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Amazon Shop: ½ Pint Jars, 10cc Syringe, Pressure Cooker, Wild Bird Seed

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Offlinewhiteadonis
Master of theUniverse
Registered: 06/22/04
Posts: 5
Last seen: 13 years, 1 month
Cake Cannibalism
    #2868350 - 07/08/04 11:49 AM (13 years, 1 month ago)

i have one 100% colonized cake and 5 empty jars. i want to cut the colonized cake up and use it to colonize 5 new jars. how should i do this? how can i fit a big chunk of colonized cake into sterilized jars? i was thinking of filling the jars halfway sterilizing them then put the chunk in and packing the rest with sterilized substrate. sound like a good idea? anyone try this? any suggestions?

peace  :rasta:


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OfflineTantalus
Beyonddescription.
Registered: 06/05/03
Posts: 747
Last seen: 11 years, 11 months
Re: Cake Cannibalism [Re: whiteadonis]
    #2868428 - 07/08/04 12:27 PM (13 years, 1 month ago)

I take it this is a rice cake? Your best best would be to cut a chunk off & put it in a sterile container with some glass shards to make a mycelium syringe and fruit what you don't use to give yourself some experience with the process.

Be careful with sterility, get a pressure cooker if you don't already have one.


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Offlinewhiteadonis
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Registered: 06/22/04
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Re: Cake Cannibalism [Re: Tantalus]
    #2868474 - 07/08/04 12:44 PM (13 years, 1 month ago)

Mycelium syringe? also by "fruit what you dont use" you mean put the rest of the cake in the terrarium right?


Edited by whiteadonis (07/08/04 12:59 PM)


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Offlinedjd586
Underpants Gnome

Registered: 02/03/03
Posts: 1,655
Last seen: 7 years, 5 months
Re: Cake Cannibalism [Re: whiteadonis]
    #2868531 - 07/08/04 01:08 PM (13 years, 1 month ago)

With a sterile knife, cut off a piece of colonized cake. Soak the piece in a water/bleech solution for 6 hours. 1 part bleech to 200 parts water.

Take a jar, sterilize it. Take some water and add some honey. 50/1 water to honey. Put the solution in the jar, sterilze the jar with the mixture in it. You can do this by placing the jar in boiling water for 60 minutes. Let the jar cool. With a sterile knife, cut off a piece of colonized cake. Soak the piece in a water/bleech solution for 6 hours. 1 part bleech to 200 parts water.

Take the cake piece out of the water bleech solution. Open the lid to the honey/water jar and quikcly place the piece of the cake into it. Seal the lid tight. Set the jar aside for 5 days.

After five days take the jar and puncture a hole in the top of the lid. Before you do this, shake the jar up really good. Take a sterile syringe. Insert the syringe into the hole you punctured. Draw out 10cc of fluid. You now have a mycelium syringe. It will be more then enough to innoculate and coloinze 5 more jars.


--------------------

Phase 1... collect underpants... phase 2...??? ... Phase 3 - PROFIT!


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Offlinewhiteadonis
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Registered: 06/22/04
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Re: Cake Cannibalism [Re: djd586]
    #2868606 - 07/08/04 01:35 PM (13 years, 1 month ago)

will a cake innoculated with mycelium colonize faster? :syringe:


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OfflineJuan_del_Pueblo
Mr.Papashroom
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Registered: 06/26/04
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Re: Cake Cannibalism [Re: whiteadonis]
    #2868630 - 07/08/04 01:43 PM (13 years, 1 month ago)

I've done what your talking about with great success. I use a 4 to 1 ratio. Ziplock gallon bags are great with a piece of polyfil. Pack it lightly, but somewhat sculptured(for when u dunk).

I've made myc water, which is great stuff but only with popcorn. The shite is like lightnin. Takes 8-12 days 100% col w/popcorn.


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Offlineddaedalus
poser

Registered: 02/01/04
Posts: 48
Loc: Canadia
Last seen: 12 years, 7 months
Re: Cake Cannibalism [Re: whiteadonis]
    #2869249 - 07/08/04 05:20 PM (13 years, 1 month ago)

Quote:

will a cake innoculated with mycelium colonize faster?





Yes, doing this removes the time needed to germinate the spores.

Do what djd568 suggested, but one alternative would be to leave out the honey from the sterile jar. Just dissove the chunk of myc and inject away. This helps in two ways:

1. It cuts the risk of contamination significantly since there is nothing for the bacteria/mold to feed on.

2. It also saves time because you can skip right from the preperation of the jar to the innoculation. (ie. no waiting 3-5 days the myc to colinize the honey)


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OfflineQandA
wanderer

Registered: 05/19/04
Posts: 352
Last seen: 13 years, 6 days
Re: Cake Cannibalism [Re: ddaedalus]
    #2869929 - 07/08/04 08:48 PM (13 years, 1 month ago)

Quote:

ddaedalus said:
Quote:

will a cake innoculated with mycelium colonize faster?





Yes, doing this removes the time needed to germinate the spores.

Do what djd568 suggested, but one alternative would be to leave out the honey from the sterile jar. Just dissove the chunk of myc and inject away. This helps in two ways:

1. It cuts the risk of contamination significantly since there is nothing for the bacteria/mold to feed on.

2. It also saves time because you can skip right from the preperation of the jar to the innoculation. (ie. no waiting 3-5 days the myc to colinize the honey)


You only want to put them in straight water if you are going to innoculate immediately. otherwise the mycelium will die.


--------------------
I'm not crazy...crazy.


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Offlinehydr0420
Psycopahtic Ryda

Folding@home Statistics
Registered: 05/31/04
Posts: 328
Last seen: 5 years, 4 months
Re: Cake Cannibalism [Re: QandA]
    #2870793 - 07/09/04 12:29 AM (13 years, 1 month ago)

I did this a while back. I used 1 pf cake to innoc 6 1qt WBS jars. I just cleaned a room, put on gloves, Prepared my wbs, PC'd etc. and then opened the pf cake, cut it into 6ths and put them in the WBS jars. They colonized within a week. Since that worked so well, i then took one of those, and innoulated 12 more WBS qts. Those grew fine, no contams yet. Grain 2 Grain is fun. I think I just got lucky with no contams though.
-dr0


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OfflineBlue_Apocalypse
Central NervousSystem

Registered: 07/08/04
Posts: 108
Loc: Illinois
Last seen: 13 years, 29 days
Re: Cake Cannibalism [Re: whiteadonis]
    #2870852 - 07/09/04 12:39 AM (13 years, 1 month ago)

What you are talking about is grain transfer or grain-to-grain transfer. Its usually done with grain by sterilizing a jar of grain, scooping some myc. covered grain with a sterile tool and dumping it in the presterilized jar. The contents are then shaken and put in an 82F incubator to colonize. As always, aseptic technique is required.


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Pressure Cooker, Wild Bird Seed

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