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OfflinetheAMAZINGrabbit
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Registered: 01/09/24
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Is My Grain Ready for Birthing and Agar/Grain Transfer
    #28670909 - 02/22/24 06:20 AM (10 months, 19 days ago)

Hello! Does my grain spawn look ready at this point?


I inoculated 6 jars with spores and of them only this one seemed to take off.
I plan on transferring some of the grain to agar and some to more grain and then birthing the rest in a small shoebox. Does this sound solid?

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OfflineRockinRobot
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit] * 1
    #28670911 - 02/22/24 06:22 AM (10 months, 19 days ago)

Sorry but that doesn't look good. Spores to grain is bad.

Read the Hitchhikers guide.

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OfflineAspectOfTheCreator
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit]
    #28670916 - 02/22/24 06:28 AM (10 months, 19 days ago)

:whathesaid:

Some may tell you to top fruit it but personally id start over.


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Offlinefiddle_head
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit]
    #28670917 - 02/22/24 06:31 AM (10 months, 19 days ago)

those jars are bacterial as hell, youd be so lucky to get fruit from that. indeed you must start over. do not inject spores to anything but a pf cake. get clean culture on agar or use lc to inject. (liquid culture), you must also test that on agar to make sure its not tainted.


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OfflinetheAMAZINGrabbit
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit]
    #28670927 - 02/22/24 06:41 AM (10 months, 19 days ago)

OK Noted, I have one more syringe of spores I will send straight to agar hopefully I get it right.

Edited by theAMAZINGrabbit (02/22/24 06:42 AM)

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Offlinefiddle_head
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit]
    #28670932 - 02/22/24 06:50 AM (10 months, 19 days ago)

yes germinate your spores on agar. you have sab or flow hood yes?

after you germinate you will want to take small transfers and donate to a blank plate until you have clean culture.


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OfflinetheAMAZINGrabbit
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: fiddle_head]
    #28670943 - 02/22/24 07:08 AM (10 months, 19 days ago)

Yes I have a still air box I will pour more agar and hopefully get some good growth.

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OfflinetheAMAZINGrabbit
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: fiddle_head]
    #28678553 - 02/27/24 04:40 PM (10 months, 14 days ago)

What makes you guys think that? I just opened it and gave it a whiff and it didn't smell sweet or sour or anything. My sense of smell is a little weak but I didn't get anything strong. Does it help knowing I am using brown rice as my grain?

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OfflineAspectOfTheCreator
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Re: Is My Grain Ready for Birthing and Agar/Grain Transfer [Re: theAMAZINGrabbit]
    #28678743 - 02/27/24 06:31 PM (10 months, 14 days ago)

The smell test is worthless. You get an eye for this stuff once youve done it for awhile. Those jars are highly contaminated.


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