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OfflineDaddy_Diz
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Registered: 03/08/21
Posts: 15
Last seen: 3 months, 20 days
Newbie grower here, is this batch ruined? Discolored spots....
    #28684974 - 03/03/24 11:15 AM (3 months, 22 days ago)




Hi all, this is only my second attempt at growing, but my last try was a few years ago. My first try went perfectly, but I might have blown it this time. These pics are taken before adding the layer of coco coir to the tub, following a monotub process I watched online. There's this dark green growth in one area and lots of yellowish spots across the mycelium.  Also can't tell if maybe I'm looking at cobweb mold? I didn't have any of this happen last time so I'm not sure what I'm looking at. Any help would be greatly appreciated! Thanks.

Edited by Daddy_Diz (03/03/24 12:03 PM)

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OfflineHappinessStan
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Registered: 10/10/12
Posts: 1,777
Loc: Worcester, UK
Last seen: 10 days, 5 hours
Re: Newbie grower here, is this batch ruined? Discolored spots.... [Re: Daddy_Diz]
    #28685009 - 03/03/24 11:42 AM (3 months, 22 days ago)

It's mold, not sure which mold but, it ain't cobweb.
The yellow spots are metabolites, usually a sign the mycelium is fighting off contams. You likely had bacterial spawn which allowed an airborne mold contam to take hold.
Was this grow from a kit of premade spawn/all in one bag? How did you inoculate?
Also, don't name vendors in your posts.


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OfflineDaddy_Diz
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Registered: 03/08/21
Posts: 15
Last seen: 3 months, 20 days
Re: Newbie grower here, is this batch ruined? Discolored spots.... [Re: HappinessStan]
    #28685036 - 03/03/24 12:05 PM (3 months, 22 days ago)

Oops thanks, I edited it to remove the name.

This was done in a grain spawn bag with an injection port, inoculated with a premade liquid culture syringe.

I was so careful to stay sterile through the process. I did not flame sterilize my needle though, I only wiped it with isopropyl alcohol. Not sure if that was my downfall.

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OfflineHappinessStan
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Registered: 10/10/12
Posts: 1,777
Loc: Worcester, UK
Last seen: 10 days, 5 hours
Re: Newbie grower here, is this batch ruined? Discolored spots.... [Re: Daddy_Diz]
    #28685044 - 03/03/24 12:12 PM (3 months, 22 days ago)

Quote:

Daddy_Diz said:
Oops thanks, I edited it to remove the name.

This was done in a grain spawn bag with an injection port, inoculated with a premade liquid culture syringe.

I was so careful to stay sterile through the process. I did not flame sterilize my needle though, I only wiped it with isopropyl alcohol. Not sure if that was my downfall.



If the premade liquid culture was clean, which you wouldn't know unless you tested it on agar, then the contam was almost definitely caused by not flame sterilising; isopropyl alcohol only sanitises, it doesn't sterilise.
Pre made anything is risky business and not guaranteed to give you success.
If you don't have a pressure cooker to make your own grain spawn, agar plates, and liquid culture ,then PFtek is better suited to your needs.


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OfflineDaddy_Diz
Stranger

Registered: 03/08/21
Posts: 15
Last seen: 3 months, 20 days
Re: Newbie grower here, is this batch ruined? Discolored spots.... [Re: HappinessStan]
    #28685075 - 03/03/24 12:44 PM (3 months, 22 days ago)

Okay, thank you for that info. I still have half of the same syringe left. I'm going to try again and flame sterilize this time. I don't know what PFtek means to be quite honest with you, haha. All I know how to do so far is this monotub method.

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