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OfflineTakeatrip
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Registered: 09/10/23
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Last seen: 3 months, 14 days
How can I fix potential contaminated water in my spore syringe?
    #28679394 - 02/28/24 09:50 AM (10 months, 13 days ago)

The reason I ask this is I am having zero results trying to germinate three strains I have and it’s getting silly.
If I streak one or two drops onto some agar plates in a sterile environment (my SAB) all I get is a whitish substance which just gets wetter but never seems to grow any mould or anything then after a few weeks I throw them in the bin and repeat only to get the same result. So, I bought some different spores from another vendor and the same again, no mould, no mycelium, just get a creamy white wet looking mess on the plates. WTF?
Odd thing is I did get success a while ago just dropping some spores onto the agar and leaving it but that strain didn’t give me any hassle.
I have even tried making BRF cakes and successfully inoculating those and then transferring some of that mycelium to agar and I get the same wet looking weird stuff on the plate.
What the heck is causing this and why didn’t it do it with all my syringes? Is there a way I can try to get these spores to germinate any other way? Does this happen to any of you?
Maybe I should try getting hold of spore prints and making my own sterile water and adding some spores to that and making my own syringes? I buy pre poured sterile plates as I don’t have the space or equipment to pour agar successfully here, I have wasted too much on that already.
I don’t want to give up, I am just hitting a point where something has to be changed in order to ascertain what is causing this issue.
Any help would be massively appreciated 👌🏻

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OfflineDERRAYLD
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Registered: 05/13/02
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip] * 1
    #28679397 - 02/28/24 09:51 AM (10 months, 13 days ago)

That slime, white goo is bacterial contamination.

Are you flaming the tip of the needle before innoculating?

Chances are it's a dirty syringe but also your technique at fault.

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Offline2Lazy2Bee
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Registered: 02/19/24
Posts: 62
Loc: Las Vegas
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip]
    #28679441 - 02/28/24 10:30 AM (10 months, 13 days ago)

Quote:


Maybe I should try getting hold of spore prints and making my own sterile water and adding some spores to that and making my own syringes? I buy pre poured sterile plates as I don’t have the space or equipment to pour agar successfully here, I have wasted too much on that already.






That's a good idea as now you'll know the condition of the syringes for sure.

But--- Spores in general are assumed to be vectors of contamination because the fruiting chamber has been in contact with open air. So some level of contaminate from the spore syringe / print is normal.

Bro! You should also just pour your own agar. Get a sleeve of condiment cups, agar agar, light malt extract, an old glass bottle, and microwave it dude!

It's not ideal--- but it works and it sure as hell beats spending 20+ dollars everytime you want 10-20 petris.

Good luck.


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2 in the Meme, 1 in the Dream.





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OfflineAspectOfTheCreator
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Re: How can I fix potential contaminated water in my spore syringe? [Re: 2Lazy2Bee]
    #28679559 - 02/28/24 11:38 AM (10 months, 13 days ago)

Ime syringes do not work well with agar. Syringes are for pf tek. Spore prints for agar.


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OfflineTakeatrip
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Registered: 09/10/23
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Last seen: 3 months, 14 days
Re: How can I fix potential contaminated water in my spore syringe? [Re: AspectOfTheCreator]
    #28680435 - 02/28/24 09:50 PM (10 months, 13 days ago)

Quote:

AspectOfTheCreator said:
Ime syringes do not work well with agar. Syringes are for pf tek. Spore prints for agar.



You can’t get LME syringes, do you mean syringes don’t work well with agar? Spore prints are expensive. Just found some prints on a UK website and they want £39 per spore print.
It’s pricey but at least I should get the results I am hoping for.

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OfflineDERRAYLD
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip]
    #28680491 - 02/28/24 10:55 PM (10 months, 12 days ago)

No reason to go for a more expensive alternative,  if you can get cheap spore syringes then use them.

Just a drop streaked on a plate is fine.
The spores will be hydrated in a syringe as well.

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OfflineDERRAYLD
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Re: How can I fix potential contaminated water in my spore syringe? [Re: DERRAYLD]
    #28680495 - 02/28/24 10:58 PM (10 months, 12 days ago)

Quote:

DERRAYLD said:
That slime, white goo is bacterial contamination.

Are you flaming the tip of the needle before innoculating?

Chances are it's a dirty syringe but also your technique at fault.




?

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OfflineSkropi
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Registered: 11/01/23
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip]
    #28680502 - 02/28/24 11:11 PM (10 months, 12 days ago)

A SAB is not a sterile environment, far from it, it isn't even sanitized, and it doesn't need to be. Working in a SAB requires discipline and experience, it is not hard, but it isn't easy either. The whole point is that the air is still inside, so contams cannot easily fall where you don't want them too. Just some adjustments on how you move in the SAB, how you position your hands etc, will make all the difference. My first plates also got slimy, and I now I can confidently work on agar, with the only difference being, the way I move and position my hands and tools inside it. You also don't need the perfect culture from the get go, just enough to be able to transfer, and start cleaning it up, but to get there, it is a matter of technique mostly.


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Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

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OfflineAspectOfTheCreator
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip]
    #28680510 - 02/28/24 11:18 PM (10 months, 12 days ago)

IME: In my experience


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OfflineLogicaL ChaosM
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Re: How can I fix potential contaminated water in my spore syringe? [Re: Takeatrip] * 1
    #28680533 - 02/29/24 12:27 AM (10 months, 12 days ago)

U cant reprocess a spore syringe to make it clean. Your best best is to shake the syringe up real good then drop a drop or two onto an agar plate. Or just do PF Tek with the syringe.


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OfflineTakeatrip
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Registered: 09/10/23
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Re: How can I fix potential contaminated water in my spore syringe? [Re: LogicaL Chaos]
    #28680954 - 02/29/24 10:20 AM (10 months, 12 days ago)

Quote:

LogicaL Chaos said:
U cant reprocess a spore syringe to make it clean. Your best best is to shake the syringe up real good then drop a drop or two onto an agar plate. Or just do PF Tek with the syringe.



I will do this and show you my results. I repeatedly get the same issue again and again, although I have had success with spore syringes on agar before.

Edited by Takeatrip (02/29/24 09:42 PM)

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