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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
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Supa’s LIVE/UNCUT H2O2 experiments! 10
#28677833 - 02/27/24 07:36 AM (3 months, 27 days ago) |
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I’ve been experimenting with the potential benefits of H2O2. This subject is highly debated and most scientific studies would push you away from H2O2. Currently I have no intentions of ever trying to save a grow with H2O2. This thread will be more so for studying preventative measures. I’ll experiment and post live and uncut results to give everyone as clear of results as possible. ALL FAILURES WILL BE POSTED as I go along.


Don’t expect this thread to be organized or anything like that as I’ll have multiple experiments going at once. All hate comments welcomed 🤣 Now that being said, experimenting with H2O2 is not something currently recommended to new growers (I’m hoping to eventually change that) In the meantime, follow a tried and trusted tek to the T and if you come across contaminations and are unclear about how to proceed, try asking in the quick questions thread or use the search function to see if its already been answered recently. 🍻
DO NOT DO WHAT I DO IN THIS THREAD!! THESE ARE UNSANITARY PRACTICES AND DOING SO WILL SURELY LEAVE YOU WITH NOTHING BUT FAILURES!
Let’s get into it.
2-26-24
While harvesting a fruit I intended to clone, I dropped it on the floor, went to pick it up with my toes and accidentally kicked it across the room 🤦♂️ clumsy me!

Finally, I managed to snatch the little bastard!

I sprayed the fruit generously with H2O2 and placed it in front of my ffu to let it dry. Then took a tissue sample to clone from the exterior of the fruit.

I also cloned from the interior, to ensure I don’t lose this clone all together.
2-28-24
The exterior clone tissue plate has zero signs so far of bacteria or any other contamination. The mycelium has started to show itself.


3-1-24
The exterior clone plate is doing well. No signs of contamination still as the mycelium continues to grow. This will be the last update for a while until significant growth has occurred or until a contamination appears. ( don’t want to bog this post down with too many pictures)

3-3-24 (I lied lol)
Here’s another update of the exterior clone plate again today

3-13-24


Experiment concluded!
Next Experiment
1-21-24
I have a grow bag loaded with the notorious trich! I picked various fruits with some directly above and gradually leading away, with one as far as way from the trich as possible. All fruits were generously sprayed with H2O2 and left to dry in front of the ffu.



I tried finding these swabs briefly yesterday to take to agar but couldn’t 🤦♂️ no worries though, I will today!
2-28-24
I didn’t find the swabs yesterday but I found them today. I took the 2 most hazardous swabs from fruits in direct contact with the trich. The plates labeled “h2o2x2” means they were sprayed again once today during inoculation of the agar. One plate had the swab sprayed before streaking. One had the plate itself sprayed after streaking. The plates labeled “h2o2x1” means they only had the initial spray of the fruits during harvesting and swabbing. No additional sprays were done.

3-1-24
No signs of growth from any of the four plates (contaminants or mycelium.) will hold of on picture for now.
3-3-24
Both plates with only the initial fruits sprayed are clearly contaminated with what I’m sure will be trich.



However, the plates that were treated twice with h2o2 are doing well today! Tops removed for clearer pictures. The plate with several germination had the swab sprayed. I pulled potential monos from this plate. The plate with only one germination point is the one that had the plate sprayed. Both looking very clean so far!


3-4-24
All germination plates today are now showing heavy trich contamination. The two plates treated twice with h2o2 lagged behind. There’s other germination points beginning to develop so I took a transfer (in an SAB) to a new plate. Although now it’s clearly evident, that even with the help of h2o2, you’ll still transfer difficult trich from fruit to swab and then to plate. Now I’m curious if h2o2 will give you a step ahead in battling the trich to receive a clean end result…. Trich is such a bitch!


3-6-24
The transfer I took from 3-4 is showing good signs of growth so far. (Sorry for the dyed agar, I’m seeing it’s hard to see clearly in picture.)

Recall the monos I pulled from 3-3, before the trich showed itself? That plate is doing well today too! 3 transfers on one plate below.

As of now, I believe treating trich infested fruits before swabbing, and then treating the swabs with h202 before taking them to agar doesn’t eliminate trich. However, it’s apparent that it cripples it long enough for you to be able to develop clean germination and then transfer.

Best course of action, per observation, for needing spores from a trich infested grow. Harvest fruit, spray thoroughly with h2o2, swab fruit, let dry, spray swab with h2o2, germinate plate, transfer sections upon first signs of growth.
Experiment concluded!
Next Experiment
2-26-24
I have some bacterial plates here, I pulled the donor wedges back from the plates and placed one on a BRF puck (can’t lose this culture) and one on a new plate. the bacterial dishes and lids were then sprayed just one time with H2O2 and then resealed. After pulling the wedges, there were still minute traces of mycelium on the dish.

2-28-24
The minute traces of mycelium are flourishing today and the bacteria seems to have taken a hit. I sprayed each plate again today with just one spray of h2o2


My transfer from 2-26 of these plates to a new plate looks like this today.

I didn’t spray the plates before transferring and I wish I would have. So now I’ve pulled this wedge again, sprayed it, and then transferred to a new plate again and disposed of the donor plate.

3-1-24
The new transfer isnt doing well. Bacteria has hung on with the transfer.

I’m not going to transfer this again. I’m calling it a loss and am going to keep “treating” the original plates periodically with h2o2 as they seem to be doing well!




3-3-24
The bacterial leucistic red boy plates continue to be treated almost daily. They seem to be doing ok on top but from underneath, you can see the bacteria growing with the mycelium.





This test almost seems ridiculous to do considering a brf puck would save it immediately. I’m still curious though 🤷♂️
3-6-24
It appears that treating the plates daily with h2o2 stops the bacteria from outgrowing the mycelium. However, the bacteria remains alive and strong underneath the mycelium, as you can see in the pictures below.




I decided it wasn’t worth it to keep treating but instead I’ll spray once more and take two transfers from one plate furthest away from the bacteria as possible. One transfer will be “agar sandwiched” (I’ve always wanted to try this haha) to prevent potential bacteria.




3-13-24
Both transfers are officially doing well!


However, the piece I transferred to a brf puck and then back to agar is doing even better!

Best course of action: transfer any bacterial wedges to BRF puck or agar sandwich (more experimentation needed.) h2o2 certainly seems to stall bacteria longer than it does the healthy mycelium. However, it’s unnecessary for this method considering BRF will defeat it easier.
Experiment concluded!
Next Experiment
2-27-24
Subtropicalis fruit picked and sliced into 3 pieces with exteriors of each piece intact. A cap to one nutrient petri, a stipe piece to each; an additional nutrient plate and a brf. (I suspect possibly some bacteria will still come)





This experiment is not going well. The cap has some decent size bacteria forming (this plate has reached the end of the experiment.)

The stipe section I took to agar has bacteria is several spots as well, but I’m going to begin “treating” it periodically with h2o2 just like with the Leucistic Redboy experiment. I will see if it can be saved without early transfers.


The brf stipe clone seems to be doing well. I won’t transfer from this until mycelium growth has reached far enough away from the fruit.

3-3-24
I’ve decided to run this test again but instead, I soaked the fruit in h2o2 and flipped it after 5 minutes. 10 minute total soak and there was still minor foaming after 10 minutes, which I believe would indicate the peroxide was still active. I transferred two stipe sections. Considering how bacterial the cap was on the last test, it wasn’t even worth attempting.



Back to the original stipe section plate that I decided to treat, labeled “C1b” is doing far from well. I would like to toss this plate and call it loss. However, I’m going to keep treating it (hopefully daily (didn’t yesterday)) with h2o2 and see what happens.

I could actually smell the bacteria today when I removed the lid to quickly snap a good picture and spray it!
3-6-24
Plate “c1b” was tossed.. GROSS! The bacteria had so much of a head start on the mycelium to the point where I don’t even believe brf could save it!
The new fruit h2o2 soaked clones appear to be doing well today. (Forgot to take pictures today 🤦♂️)
3-13-24
Bacteria forming on both plates of the soaked cloned. I haven’t checked these since my last update and have been slacking hard 🤦♂️


I believe this could be settled in one transfer alone from the better plate, and I believe most would agree. However I don’t care to chase it any longer as it’s not even clone I truly wished to keep outside of this experiment.
I believe the best course of action again for potential bacteria, would be to transfer to brf puck. Secondary to that, would be fruit h2o2 soak followed by nutrient agar transfers.
3-18
I was going to toss these plates but forgot to. It seems the bacteria has actually stop growing 🙀 look at the mycelium just out running it!

Experiment concluded!
Next Experiment
2-23-24
Went back to an old spore print of natalensis from when I first started growing again, back in October, after a 10 year break. I knew for sure the print would be dirty because it was amateur hour for me. I sprayed one swab with h2o2 and didn’t spray the other, then pulled spores and streaked plates. The results:




Yellow plate is without h2o2 and it has bacteria forming, circled in purple. The red plate was used with the h2o2 swab. It has no visible bacteria yet. Both plates have monokaryons as well! I’ll pull them from the red plate of course! Afterwards, I’ll leave these plates to grow to see if any bacteria pops up on the red.
3-1-24
The untreated swab plate still has the same bacteria (although it hasn’t grown much) and the treated swab plates is doing perfectly fine still even after pulling monokaryons.


3-14-24
Pictured below is the original germination plate from the swab sprayed with h2o2. Looks beautiful! Could even be sent straight to grain but I still would never recommend taking a germ plate to grain.

Experiment concluded!
Next Experiment
2-28-24
Okay, this one I’m pretty excited to test out. I have a clearly unhealthy jar of grain spawn here. Throwing it into a grow bag, spraying it with h2o2 and mixing it all about and immediately adding substrate.
Note: while spraying the grains with h2o2 I noticed immediate foaming and you could hear it too. You may have noticed this before when cleaning a fresh wound. There’s a possibility that this could be a method in determining if questionable grains are bacterial (although these ones clearly were upon visual inspection alone.) Further testing may be required.




3-26-24
Fail! I’m going to try soaking the grains in h2o2 on the next go around!


Results pending…
Edited by SupaThaRipper (04/02/24 11:02 AM)
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3.A.M
Boop!



Registered: 10/17/22
Posts: 1,407
Loc: Oz
Last seen: 1 hour, 2 minutes
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper] 2
#28678291 - 02/27/24 02:05 PM (3 months, 27 days ago) |
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😂
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
Loc: USA
Last seen: 5 hours, 52 minutes
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] 3
#28678296 - 02/27/24 02:10 PM (3 months, 27 days ago) |
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lol this isn’t a “let me get under peoples skin” post. It’s a “this needs to be explored again” post haha
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3.A.M
Boop!



Registered: 10/17/22
Posts: 1,407
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper]
#28678302 - 02/27/24 02:14 PM (3 months, 27 days ago) |
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I know that, I’m still gonna take the piss though (: I’ll be serious from here on, I swear 😐
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
Loc: USA
Last seen: 5 hours, 52 minutes
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] 1
#28678313 - 02/27/24 02:24 PM (3 months, 27 days ago) |
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🤣 no seriousness required 🤣
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Bra
above and beyond

Registered: 07/12/19
Posts: 511
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper]
#28678378 - 02/27/24 03:12 PM (3 months, 27 days ago) |
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I don't recommend it personally, but when I was using H2O2 to soak grains for about 3 hours with addition of H2O2 after pure water soak, it was foaming and then jars were colonized faster and I had less contams with MSS. I've tried this several times and observed that any time. Maybe I had to do more attempts to have more cases to compare. My 2 cents, I know, clear culture on agar is the key, I just recalled my trials, and I'm excited to see your results, Supa.
-------------------- Bra's spores giveaway Journey of a Letter PM me, if you're a cannabis grower and you'd like to get my own-breed photoperiodic feminized SEEDS for free.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
Loc: USA
Last seen: 5 hours, 52 minutes
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Bra]
#28678400 - 02/27/24 03:27 PM (3 months, 27 days ago) |
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Wow that’s crazy! See this is what I love to hear!
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alaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,962
Loc: The 49th Dimension
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper] 1
#28678428 - 02/27/24 03:44 PM (3 months, 27 days ago) |
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It has its uses. If a person flips through enough posts from the vaults on here they'll come across TCs talking about using it for some very specific task (then treating it like leprosy elsewhere...). Don't get me wrong: I understand that recommending a specific use for it turns into a thing, but... still. Frankly - despite being depicted as possibly destructive on a cellular level and thus impeding recovery - I have found that using it in my jars of sterilized water for serial dilution for wild clones leads to better outcomes. It's a godsend for shit like wild bear tooth or ganoderma which have football bat success rates.
-------------------- THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES "It is all one vast awakened thing. I call it the golden eternity. It is perfect." -Kerouac
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,253
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I’ve noticed it’ll slow mycelium growth, so it definitely does hurt it but only for a little bit and mycelium recovers quick! meanwhile, whatever else you were attacking is dead! I’m hoping to give definitive answers and put it back in the light!
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,007
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper]
#28678764 - 02/27/24 06:40 PM (3 months, 27 days ago) |
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I used h2o2 when I started out during the pandemic and couldn't get iso. Was a noob, well a bigger noob, then but did alright. I think it has a place for more experienced users or in a pinch, but there are probably more fool proof methods. I am interested to see how the surface clone works out. Could be useful with brf puck cloning those pesky tiny stipe fruits.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: ReverendMyc]
#28678775 - 02/27/24 06:45 PM (3 months, 27 days ago) |
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I’m glad you said that! I have some subtropicalis fruits ready to get yanked right now. I’ll throw an entire fruit body on a plate and see what happens!
I’ll update the original post above in a few minutes!
Edit: I’ll have some pans to run in a couple weeks! Subtropicalis is still way too big lol
Edited by SupaThaRipper (02/27/24 07:03 PM)
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3.A.M
Boop!



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Bra] 1
#28678982 - 02/27/24 09:36 PM (3 months, 27 days ago) |
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Quote:
Bra said: I don't recommend it personally, but when I was using H2O2 to soak grains for about 3 hours with addition of H2O2 after pure water soak, it was foaming and then jars were colonized faster and I had less contams with MSS. I've tried this several times and observed that any time. Maybe I had to do more attempts to have more cases to compare. My 2 cents, I know, clear culture on agar is the key, I just recalled my trials, and I'm excited to see your results, Supa.
Ok, this. Anyone else trialed it for cleaning dirty grain? I know Josex has a thread dedicated to dealing with shit grain but I found even with lime soaking certain grains it was a bust, got about 6 bags of grain sitting here, circled in salt and surrounded by crucifixes I might be able to finally do something with. What type of grains were you working with?
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SupaThaRipper
Genetics Hoarder



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] 1
#28679343 - 02/28/24 09:02 AM (3 months, 26 days ago) |
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🤣 try holy water?
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Mwj12977
OLD DAD


Registered: 12/12/23
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper] 1
#28679359 - 02/28/24 09:17 AM (3 months, 26 days ago) |
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I use it for cleaning stuff. But I make my own from 35% food grade peroxide. I’ll take it down to around 6%. I try to keep it at a threshold that it won’t turn my skin white. Food grade is different than what you get from the store. It doesn’t have the additives
Years ago when I started out I used to spray it on contamination spots. I don’t know how well it worked but I got lots of fruit and not much contamination. I would spray it on cakes in between flushes after harvest/dunk before I added a new top layer.
Fast forward to 2024 I use it to clean my tubs and I use it when I’m inoculating with syringes. I actually don’t flame anything. I use a 10% solution and simply dip the needle in between pokes.
My current projects are very successful with no contaminates and I’m not using it nearly as much as I used too. Or in the same ways I’m used too.
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SupaThaRipper
Genetics Hoarder



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Mwj12977] 1
#28679387 - 02/28/24 09:45 AM (3 months, 26 days ago) |
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Thank you for the input man. I love to hear it! The stuff I’m using is only 3% from the stores. I’ll keep running with that for now because it’ll be more easily obtainable for everyone if these experiments are successful
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BeefSupremeJr
Detritivore



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper] 2
#28679419 - 02/28/24 10:11 AM (3 months, 26 days ago) |
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Ok this is hilarious and made my whole fucking day. I dont even care about the efficacy of h202 anymore, Im just flabbergasted at how good of a dude you apparently are. Amazing. This is awesome.
Okay but what about adding a drop of dish soap to break the surface tension of it? Thats really been my biggest complaint of h202 from the get-go. It beads rather than coats.
I appreciate this work so much. As ive said, i want h202 to work. Largely because it doesnt smell like shit but also because its functionality is just brutal and interesting.
I am pitching a tent. I am here for this. Beef is with it. Carry on my brother in cult.
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SupaThaRipper
Genetics Hoarder



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefSupremeJr] 1
#28679423 - 02/28/24 10:15 AM (3 months, 26 days ago) |
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Haha at first I thought you were going to start hurting my feelings lol but I’m ecstatic to see you’re on board! I suppose I could add a drop of polysorbate(thanks Stipe,) as it does bead!
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BeefSupremeJr
Detritivore



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: SupaThaRipper] 1
#28679428 - 02/28/24 10:18 AM (3 months, 26 days ago) |
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Im sorry if i was ever harsh man. please forgive me. We just have different agendas i see that now. check your ratings
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Mwj12977
OLD DAD


Registered: 12/12/23
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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefSupremeJr]
#28679479 - 02/28/24 11:00 AM (3 months, 26 days ago) |
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One of the main reasons I use it is because it doesn’t smell like shit!
I think figuring out when, where and how to use it would be beneficial for people. Cautionary as opposed to reactionary. If someone thinks they are going to fix a trich issue with it, they won’t. But it can definitely help prevent a trich issue. Better than a k95 can prevent covid 😂.
Anyway both of you guys are great and at the end of the day we all hate UB TEK! That’s what really matters!
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tree frog
eats bugs



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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Mwj12977]
#28679660 - 02/28/24 01:01 PM (3 months, 26 days ago) |
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Following with interest. I've been trying to get some wild clones of a choice edible the last two seasons and so far it's been lots of failure.
Had never considered spraying the bacteria directly! Cool stuff brother.
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