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OfflineRonnyBoy
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Registered: 03/03/24
Posts: 3
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Last seen: 1 month, 26 days
Grain-water agar plates not even growing mold?
    #28684677 - 03/03/24 04:42 AM (10 months, 9 days ago)

Hey Guys I made some grain water agar a week ago to germ some spores. After a while of seeing absolutely nothing, I opened a plate and contaminated it on purpose. I put some dirt on it, and even a dead mosquito lol, and waved it around outside. It's been like 5 days since contaminating the plate on purpose and there's discoloured bits under the dirt and it's clearly got SOME kind of contam, but there's not any mold growing on it. Makes me think it's somehow got anti-fungal properties 🤔?

The contam just looks like discoloured agar, it doesn't look slimy like bacteria or slime mold either. And there's clearly no moldy mycelium on it at all.


Here's how I made the agar:

1. Cook some rice for dinner in  more water than required.
2. Before all the water boils away, grab a cup (250ml) of the boiling water from the pot.
3. Add 4g of agar-agar powder, PC, and pour into ketchup containers.


Maybe it's got too much nutes? The rice water was quite cloudy when I pulled it out of the pot, clearly quite starchy. I can't make MEA or PDA where I live because I don't have access to malt extract or dextrose. Maybe the overabundance of nutrients favours non-fungal microorganisms?

For grain water agar is it enough to just take water from the unboiled grain soak? Feels like there wouldn't be enough nutrients.

Edited by RonnyBoy (03/03/24 04:45 AM)

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