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Sun-flare
🍄Padawan



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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28675482 - 02/25/24 01:14 PM (4 months, 1 day ago) |
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I have a couple of fully colonized jars, but I don’t have a free space in the fruiting chamber. Should I put them in the refrigerator or to store them at room temperature ?
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Bismillah
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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper] 5
#28675501 - 02/25/24 01:36 PM (4 months, 1 day ago) |
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Quote:
SupaThaRipper said:
Quote:
pablotx said: Ug. I feel like pounding my head on my desk... Everyone keeps talking about BVI growing really fast, but mine doesn't seem to be doing much. Any advice?
I swabbed 2 plates using a (purchased) spore print on 2/6/24. Here's what the plates look like today:

You can see in the right plate where I pulled 2 plugs for t1s on 2/11, photo today:

Is this just all mold?
That’s why I keep saying there’s a clear difference between ttbvi and bvi! The bvi culture I have is SLOW. The ttbvi I have is FAST
Is that the one I sent you? Growth has been slow for me as well compared to what others are reporting with TTBVI. I put some TTBVI spores on agar 3 days ago, curious to see how it will compare.
pablotx: Pan myc can do some weird shit, but that kinda knotting you have there doesn't look healthy. I had some plates that seemed to stall, but they colonized grain and fruited just fine.
I've been running some clones and MS in unmodified tubs, fruiting at 20-22C. The clones came from a 28C fruiting chamber, and they were not too impressed with these new conditions. Lots of aborts from misting as well. The best results came from sticking a small water tub in a mono, aquarium heater in the tub. Heater is set to 30, mono lid is cracked open. So the hot tub carries moist air up by convection, and as the humid air cools it sinks down on the trays next to the tub.
Here's a BVI clone tray from this mono:
 Those aborts are due to misting. I switched to a 10ml pipette to water the surface once or twice a day.
Cambodginiensis MS from the same mono:
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: RockinRobot]
#28675644 - 02/25/24 03:26 PM (4 months, 1 day ago) |
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Quote:
RockinRobot said:
Quote:
tree frog said: I have a bunch of worm castings sitting in my basement and am thinking about using my PC to pasteurize some of it this afternoon.
I have an induction burner that has a temperature read out and could set it to 160 and that lets me set a timer so if I can dial it in it should work pretty well.
Does anyone else pasteurize in their PC? I think my question mostly boils down to, lid on or off? Or maybe on but not latched?
Have you tried Rosco's Dehydrator pasteurization TEK? Been working great for me. I can do enough for 3-4 shoeboxes at 1 time with Black cow at 1.5 qt per shoebox.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=28297179&page=0&vc=1#28297179
Yeah brother.
This is the one I usually use. I'm just wanting to weigh my options for going bigger.
I could probably stuff everything I need in there though. I clipped one of my trays even to give myself more head room.
Also, reishiman, appreciate the feedback.
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28675662 - 02/25/24 03:35 PM (4 months, 1 day ago) |
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Quote:
Sun-flare said: I have a couple of fully colonized jars, but I don’t have a free space in the fruiting chamber. Should I put them in the refrigerator or to store them at room temperature ?
I'd leave them at room temperature or just move them to cooler room.
I imagine they survive being refrigerated but they probably wouldn't like it very much.
With my bisporus I think they actually would appreciate being left in the jar longer. But that's something I'm so experimenting with.
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 1
#28676643 - 02/26/24 08:07 AM (4 months, 8 hours ago) |
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Is this normal for tropicalis? Or is this garbage? It's on brown rice flour agar.
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pesa



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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves] 1
#28676744 - 02/26/24 09:54 AM (4 months, 6 hours ago) |
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brown rice is very high in nutrients, it will make mycelium go all puffy and swelly , use something with less nutrients get clear agar as possible,to me it looks clean i been using PDA(potato dextrose agar) forever and seen this kind of growth, a transfer on water agar or less nutrient agar will reveal what else is riding along if there are any contam. if you want to go for brown rice agar boil the brown rice, use coffee filter to remove all grains and use that water for agar, its same thing for potato agar never leave sediments in agar they will become feast for contams at some point.
pan mycelium goes full milkshake just like cubes when infected,
 this one is infected with all time sweet heart trich.
Edited by pesa (02/26/24 09:58 AM)
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: pesa]
#28676795 - 02/26/24 11:03 AM (4 months, 5 hours ago) |
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Okay thank you. I used the brown rice agar because I read it here somewhere....someone doing comparisons. I can easily try it on half strength lme agar to see what I get. Thanks again!
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Yahra
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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves]
#28676800 - 02/26/24 11:06 AM (4 months, 5 hours ago) |
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For the record, here are the BRF comparisons: Bod's BRF AGAR how to and "test" results
Seems like 1.5 % BRF is best.
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: Yahra] 1
#28676806 - 02/26/24 11:11 AM (4 months, 4 hours ago) |
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Yes, I think that was it. Thank you!
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pesa



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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves] 1
#28676813 - 02/26/24 11:17 AM (4 months, 4 hours ago) |
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pan mycelium does not care what its eating feed it bit extra nutrients and you are stuck with dilemma,is the milkshake clean , babayaga's water agar is very helpful on this. i started out with this
200ml solution
fresh potato 40g 20% dextorse 4g 2% agar 3g 1.5% highly nutritional
mushboy told me to cut it in half and that fixed it
200ml solution
fresh potato 20g 10% dextorse 3g 1.5% agar 6g 3 % go to recipe
 less thicc mycelium
what ever grow on plate, i pass it to water agar before going to grain, with potatoes you can mess around up to 10% with brf its 1.5% of entire solution
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: pesa]
#28676817 - 02/26/24 11:20 AM (4 months, 4 hours ago) |
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Okay, I will try your suggestion. Thanks for the help!
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Bismillah
Scharf im Schlafanzug


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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves]
#28676969 - 02/26/24 01:57 PM (4 months, 2 hours ago) |
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Quote:
autumn leaves said:


Is this normal for tropicalis? Or is this garbage? It's on brown rice flour agar.
Looks good! I've had growth like this on both BRF and LME, and got multiple flushes with pan cyan.
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: Bismillah]
#28677117 - 02/26/24 04:05 PM (4 months, 3 minutes ago) |
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Ok thanks!
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Yahra
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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves]
#28677125 - 02/26/24 04:10 PM (3 months, 30 days ago) |
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Quote:
autumn leaves said:

The red spots, what are these? I use BRF agar too and i've seen a couple on my plates too.
Thought it was contamination, but could this be overcooked BRF or something like this?
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A.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: Yahra] 1
#28677678 - 02/26/24 11:58 PM (3 months, 30 days ago) |
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I was thinking the same thing, I’ve never made brf plates but a couple times when my agar didn’t totally sterilize the plates grew tons of bacteria spots looking just like that.
But the myc looks ok so I’m assuming it’s brf chunk.
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LAGM2020     
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autumn leaves
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 1
#28677752 - 02/27/24 04:24 AM (3 months, 30 days ago) |
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They are pieces brf. I use an immersion blender to get it as clear as possible but it does the same thing as yeast....specks. Plus I put a little bit of pink color just so I would know which recipe these plates are. I think the specks picked up more of the dye
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MurphSmurf
Float On



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Re: Official Copelandia/Panaeolus Thread [Re: autumn leaves] 3
#28677794 - 02/27/24 06:31 AM (3 months, 30 days ago) |
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Got some ttBVI pop'n, took a pin clone from a center cluster.
I have started taking grain-to-agar every time I spawn-to-bulk as well, love how fast myc is at this point. It feels like myc is at full running speed by the end of colonization of grains, throwing a couple of those grains on agar allows them to keep running at high speeds. (Pictured is Pan v. PHV)
Doma of Magic Myco does this often, if I remember correctly he states there are other advantages to this as well. Don't recall what exactly though. Going to see if I can find his video on it.
Edited by MurphSmurf (02/27/24 06:35 AM)
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RockinRobot
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Re: Official Copelandia/Panaeolus Thread [Re: MurphSmurf] 12
#28677804 - 02/27/24 07:02 AM (3 months, 30 days ago) |
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Second flush TTBVI looking Great in the first tub. It should yield better than the first flush by far. Plan to harvest shortly. First flush on other 3 coming in nicely also.
These have turned out to be much easier and WAY faster than I had anticipated.



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MurphSmurf
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Re: Official Copelandia/Panaeolus Thread [Re: RockinRobot] 1
#28677860 - 02/27/24 07:59 AM (3 months, 30 days ago) |
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Quote:
RockinRobot said:

Beautiful!!!
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algae
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Re: Official Copelandia/Panaeolus Thread [Re: MurphSmurf]
#28678078 - 02/27/24 11:13 AM (3 months, 30 days ago) |
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Still trouble with the aborts. Switched over to a humidifier running half an hour every hour.
Should I pluck out all the tiny aborts?
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