03 PM (10 months, 7 days ago)

I've never not parafilmed my petri's tbh.

How long did it take for them to dry out? Were they stored in a fridge too?


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OfflineFungus Gnat
 User Gallery

Registered: 11/08/22
Posts: 321
Loc: PNW
Last seen: 3 hours, 12 minutes
Re: Trying to force a Subtropicalis plate pin [Re: 2Lazy2Bee]
    #28688007 - 03/05/24 08:11 PM (10 months, 7 days ago)

My subtrops took a looooong ass time to pin on agar but did eventually--probably 9 months or so. Definitely need to be wrapped if they're gonna sit that long.

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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany
Trusted Cultivator
Re: Trying to force a Subtropicalis plate pin [Re: 2Lazy2Bee]
    #28688010 - 03/05/24 08:12 PM (10 months, 7 days ago)

Poured too thin?
Avoid temp swings.

    :cookiemonster:


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