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Offlinepastroomi
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Registered: 10/06/23
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Is this contaminated?
    #28676946 - 02/26/24 01:33 PM (10 months, 15 days ago)

Outside of “well now it is since you opened it” posts - does this look like contamination to you?

This is WBS inoculated with Agar. I only inoculated with one piece, so it’s interesting that it’s started from another part of the jar (unless a piece broke off in transfer or something?)
It’s also slightly off colored, but not obviously so. I’ve attached pics of the “healthy” side as well for comparison.

Any opinions?





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Edited by pastroomi (02/26/24 01:33 PM)

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InvisibleTPython
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Registered: 12/01/12
Posts: 860
Re: Is this contaminated? [Re: pastroomi]
    #28676956 - 02/26/24 01:41 PM (10 months, 15 days ago)

Mold

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Offlinepastroomi
The Sleeper
I'm a teapot

Registered: 10/06/23
Posts: 41
Loc: In Bed
Last seen: 15 days, 6 hours
Re: Is this contaminated? [Re: TPython]
    #28676976 - 02/26/24 02:07 PM (10 months, 15 days ago)

Quote:

TPython said:
Mold




Boo. I figured but didn’t want to admit it to myself.

Anything I can do? Or just toss the jar?


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OfflineYahra
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Registered: 03/06/23
Posts: 2,810
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Re: Is this contaminated? [Re: pastroomi]
    #28676980 - 02/26/24 02:13 PM (10 months, 15 days ago)

This is not salvagable, unfortunately.

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Offlinepastroomi
The Sleeper
I'm a teapot

Registered: 10/06/23
Posts: 41
Loc: In Bed
Last seen: 15 days, 6 hours
Re: Is this contaminated? [Re: Yahra]
    #28676982 - 02/26/24 02:14 PM (10 months, 15 days ago)

Sad, but understandable.

Thank you both for confirmation(s)!


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InvisibleTPython
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Registered: 12/01/12
Posts: 860
Re: Is this contaminated? [Re: pastroomi]
    #28676989 - 02/26/24 02:21 PM (10 months, 15 days ago)

Yeah your best bet is to trash it, especially now that it’s been opened. If you're out of genetic material you could attempt to transfer a small piece of that healthy myc back to agar to clean it up, but if that mold is already sporulating that might be more work than it’s worth.

You’re on the right track with agar to grain tho and that myc you were growing looks like some good stuff. Something probably just snuck in while you were making your transfer. Happens to the best of us

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