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Supa’s LIVE/UNCUT H2O2 experiments! * 10
    #28677833 -

Auto-Summary
The author shares multiple experiments involving the use of hydrogen peroxide (H2O2) on contaminated fruits and growing mediums. The experiments aim to study preventative measures against contamination, particularly trichomes. Results show that while H2O2 may help in reducing contamination, it does not completely eliminate it. The best course of action appears to be harvesting fruits, spraying them with H2O2, swabbing, and then treating the swabs with H2O2 before culturing. Additionally, treating bacterial plates and germination areas with H2O2 can help manage bacterial growth. The author concludes that H2O2 can be a useful tool in managing contamination, but it is not a guaranteed solution and should be used in conjunction with proper hygiene and sterile techniques.

I’ve been experimenting with the potential benefits of H2O2. This subject is highly debated and most scientific studies would push you away from H2O2. Currently I have no intentions of ever trying to save a grow with H2O2. This thread will be more so for studying preventative measures. I’ll experiment and post live and uncut results to give everyone as clear of results as possible. ALL FAILURES WILL BE POSTED as I go along.




Don’t expect this thread to be organized or anything like that as I’ll have multiple experiments going at once. All hate comments welcomed 🤣 Now that being said, experimenting with H2O2 is not something currently recommended to new growers (I’m hoping to eventually change that) In the meantime, follow a tried and trusted tek to the T and if you come across contaminations and are unclear about how to proceed, try asking in the quick questions thread or use the search function to see if its already been answered recently. 🍻

DO NOT DO WHAT I DO IN THIS THREAD!! THESE ARE UNSANITARY PRACTICES AND DOING SO WILL SURELY LEAVE YOU WITH NOTHING BUT FAILURES!

Let’s get into it.

2-26-24

While harvesting a fruit I intended to clone, I dropped it on the floor, went to pick it up with my toes and accidentally kicked it across the room 🤦‍♂️ clumsy me!



Finally, I managed to snatch the little bastard!



I sprayed the fruit generously with H2O2 and placed it in front of my ffu to let it dry. Then took a tissue sample to clone from the exterior of the fruit.



I also cloned from the interior, to ensure I don’t lose this clone all together.

2-28-24

The exterior clone tissue plate has zero signs so far of bacteria or any other contamination. The mycelium has started to show itself.




3-1-24

The exterior clone plate is doing well. No signs of contamination still as the mycelium continues to grow. This will be the last update for a while until significant growth has occurred or until a contamination appears. ( don’t want to bog this post down with too many pictures)



3-3-24 (I lied lol)

Here’s another update of the exterior clone plate again today



3-13-24




Experiment concluded!

Next Experiment

1-21-24

I have a grow bag loaded with the notorious trich! I picked various fruits with some directly above and gradually leading away, with one as far as way from the trich as possible. All fruits were generously sprayed with H2O2 and left to dry in front of the ffu.





I tried finding these swabs briefly yesterday to take to agar but couldn’t 🤦‍♂️ no worries though, I will today!

2-28-24

I didn’t find the swabs yesterday but I found them today. I took the 2 most hazardous swabs from fruits in direct contact with the trich. The plates labeled “h2o2x2” means they were sprayed again once today during inoculation of the agar. One plate had the swab sprayed before streaking. One had the plate itself sprayed after streaking. The plates labeled “h2o2x1” means they only had the initial spray of the fruits during harvesting and swabbing. No additional sprays were done.



3-1-24

No signs of growth from any of the four plates (contaminants or mycelium.) will hold of on picture for now.

3-3-24

Both plates with only the initial fruits sprayed are clearly contaminated with what I’m sure will be trich.





However, the plates that were treated twice with h2o2 are doing well today! Tops removed for clearer pictures. The plate with several germination had the swab sprayed. I pulled potential monos from this plate. The plate with only one germination point is the one that had the plate sprayed. Both looking very clean so far!




3-4-24

All germination plates today are now showing heavy trich contamination. The two plates treated twice with h2o2 lagged behind. There’s other germination points beginning to develop so I took a transfer (in an SAB) to a new plate. Although now it’s clearly evident, that even with the help of h2o2, you’ll still transfer difficult trich from fruit to swab and then to plate. Now I’m curious if h2o2 will give you a step ahead in battling the trich to receive a clean end result…. Trich is such a bitch!




3-6-24

The transfer I took from 3-4 is showing good signs of growth so far. (Sorry for the dyed agar, I’m seeing it’s hard to see clearly in picture.)



Recall the monos I pulled from 3-3, before the trich showed itself? That plate is doing well today too! 3 transfers on one plate below.



As of now, I believe treating trich infested fruits before swabbing, and then treating the swabs with h202 before taking them to agar doesn’t eliminate trich. However, it’s apparent that it cripples it long enough for you to be able to develop clean germination and then transfer.



Best course of action, per observation, for needing spores from a trich infested grow. Harvest fruit, spray thoroughly with h2o2, swab fruit, let dry, spray swab with h2o2, germinate plate, transfer sections upon first signs of growth.

Experiment concluded!

Next Experiment

2-26-24

I have some bacterial plates here, I pulled the donor wedges back from the plates and placed one on a BRF puck (can’t lose this culture) and one on a new plate. the bacterial dishes and lids were then sprayed just one time with H2O2 and then resealed. After pulling the wedges, there were still minute traces of mycelium on the dish.



2-28-24

The minute traces of mycelium are flourishing today and the bacteria seems to have taken a hit. I sprayed each plate again today with just one spray of h2o2




My transfer from 2-26 of these plates to a new plate looks like this today.



I didn’t spray the plates before transferring and I wish I would have. So now I’ve pulled this wedge again, sprayed it, and then transferred to a new plate again and disposed of the donor plate.



3-1-24

The new transfer isnt doing well. Bacteria has hung on with the transfer.



I’m not going to transfer this again. I’m calling it a loss and am going to keep “treating” the original plates periodically with h2o2 as they seem to be doing well!






3-3-24

The bacterial leucistic red boy plates continue to be treated almost daily. They seem to be doing ok on top but from underneath, you can see the bacteria growing with the mycelium.







This test almost seems ridiculous to do considering a brf puck would save it immediately. I’m still curious though 🤷‍♂️

3-6-24

It appears that treating the plates daily with h2o2 stops the bacteria from outgrowing the mycelium. However, the bacteria remains alive and strong underneath the mycelium, as you can see in the pictures below.






I decided it wasn’t worth it to keep treating but instead I’ll spray once more and take two transfers from one plate furthest away from the bacteria as possible. One transfer will be “agar sandwiched” (I’ve always wanted to try this haha) to prevent potential bacteria.






3-13-24

Both transfers are officially doing well!




However, the piece I transferred to a brf puck and then back to agar is doing even better!



Best course of action: transfer any bacterial wedges to BRF puck or agar sandwich (more experimentation needed.) h2o2 certainly seems to stall bacteria longer than it does the healthy mycelium. However, it’s unnecessary for this method considering BRF will defeat it easier.

Experiment concluded!

Next Experiment

2-27-24

Subtropicalis fruit picked and sliced into 3 pieces with exteriors of each piece intact. A cap to one nutrient petri, a stipe piece to each; an additional nutrient plate and a brf. (I suspect possibly some bacteria will still come)







This experiment is not going well. The cap has some decent size bacteria forming (this plate has reached the end of the experiment.)



The stipe section I took to agar has bacteria is several spots as well, but I’m going to begin “treating” it periodically with h2o2 just like with the Leucistic Redboy experiment. I will see if it can be saved without early transfers.




The brf stipe clone seems to be doing well. I won’t transfer from this until mycelium growth has reached far enough away from the fruit.



3-3-24

I’ve decided to run this test again but instead, I soaked the fruit in h2o2 and flipped it after 5 minutes. 10 minute total soak and there was still minor foaming after 10 minutes, which I believe would indicate the peroxide was still active. I transferred two stipe sections. Considering how bacterial the cap was on the last test, it wasn’t even worth attempting.





Back to the original stipe section plate that I decided to treat, labeled “C1b” is doing far from well. I would like to toss this plate and call it loss. However, I’m going to keep treating it (hopefully daily (didn’t yesterday)) with h2o2 and see what happens.



I could actually smell the bacteria today when I removed the lid to quickly snap a good picture and spray it!

3-6-24

Plate “c1b” was tossed.. GROSS! The bacteria had so much of a head start on the mycelium to the point where I don’t even believe brf could save it!

The new fruit h2o2 soaked clones appear to be doing well today. (Forgot to take pictures today 🤦‍♂️)

3-13-24

Bacteria forming on both plates of the soaked cloned. I haven’t checked these since my last update and have been slacking hard 🤦‍♂️




I believe this could be settled in one transfer alone from the better plate, and I believe most would agree. However I don’t care to chase it any longer as it’s not even clone I truly wished to keep outside of this experiment.

I believe the best course of action again for potential bacteria, would be to transfer to brf puck. Secondary to that, would be fruit h2o2 soak followed by nutrient agar transfers.

3-18

I was going to toss these plates but forgot to. It seems the bacteria has actually stop growing 🙀 look at the mycelium just out running it!



Experiment concluded!

Next Experiment

2-23-24

Went back to an old spore print of natalensis from when I first started growing again, back in October, after a 10 year break. I knew for sure the print would be dirty because it was amateur hour for me. I sprayed one swab with h2o2 and didn’t spray the other, then pulled spores and streaked plates. The results:






Yellow plate is without h2o2 and it has bacteria forming, circled in purple. The red plate was used with the h2o2 swab. It has no visible bacteria yet. Both plates have monokaryons as well! I’ll pull them from the red plate of course! Afterwards, I’ll leave these plates to grow to see if any bacteria pops up on the red.

3-1-24

The untreated swab plate still has the same bacteria (although it hasn’t grown much) and the treated swab plates is doing perfectly fine still even after pulling monokaryons.




3-14-24

Pictured below is the original germination plate from the swab sprayed with h2o2. Looks beautiful! Could even be sent straight to grain but I still would never recommend taking a germ plate to grain.



Experiment concluded!

Next Experiment

2-28-24

Okay, this one I’m pretty excited to test out. I have a clearly unhealthy jar of grain spawn here. Throwing it into a grow bag, spraying it with h2o2 and mixing it all about and immediately adding substrate.

Note: while spraying the grains with h2o2 I noticed immediate foaming and you could hear it too. You may have noticed this before when cleaning a fresh wound. There’s a possibility that this could be a method in determining if questionable grains are bacterial (although these ones clearly were upon visual inspection alone.) Further testing may be required.






3-26-24

Fail! I’m going to try soaking the grains in h2o2 on the next go around!




Results pending…

Edited by PhenoDreamers (04/02/24 11:02 AM)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 2
    #28678291 -

😂


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] * 3
    #28678296 -

lol this isn’t a “let me get under peoples skin” post. It’s a “this needs to be explored again” post haha

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28678302 -

I know that, I’m still gonna take the piss though (:
I’ll be serious from here on, I swear 😐


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] * 1
    #28678313 -

🤣 no seriousness required 🤣

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28678378 -

I don't recommend it personally, but when I was using H2O2 to soak grains for about 3 hours with addition of H2O2 after pure water soak, it was foaming and then jars were colonized faster and I had less contams with MSS. I've tried this several times and observed that any time. Maybe I had to do more attempts to have more cases to compare.
My 2 cents, I know, clear culture on agar is the key, I just recalled my trials, and I'm excited to see your results, Supa.


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Bra]
    #28678400 -

Wow that’s crazy! See this is what I love to hear!

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28678428 -

It has its uses.  If a person flips through enough posts from the vaults on here they'll come across TCs talking about using it for some very specific task (then treating it like leprosy elsewhere...). Don't get me wrong: I understand that recommending a specific use for it turns into a thing, but... still. Frankly - despite being depicted as possibly destructive on a cellular level and thus impeding recovery - I have found that using it in my jars of sterilized water for serial dilution for wild clones leads to better outcomes.  It's a godsend for shit like wild bear tooth or ganoderma which have football bat success rates.


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THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: alaskappalachian] * 2
    #28678456 -

I’ve noticed it’ll slow mycelium growth, so it definitely does hurt it but only for a little bit and mycelium recovers quick! meanwhile, whatever else you were attacking is dead! I’m hoping to give definitive answers and put it back in the light!

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28678764 -

I used h2o2 when I started out during the pandemic and couldn't get iso. Was a noob, well a bigger noob, then but did alright. I think it has a place for more experienced users or in a pinch, but there are probably more fool proof methods. I am interested to see how the surface clone works out. Could be useful with brf puck cloning those pesky tiny stipe fruits.
:popcorn:


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: ReverendMyc]
    #28678775 -

I’m glad you said that! I have some subtropicalis fruits ready to get yanked right now. I’ll throw an entire fruit body on a plate and see what happens!

I’ll update the original post above in a few minutes!

Edit: I’ll have some pans to run in a couple weeks! Subtropicalis is still way too big lol

Edited by PhenoDreamers (02/27/24 07:03 PM)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Bra] * 1
    #28678982 -

Quote:

Bra said:
I don't recommend it personally, but when I was using H2O2 to soak grains for about 3 hours with addition of H2O2 after pure water soak, it was foaming and then jars were colonized faster and I had less contams with MSS. I've tried this several times and observed that any time. Maybe I had to do more attempts to have more cases to compare.
My 2 cents, I know, clear culture on agar is the key, I just recalled my trials, and I'm excited to see your results, Supa.


Ok, this.
Anyone else trialed it for cleaning dirty grain?
I know Josex has a thread dedicated to dealing with shit grain but I found even with lime soaking certain grains it was a bust, got about 6 bags of grain sitting here, circled in salt and surrounded by crucifixes I might be able to finally do something with.
What type of grains were you working with?


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] * 1
    #28679343 -

🤣 try holy water?

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28679359 -

I use it for cleaning stuff. But I make my own from 35% food grade peroxide. I’ll take it down to around 6%. I try to keep it at a threshold that it won’t turn my skin white. Food grade is different than what you get from the store. It doesn’t have the additives

Years ago when I started out I used to spray it on contamination spots. I don’t know how well it worked but I got lots of fruit and not much contamination.  I would spray it on cakes in between flushes after harvest/dunk before I added a new top layer.

Fast forward to 2024 I use it to clean my tubs and I use it when I’m inoculating with syringes. I actually don’t flame anything. I use a 10% solution and simply dip the needle in between pokes.

My current projects are very successful with no contaminates and I’m not using it nearly as much as I used too. Or in the same ways I’m used too.


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The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77] * 1
    #28679387 -

Thank you for the input man. I love to hear it! The stuff I’m using is only 3% from the stores. I’ll keep running with that for now because it’ll be more easily obtainable for everyone if these experiments are successful

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 2
    #28679419 -

Ok this is hilarious and made my whole fucking day.  I dont even care about the efficacy of h202 anymore, Im
just flabbergasted at how good of a dude you apparently are.  Amazing.  This is awesome. 

Okay but what about adding a drop of dish soap to break the surface tension of it?  Thats really been my biggest complaint of h202 from the get-go.  It beads rather than coats.

I appreciate this work so much.  As ive said, i want h202 to work.  Largely because it doesnt smell like shit but also because its functionality is just brutal and interesting. 

I am pitching a tent.  I am here for this.  Beef is with it.  Carry on my brother in cult. 

:yeahthatsright:


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The Hitchhiker's Guide To The Shroomery
My Cultivation Youtube Channel

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefExtreme] * 1
    #28679423 -

Haha at first I thought you were going to start hurting my feelings lol but I’m ecstatic to see you’re on board! I suppose I could add a drop of polysorbate(thanks Stipe,) as it does bead!

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28679428 -

Im sorry if i was ever harsh man.  please forgive me.  We just have different agendas i see that now. check your ratings


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The Hitchhiker's Guide To The Shroomery
My Cultivation Youtube Channel

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefExtreme]
    #28679479 -

One of the main reasons I use it is because it doesn’t smell like shit!

I think figuring out when, where and how to use it would be beneficial for people. Cautionary as opposed to reactionary. If someone thinks they are going to fix a trich issue with it, they won’t. But it can definitely help prevent a trich issue. Better than a k95 can prevent covid 😂.

Anyway both of you guys are great and at the end of the day we all hate UB TEK! That’s what really matters!


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28679660 -

Following with interest.  I've been trying to get some wild clones of a choice edible the last two seasons and so far it's been lots of failure.

Had never considered spraying the bacteria directly!  Cool stuff brother.


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: tree frog] * 1
    #28679673 -

:threadmonitor:

Supa, you are using H2O2 on fruits before cloning to reduce contamination. Have you tried doing a side-by-side with isopropyl alcohol? Take a couple fruits, toss them both on the floor and get 'em dirty. Spray one with H2O2 and one with isopropyl, and see how the results compare.


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Important Links.

Be kind to others.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: joze]
    #28679698 -

I have not. I havent pursued iso studying as much as I have with hydrogen peroxide. I honestly don’t have much of a leg to stand on right now when even talking about iso haha. Doesn’t I cause more damage to the mycelium? Also, I’ve read somewhere that hydrogen peroxide fights mold better. Now don’t quote me on this because I can’t even find the source of that anymore. It’s worth experimenting though, everything is and that’s for sure!

The problem with trying to find solid information on hydrogen peroxide is that any study you find will not correlate directly to cultivating. If you type in such, it’ll only give you shroomery results, and the notorious, REDDIT! 😱 And if you try to find what kills mold without bringing up mycelium, it’ll tell you bleach 😂 and we all know that bleach kills everything. There’s not simple way of searching for what kills contamination but not mycelium 😂

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: tree frog]
    #28679703 -

Quote:

tree frog said:
Following with interest.  I've been trying to get some wild clones of a choice edible the last two seasons and so far it's been lots of failure.

Had never considered spraying the bacteria directly!  Cool stuff brother.




Feel free to post your results here 😉 😂

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28679714 -

:popcorn:

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 2
    #28679754 -

Quote:

SupaThaRipper said:
I have not. I havent pursued iso studying as much as I have with hydrogen peroxide. I honestly don’t have much of a leg to stand on right now when even talking about iso haha. Doesn’t I cause more damage to the mycelium? Also, I’ve read somewhere that hydrogen peroxide fights mold better. Now don’t quote me on this because I can’t even find the source of that anymore. It’s worth experimenting though, everything is and that’s for sure!

The problem with trying to find solid information on hydrogen peroxide is that any study you find will not correlate directly to cultivating. If you type in such, it’ll only give you shroomery results, and the notorious, REDDIT! 😱 And if you try to find what kills mold without bringing up mycelium, it’ll tell you bleach 😂 and we all know that bleach kills everything. There’s not simple way of searching for what kills contamination but not mycelium 😂


Here's a starting point for you...

Novel Perspective of Medicinal Mushroom Cultivations: A Review Case for ‘Magic’ Mushrooms. Sommano et al., 2022.

The authors barely touch on it, but in the conclusion they seem to imply that Psilocybe produce peroxidase. This would imply some ability to deal with peroxides.

"The magic mushrooms of Psilocybe cubensis and P. baeocystis were submerged and grown in the media and it was discovered that the mycelium and sclerotia were excellent sources of psilocybin and psilocin. Another industrial application of medicinal mushrooms is that they also contain enzyme that preferentially degrade lignin, exposing cellulose as feed for animals such as ruminants. It also bioproduces laccase, magnesium peroxidase, and versatile peroxidase for the pulp and paper industry."


Efficacy of Hydrogen Peroxide on Postharvest Quality of White Button Mushroom. Sharaf-Eldin & Geösel, 2016.

"Mushrooms were immersed after harvesting in 1, 2 or 4% of hydrogen peroxide comparing to immerse in water (control). The results showed that hydrogen peroxide treatments were effective for keeping eating quality, nutritional value, sensory attributes and microbial safety during storage due to decrease the degradation in colour, composition and hardness as well as reducing weight loss and microbial load."


Hydrogen peroxide concentration measured in cultivation substrates during growth and fruiting of the mushrooms Agaricus bisporus and Pleurotus spp. Savoie et al., 2007.

"Hydrogen peroxide (H2O2) has been implicated in lignin and cellulose-degrading systems of both white-rot and brown-rot basidiomycete fungi...

This molecule could be highly implicated in mushroom nutrition and in substrate bleaching during cultivation...

The release of H2O2 by A. bisporus was demonstrated during incubation of mycelium grown on cereal grains (commercial spawn) for 2–6 h in water, 11 and the presence of H2O2 in compost colonized by A. bisporus has been reported...

In the present study, H2O2 concentration increased during the vegetative growth for all the strains of A. bisporus and Pleurotus spp. and reached maximal values during the fruiting stages..."


Effects of temperature and hydrogen peroxide on mycelial growth of eight Pleurotus strains. Zharare et al., 2010.

"The highest hydrogen peroxide concentrations 0.016% (v/v) and 0.025% (v/v) in which bacteria and fungi, respectively, were observed to grow were within the concentration range 0.009–0.028% (v/v) that was found in Experiment 2 to cause a 50% reduction in mycelia growth in six of the eight Pleurotus strains tested. Use of hydrogen peroxide as a chemical sterilant in conjunction with strains highly tolerant of its toxicity offers a very cheap method of producing spawn as well as the mushrooms, and opens up opportunities for poor rural people."


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Important Links.

Be kind to others.

Edited by joze (02/28/24 02:06 PM)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: joze]
    #28679825 -

Well fuckin aye, call me a poor rural person then! 🤣 I don’t think i would take it so far as to attempting to sterilize spawn in such a manner but that’s certainly interesting!

I’m also very curious as to them mentioning P. baeocystis in a grow! I’ll have to read these articles later when I have more time but I hope they go into more depth about baeo haha. I have a culture of it now (not meaning to get off subject.)

You’re certainly better at using the internet than I am for find studies and I appreciate you for sharing all of this. It’s most interesting about the post harvest immersion of fruits in hydrogen peroxide. And the results concluded, which was shown to be able to store fresh fruits longer 🤔 I think this would particularly be of use to harvesting wild specimens for cloning or even swabbing possibly!

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28679828 -

Quote:

SupaThaRipper said:
You’re certainly better at using the internet than I am for find studies and I appreciate you for sharing all of this.



Happy to help, I like this thread!

Research is part of my job so I've definitely become pretty quick at it. But honestly, the hardest part is figuring out which keywords you need to use. Sometimes it takes five or six different combinations of keywords before you get the results you want.

For actual scientific research like this, I start with Google Scholar. I just searched "hydrogen peroxide mushroom cultivation" and those were some of the results from the first page.


--------------------


Important Links.

Be kind to others.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: joze]
    #28679842 -

Wow that’s great! I didn’t even know that existed! My job is operating heavy equipment for construction 😂

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28679848 -

Quote:

SupaThaRipper said:
Wow that’s great! I didn’t even know that existed! My job is operating heavy equipment for construction 😂


Hey that's not so bad. Better than pouring concrete :rofl:

Science actually kinda fucking sucks. A lot of scientists are underpaid and overworked. I'm trying to switch to a job in tech manufacturing.


--------------------


Important Links.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: joze]
    #28679862 -

Oh no doubt. Concrete would suck ass! Lol My job pays fairly well but only because it’s union otherwise I’d be getting fucked too! A more science focused job would be far more interesting than digging and working on utilities and foundations though! 🙄

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28679872 -

My dad did concrete for a decade or so.  His body is fucking jacked from it.  Multiple shoulder surgeries, joint replacements, constant pain now that he's in his 70s.

My brother ran a jack hammer for six months, 12+ hour days.  Permanent nerve damage.

Also, thanks for the Google scholar link.  I love digging uo research, but have been using search engines to do it the last two decades :lol:

And sorry for adding to the derail :rofl:  Thought twice about commenting but OP was already sidetracked!


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: tree frog]
    #28680005 -

Yeah exactly fuck concrete man. Those guys get beat to hell doing that. I don’t envy it one bit lol. A slab or stoop at my house is as far as I go lol. And running a jack hammer is base pay with life long consequences. Thats terrible to hear about that man 😢

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28680166 -

Errrgh, did concreting and bricklaying for a couple years along with demolition, my body is still fucked up.


--------------------

Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] * 1
    #28680178 -

Yeah that’s why you look about 80 now 🤣 🤣

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28680312 -

Ouch.
Not where it counts though
:biden:


--------------------

Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M]
    #28680650 -

😂

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28680906 -

Something I forgot about that I used to do when I ran a Martha. I would periodically put some H2O2 in the ultrasonic. It helped with the inevitable musty smell inside of my piping that ran into the tent.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28681019 -

Did it seem to positively or negatively affect your grows?

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28681565 -

Quote:

SupaThaRipper said:
I’m glad you said that! I have some subtropicalis fruits ready to get yanked right now. I’ll throw an entire fruit body on a plate and see what happens!

I’ll update the original post above in a few minutes!

Edit: I’ll have some pans to run in a couple weeks! Subtropicalis is still way too big lol


Well dammit, look what you made me go and do.

Cut off 2 x 1/2 inch sections of stipe just under the caps of a pan cyan kona and bunnell each. Dipped one of each in h2o2 then placed each of the 4 onto their own brf puck. Lets see how they each recover and how clean they run.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: ReverendMyc]
    #28681621 -

I’ll bet every single one will be clean as hell lol. Brf puck by itself is a damn miracle! 😂

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682169 -

Yes it definitely helped my grows.

I mainly use it for sanitary purposes.  It breaks down into water and oxygen. Isopropyl breaks down into acetone. It’s like $25 for a gallon of 12% that I can mix down into whatever I want it to be and it doesn’t have the additives that the first aid stuff has.

In the past I have sprayed it directly on surface bacteria and molds. If the tray is not completely bacterial or have a crazy trich infection it worked. However, if the tray is bad it’s not really worth the headache of waiting to see if it works. Full disclosure on that last part. My current projects don’t have issues so I have not needed to use it for that purpose in quite some time.

I have always wanted to do some experimenting just to see if it has any positive effects on the actual grow other than just sanitary purposes. I’m doing some tubs this weekend. I plan on making a few small tupperware (food storage) trays up to play with a little bit. I’m a contractor not a scientist but will do my best to log everything.

Let me know if y’all have any ideas on a process you might like to know about and I will try it. Right now I’m got plenty of spawn ready to go so I can spare a few quarts for experimenting.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28682178 -

Quote:

Mwj12977 said:
Something I forgot about that I used to do when I ran a Martha. I would periodically put some H2O2 in the ultrasonic. It helped with the inevitable musty smell inside of my piping that ran into the tent.



just... putting this out here,

h202 is wicked corosive.  depending on your model of US, it will eventually eat the stainless.


--------------------

The Hitchhiker's Guide To The Shroomery
My Cultivation Youtube Channel

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefExtreme]
    #28682193 -

I had pvc run through my tent. You are correct about it being corrosive. Really nasty stuff at high concentrations. If it gets on skin it turns it white and can burn if it’s too strong.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28682243 -

You know I’ve actually gotten pretty curious about using it to “sterilize” spawn since it was mentioned for “poor rural people” 🤣 I only have 3% (with additives, which I never even thought about before) I don’t know if you’d want to experiment and soak a few jars of spawn. Maybe the usual rinse of water and then a soak? I’ll try a few at 3% just for shits and giggles. Maybe a 12 hour soak idk. The only thing that concerns me about that is h2o2 breaking down into just water,  which would seem to encourage endospore germination.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682291 -

I will give that a try over the weekend. What my plans are is to soak 3 different jars with 3 different strengths. I’ll do 1%, 3% and 6% let them dry and do a g2g transfer. My wife will be happy I’m using my jars again as I’ve moved on to bags for growing myc. I’m just like stay out of my man cave and you won’t see them! 😂


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28682295 -

Hahaha I’ve been switching to bags a lot but I still like jars except for when it come time to break them up lol. I still like jars more for exotics though. I have probably a good couple hundred jars 🤣 pain in the ass to clean. I’m pretty much doubting any success at all with this method by a long shot but im still curious 🤷‍♂️ lol

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 2
    #28682301 -



--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77] * 2
    #28682496 -

im thinkin im use it in addition to soap for cleaning my tubs.    i dont see why not.  its quite affordable .


--------------------

The Hitchhiker's Guide To The Shroomery
My Cultivation Youtube Channel

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682507 -

bueno idea


--------------------
tail-lights, back-pockets, rearview mirrors

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: BeefExtreme]
    #28682732 -

Quote:

BeefSupremeJr said:
im thinkin im use it in addition to soap for cleaning my tubs.    i dont see why not.  its quite affordable .


Just wear your shitty old clothes, learnt that lesson the hard way.


--------------------

Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: 3.A.M] * 1
    #28682743 -

He mentioned something about working on something naked the other day. I like that idea more 🤣

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682753 -

😂 yeah that stuff will bleach your cloths and you definitely don’t want to work on it with your tips or nips out!


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682761 -

Quote:

SupaThaRipper said:
He mentioned something about working on something naked the other day. I like that idea more 🤣


That made my day too. There is just something unique in imagining a naked man working in a SAB 😂 I am going to give this a go when the temps get above 18°C, it's still a bit cold now.

ps. Yeah, we had 15-18°C for almost all of the winter, and I am just bragging 😎


--------------------
Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

Edited by Skropi (03/01/24 03:27 PM)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Skropi] * 1
    #28682777 -

Oh yeah man I’m in one of the coldest states. It’s been a very warm winter! Definitely one of my favorite winters, if that’s even possible to say lmao

@mwj, yeah I have a friend who strictly uses only h2o2, just store grade, but it has bleached his hair before lol

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682832 -

Post updated(with some wins and some losses)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682835 -

Quote:

SupaThaRipper said:
Haha at first I thought you were going to start hurting my feelings lol but I’m ecstatic to see you’re on board! I suppose I could add a drop of polysorbate(thanks Stipe,) as it does bead!



Yes. Use Tween 20.


Save the Dawn for pretreating straw.. when needed. Way too strong for anything less than large batches. Besides, you want to highly dilute most of the nasty stuff in it.


--------------------
"Luck favors the observant." - Workman


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: the_chosen_one]
    #28682848 -

Do you always wash your straw before cooking it? I’ve never actually done that but do still get some contamination here and there. I’m thinking I should give it a go!

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682877 -

I haven't in years thanks to good batches.. and microwaves :lol: but every once in a while you get that nasty batch. The dish soap thing was mostly for straw logs back in the day. That tek can be a little problematic for some. Probably why it's not as popular now a days. I have seen some people kill it though. It's all about a groove.


--------------------
"Luck favors the observant." - Workman


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: the_chosen_one]
    #28682900 -

Yeah that’s why I was asking I have seen anything recent on straw getting washed. Mine is store bought from the hardware store. Dry and free of most debris. Overall, visually, it looks well 🤔

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28682906 -

Yeah the mini bales are way better than a full bale. :thumbup:


--------------------
"Luck favors the observant." - Workman


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: ReverendMyc] * 2
    #28697924 -

Quote:

ReverendMyc said:
Quote:

SupaThaRipper said:
I’m glad you said that! I have some subtropicalis fruits ready to get yanked right now. I’ll throw an entire fruit body on a plate and see what happens!

I’ll update the original post above in a few minutes!

Edit: I’ll have some pans to run in a couple weeks! Subtropicalis is still way too big lol


Well dammit, look what you made me go and do.

Cut off 2 x 1/2 inch sections of stipe just under the caps of a pan cyan kona and bunnell each. Dipped one of each in h2o2 then placed each of the 4 onto their own brf puck. Lets see how they each recover and how clean they run.


Update to my little experiment on the pucks.

Nothing conclusive yet, but the non-h2o2 dipped stipes are recovering more quickly than the h2o2 dipped stipes on brf pucks.

The h2o2 may be cleaner, though. So I will need to do some transfers to agar to determine if that is the case. I will update again when I get back to my lab and get some more results.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic


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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: ReverendMyc] * 3
    #28698142 -

Awesome! 😁 glad you’re sharing your results! I’m hoping to get around to doing another update today. It’s been a few days!

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: PhenoDreamers] * 2
    #28700332 -

Updated! All experiments, but one, concluded! In my observation, h2o2 seems to be the best course of action for trichoderma in plate work. However brf still remains king when it comes to bacteria but it could always be aided with h2o2 if need be.

Also, I have some wild Ps. Heimii prints that are packed full of trich. I’ve been struggling to get clean germination without the aid of h2o2. I’ll be adding this experiment in at some point.

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: PhenoDreamers] * 3
    #28706486 -

Can't let this go yet.
I did some transfers from pucks to agar plates today so we will see if the h2o2 cloned did better. Looking at the brf puck jars before the transfers, you can see that the non-h2o2 dipped bits of stipe did recover faster but they also look like they may be much more bacterial. Check out the sweat on the jars on the left compared to the less grown out but cleaner looking myc on the h2o2 dipped right.


Interested to see if either transfer to agar will be bacterial. Thinking I will add the h2o2 dip as a suggestion in the brf puck tek thread for whole stipe piece transfers. Did two more that way today for some Pan Cambo Komodo.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic


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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: ReverendMyc] * 3
    #28723402 -

Now that I have a microscope, I’ll be posting experiments at a microscopic level! :naughty:

Many more cool tests coming!

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: PhenoDreamers] * 1
    #28724959 -

Anybody ever add it to hot Agar before pour?

Think that was an RR trick for dirty transfers or clones not recommended for germ as oxidizers are a main enemy of spores.

Before I Learned W.A. for bacterial issues, I used that with agar sandwiches successfully.

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: LewDoja] * 1
    #28730021 -

Hmmm I haven’t but I do use the fuck out of h2o2 on spores and haven’t had any issues with it. I’m willing to bet prolonged exposure to it would definitely cause cell damage at the least, if not just completely kill the spores

What is W.A.?

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: PhenoDreamers] * 1
    #28731650 -

Water agar.  Just no nutes agar


--------------------
A_wise_man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"


🎶  KIND TC's - by: Tri  🎶

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: LewDoja]
    #28734729 -

Ahhh yes, I stopped using water agar when I discovered brf. I might try it again for the extremely slow exotic species that seem to dislike nutrients

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: PhenoDreamers]
    #28734952 -

Quote:

SupaThaRipper said:
Ahhh yes, I stopped using water agar when I discovered brf. I might try it again for the extremely slow exotic species that seem to dislike nutrients


Never have done brf cake... bout time I tried one.

Edited by LewDoja (04/13/24 07:57 PM)

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Re: Supa’s LIVE/UNCUT H2O2 experiments! [Re: LewDoja]
    #28735321 -

I really don’t think there’s a better solution to bacteria right now out there

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 1
    #28745516 -



So I came back from out of town and had trich in one of my tubs. I suspect it came from over misting the day I left. It’s not the spawn because I’ve got two other tubs that have the same spawn and same coir that this one had.

Anyway for experimental purposes I hit it with 12% H202. It immediately foamed up and is definitely killing whatever it touches.

Sorry I didn’t get a pic before but it was green as fuck and triched out. I’ll keep updating the photos over the next week so we can see how it works out.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28745589 -

At that point it's the spawn or the coir. If you vetted the coir elsewhere, a jar of bad spawn snuck by you.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: LadysKnight]
    #28745637 -

Wasn’t just a jar. I use bags for my spawn and I premixed 3 tubs with all the same stuff. Shook it out in a sealed tub “beefy style”. 

The other two tubs are 100% good with equal pin grouping. With it being in the corner of the tub I can see the sub below it and there is myc going strong up the sidewall.

That’s why I’m saying it happened when I misted, I only misted that one tub because it caught a little bit too much direct sunlight the day before and my surface droplets dried up. I was in a hurry packing, in and out of the house, watering my house plants and messing around with the plants on my back deck. I’m pretty sure I contaminated it.

I get your point and would agree with you 100% of the time and say exactly what you said, but in this case I’ve got some evidence to back up my assumption.

Either way we get to see what 12% H202 does to trich


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77] * 2
    #28746346 -

If you think you can contaminate a tub by spraying water on it, who am I to try to convince you otherwise.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77] * 1
    #28746499 -

Quote:

Mwj12977 said:
So I came back from out of town and had trich in one of my tubs. I suspect it came from over misting the day I left. It’s not the spawn because I’ve got two other tubs that have the same spawn and same coir that this one had.



You either prepped your coir in a bucket/pot that has non-sterile grain starches or you have shit spawn. There isn't a mechanism available for a spray bottle filled with water to contaminate your tub unless the bottle contains the same kind of nutrient rich material.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: Stipe-n Cap] * 1
    #28746563 -

Yeah I just looked at the bottom of the tub and it’s got trich in it. I am gracefully admitting defeat. I’m still going to let it ride to see what happens to that shit I sprayed on the surface.

I must not have done a thorough job cleaning the tub in between projects. The other two are clean from top to bottom.

Thanks for helping me think clearly guys


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

Edited by OLDDAD77 (04/23/24 12:32 PM)

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: OLDDAD77]
    #28763847 -

Recently had someone ask me about trich stained tubs. I believe this could be possible. I’m very highly disgusted by the possibility but I’m going to monitor this to some degree and see if I can come up with something conclusive.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers]
    #28764916 -

That's when the chlorox comes out play.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: LewDoja]
    #28767638 -

That’s what I would think lol. Nothing gets past bleach, but does the porous surface trap trich within the pockets 🤔

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: PhenoDreamers] * 2
    #28768597 -

I've had some gnarly tubs before they've gotten the bleach bath and they've have performed as they should.

But that's a good experiment for that new scope you got. If that's feasible, might need to destroy a tricked out tub, in the name of science.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: LewDoja] * 2
    #28768754 -

Quote:

LewDoja said:
I've had some gnarly tubs before they've gotten the bleach bath and they've have performed as they should.

But that's a good experiment for that new scope you got. If that's feasible, might need to destroy a tricked out tub, in the name of science.


If you don't I will.
:slant::slant::slant::slant::slant::slant::slant::petri::slant::slant::slant::slant:


--------------------
The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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Re: Supa’s LIVE/UNCUT h202 experiments! [Re: vicepope]
    #28768766 -

Yes please do hahahahahha

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