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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Scales, Wild Bird Seed

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OfflineCyber
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Experiment using Pectin in place of agar
    #2867923 - 07/08/04 08:44 AM (13 years, 10 months ago)

Well since no one seems to have tried it, and Anno asked me to try it and report back I am starting a new thread. I will outline the experiment first. I am looking for any comments or suggestions. People are welcome to start a betting pool as well :wink:

1) determan the amount of pectin needed to get the proper jelling effect.
2) Use it to make PDA (I guess it would be PDP).
3) Put in 1/2 pint jars and PC.
4) Inoculate and monitor growth.


Edited by Cyber (07/08/04 03:05 PM)


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OfflineCyber
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Re: Experiment using Pectin in place of agar [Re: Cyber]
    #2868872 - 07/08/04 03:13 PM (13 years, 10 months ago)

Ok first update!

I picked up pectin at the store.



You will notice that Pectin contains dextrose!

I did several trials and settled on the following mix

150g of potatoes boiled for 30 min in 500ml of water.

take 250ml of the water
add to it 247.78 grains of pectin (19 grams, sorry my scale measures in grains)

PC for 20 min @ 15psi.

I then placed 1/4 of an inch in the bottom of several 1/2 pint jars and am letting it cool. Pectin takes time to set up (Gel) So I should know by tomorrow. Although it is becoming a viscus fluid as I type this.


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Invisiblewhiterasta
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Re: Experiment using Pectin in place of agar [Re: Cyber]
    #2869558 - 07/08/04 07:06 PM (13 years, 10 months ago)

I would make a WBS flour and blend into your pectin to give it body.Pectin liquifies much easier than agar so I have found it only an able substitute when a thickening agent such as flour or sawdust(or a blend) is incorporated. Pectin lends itself well to peroxidation and the use of antibiotics in vitro and seem slightly more prone to contamination than agar.
Good luck
WR:wexican:


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Offlinepoorphucker
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Re: Experiment using Pectin in place of agar *DELETED* [Re: Cyber]
    #2870157 - 07/08/04 09:55 PM (13 years, 10 months ago)

Post deleted by poorphucker


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And YOU CANNOT FLY...unless you are on a 20 story building and jump! Practice makes perfect!


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Offlinedebianlinux
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Re: Experiment using Pectin in place of agar [Re: poorphucker]
    #2870241 - 07/08/04 10:13 PM (13 years, 10 months ago)

too much acid man, too much!

seriously though, starting with an acidic "substrate" is a bad idea.


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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Scales, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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