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Offlinetree frog
pan princess
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Registered: 09/14/23
Posts: 2,442
Loc: everywhere it rains
Last seen: 11 hours, 41 minutes
(Re)fruiting Spent Substrates. * 1
    #28659301 - 02/14/24 07:29 AM (10 months, 27 days ago)

Running a bit of an experiment and the active thread on the grain mix patent made me decide to share.

So, I was reading a paper recently about psilocybin bio-synthesis.  What routes and precursors actives use to make psilocybin.  I know in the past people have tried adding precursors, and it didn't seem to do anything special.

But, my thought is, spent substrates should have a lot of left over precursors.  And they may be in a more bioavailable form (for the mycelia) than whatever people were trying before.

Plus, I can recycle more substrate before feeding it to my worms.

Currently, I have a Pan Cyan clone fruiting on spent Bisporus substrate.  The sub was brought back to field capacity and pasteurized at 160.

The pins may be dryer than my last run of this culture.  Or, they may just be bluer.

Anyway, I'll have to invest in some potency tests and do several side by sides to prove anything.

But, I figured I'd post this, so people can post holes in my hypothesis.  Or play around with it themselves if they want.

Some pictures.

My last grow, on poo coir verm and grains.  Took two days to run.  Two days to start pinning after casing.  4 cup container.  My casing was too wet, so I got mostly pinning on the edges.  Second flush was better.



Same flush grown up.


Current grow.  On just grains and spent poo, coir, verm, and grains from a Bisporus grow.  Ten cup container.  The casing is more level with the substrate.  So maybe they're drying out and that's why.  But, these pins are super blue compared to the last grow.  Cased at fruiting.  Five days to pins.

Cluster on the left in light, cluster on the right in shadow.  To really show how blue they are.  Also, all of them are this color.  So if it's drying out or bruising, it's happening to the whole substrate.



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Edited by tree frog (02/14/24 07:38 AM)

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Offlinetree frog
pan princess
 User Gallery


Registered: 09/14/23
Posts: 2,442
Loc: everywhere it rains
Last seen: 11 hours, 41 minutes
Re: (Re)fruiting Spent Substrates. [Re: tree frog]
    #28675725 - 02/25/24 04:10 PM (10 months, 16 days ago)

Well this experiment seemed to be a success.  At least with this aggressive pan cyan culture.

The second flesh was kind of meddling but the third flesh was nice it's in the dehydrator right now.  I haven't sampled any of the fruits yet.

And the substrate looks like it's still got a lot of nutrition in it so I dunked it and it's back in the fruiting chamber I'll see what it does.

Not sure how it will work with other pans.

But I have a bunch more spent substrate pasteurizing right now and I'm about to find out how the bisporus likes it.

My only real concern is the leftover grains.  And my hope is that the biporus will be much more aggressive on this substrate than they are on coir.

So I'll be spawning poo jars as outlined in my signature to recycled substrate probably tomorrow.


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