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Offlinemushman509
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Registered: 02/16/24
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Day 20 no colonization - A.P.E using AIO bag.
    #28674216 - 02/24/24 01:22 PM (10 months, 17 days ago)



Image of bag ^

All of that is just moisture and it has puffed up with what I believe is gas. Anyways it is day 20 and i’m not seeing any signs of growth still. This is my first time. Everything was clean. I used an LC instead of a spore syringe. 78 degrees solid for the last while now… any clue as to what’s going on

Edited by mushman509 (02/24/24 02:15 PM)

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OfflineScrewup
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674243 - 02/24/24 01:46 PM (10 months, 17 days ago)

Stand back and stand by boys, I got this.

Read me and ask questions later.


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OfflineTheDibber
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674244 - 02/24/24 01:48 PM (10 months, 17 days ago)

That looks like alot of moisture for no colonization to me. I'm a novice so dont take my word for it. Are you using a heat matt? Also can you take a picture without the flash so we can see inside the bag properly :mushroom2:


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OfflineScrewup
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: TheDibber]
    #28674245 - 02/24/24 01:49 PM (10 months, 17 days ago)

The issue is you used untested LC in an AIO bag. Now read my previous link and ask questions later.


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Offlinemushman509
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Registered: 02/16/24
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: TheDibber]
    #28674275 - 02/24/24 02:17 PM (10 months, 17 days ago)

just updated with image

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Offlinemushman509
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: TheDibber]
    #28674279 - 02/24/24 02:22 PM (10 months, 17 days ago)

I believe I see a slight bit of white underneath the injection port. I could be wrong but after the image, I further inspected and there appears to be a bit of white on some of the contents.

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OfflineHazMatt
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674303 - 02/24/24 02:35 PM (10 months, 17 days ago)

First of all, I have learned my lesson with all in one bags. They suck. I’ve used that particular vendor as well. But that was when I was dumb and hadn’t found the Shroomery yet. I’d very much recommend that you read the Hitchhikers Guide to the Shroomery. It’s stickied in this forum. APE is slow growing and it is a failure in an all in one bag in my experience.

I will say that injecting bags is good for newbies as jumping right into agar can be intimidating. If you get another bag, just order a bag of straight up sterilized grain. You can get it from that vendor for way too much money. But it’s still a good option for noobs. Get yourself a grain bag and then get yourself a syringe of golden teachers. Golden teachers are easy to grow and forgiving of poor conditions. APE is not. Keep the APE bag. It might do something in a few months. Who knows.

Inject your grain bag with some golden teachers. Order yourself some coco coir on Amazon. Comes in bricks. Coco Bliss is what you want. Then go to Target and get yourself a 66qt Sterilite tub. The one with the purple handles. About $9. Then, bring that baby home and read the thread Pastywhyte’s EZ Dial Monotub. That’s all you need. That and some boiling water and a bucket. Lots of info here. I too made the all in one bag mistake early on. It worked with Golden Teachers. It did not with APE.

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Offlinemushman509
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: HazMatt]
    #28674310 - 02/24/24 02:42 PM (10 months, 17 days ago)

Quote:

HazMatt said:
First of all, I have learned my lesson with all in one bags. They suck. I’ve used that particular vendor as well. But that was when I was dumb and hadn’t found the Shroomery yet. I’d very much recommend that you read the Hitchhikers Guide to the Shroomery. It’s stickied in this forum. APE is slow growing and it is a failure in an all in one bag in my experience.

I will say that injecting bags is good for newbies as jumping right into agar can be intimidating. If you get another bag, just order a bag of straight up sterilized grain. You can get it from that vendor for way too much money. But it’s still a good option for noobs. Get yourself a grain bag and then get yourself a syringe of golden teachers. Golden teachers are easy to grow and forgiving of poor conditions. APE is not. Keep the APE bag. It might do something in a few months. Who knows.

Inject your grain bag with some golden teachers. Order yourself some coco coir on Amazon. Comes in bricks. Coco Bliss is what you want. Then go to Target and get yourself a 66qt Sterilite tub. The one with the purple handles. About $9. Then, bring that baby home and read the thread Pastywhyte’s EZ Dial Monotub. That’s all you need. That and some boiling water and a bucket. Lots of info here. I too made the all in one bag mistake early on. It worked with Golden Teachers. It did not with APE.




Thank you for your information!! I’ll try and do something of this sort for my next run. Where is a good place for Golden Teacher LC

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OfflineScrewup
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674328 - 02/24/24 03:11 PM (10 months, 17 days ago)

What strain you use in this case has zero to do with outcome…


Whether you buy an AIO or a grain bag itself…again has nothing to do with the outcome of untested LC or syringes and product you didn’t sterilize yourself.


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Edited by Screwup (02/24/24 03:14 PM)

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Offlinemushman509
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Registered: 02/16/24
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: Screwup]
    #28674365 - 02/24/24 03:44 PM (10 months, 17 days ago)

Quote:

Screwup said:
What strain you use in this case has zero to do with outcome…


Whether you buy an AIO or a grain bag itself…again has nothing to do with the outcome of untested LC or syringes and product you didn’t sterilize yourself.




So if i purchase another all in one bag… I need to test my Culture first to make sure it’s not contaminated, and if all goes well then am I free to inject into the grow bag? I would get from a different source, one that has grain and whatnot rather than millet mixed into all of it.

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OfflineScrewup
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674368 - 02/24/24 03:47 PM (10 months, 17 days ago)

If the LC is good can you shoot it into a bag? Sure. How do we know the bags good though?

It’s still subpar all things considered.


If you’re gonna do agar to test may as well just do everything yourself.


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Edited by Screwup (02/24/24 03:50 PM)

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Offlinemushman509
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Registered: 02/16/24
Posts: 10
Last seen: 10 months, 2 days
Re: Day 20 no colonization - A.P.E using AIO bag. [Re: Screwup]
    #28674387 - 02/24/24 03:57 PM (10 months, 17 days ago)

How do you feel about the inflatable monotubs, I don’t have the tools or resources for drilling holes into one of those plastic totes.

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OfflineScrewup
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674414 - 02/24/24 04:20 PM (10 months, 17 days ago)

So get some shoe boxes and offset the lid.
Use BRF instead as that will give you the best chance with spores or untested LC.

And again read that link I gave you.


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2023 Dehydrator TEK

Edited by Screwup (02/24/24 04:20 PM)

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OfflineHazMatt
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Registered: 12/17/23
Posts: 160
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: mushman509]
    #28674472 - 02/24/24 05:20 PM (10 months, 17 days ago)

Quote:

mushman509 said:
Quote:

HazMatt said:
First of all, I have learned my lesson with all in one bags. They suck. I’ve used that particular vendor as well. But that was when I was dumb and hadn’t found the Shroomery yet. I’d very much recommend that you read the Hitchhikers Guide to the Shroomery. It’s stickied in this forum. APE is slow growing and it is a failure in an all in one bag in my experience.

I will say that injecting bags is good for newbies as jumping right into agar can be intimidating. If you get another bag, just order a bag of straight up sterilized grain. You can get it from that vendor for way too much money. But it’s still a good option for noobs. Get yourself a grain bag and then get yourself a syringe of golden teachers. Golden teachers are easy to grow and forgiving of poor conditions. APE is not. Keep the APE bag. It might do something in a few months. Who knows.

Inject your grain bag with some golden teachers. Order yourself some coco coir on Amazon. Comes in bricks. Coco Bliss is what you want. Then go to Target and get yourself a 66qt Sterilite tub. The one with the purple handles. About $9. Then, bring that baby home and read the thread Pastywhyte’s EZ Dial Monotub. That’s all you need. That and some boiling water and a bucket. Lots of info here. I too made the all in one bag mistake early on. It worked with Golden Teachers. It did not with APE.




Thank you for your information!! I’ll try and do something of this sort for my next run. Where is a good place for Golden Teacher LC




I have bought like 30 different LC/spore syringes from [vendor removed]. I have had a 100% success rate with them too. Just injecting them right into grain bags. Testing it requires agar and it’s a pain in the ass. Especially when you’re new. I do that NOW, but in my opinion, would just be a frustrating way to start this hobby. If you get one from [vendor removed], inject it into a grain bag, your odds of failure are small. In my opinion, most strains are great and pretty easy. APE has been difficult for whatever reason. Not a good one to start with.

Get a grain bag and inject it. Then you’ll have about six weeks before it’s ready to spawn. In that time, learn about monotubs and substrate. I can’t recommend Pastywhyte’s EZ dial monotubs enough. It’s just stupid simple. I just spawn right into coco coir. I weigh the dry coco coir brick in grams. They’re around 600 grams. Multiply that weight by 4.6. 600x4.6 = 2760. You’ll boil 2760 milliliters of water and pour it into the bucket with the coco coir. Let it sit overnight. The moisture will be perfect. Mix it with your fully colonized spawn bag into your EZ dial tub and just put the lid on. You don’t need to touch it again until it’s time to harvest. There’s a little more detail to it than that. Read Pastywhyte’s thread. It’s just ridiculously easy. I have had a 100% success rate with this method. I don’t get contamination, never have failures. Just lots of shrooms.

If you decide you like this, you’ll want to start making your own grain bags and your own grain jars. Then you’ll want to be making your own agar trays as well. To do ANY of that, you’ll need either a pressure cooker or an autoclave. I want an autoclave but they’re $1,500. The one I want is anyways. Most of us use the Presto 23 quart pressure cooker. $125 on Amazon. Stupid easy. That Lressure cooker will last forever and it will handle ALL of your sterilization tasks, and making agar. When you’re ready to spend some more money on this hobby, get the pressure cooker.

But for now, just order a grain bag from somewhere like [vendor removed]. A grain bag might cost you like $30 after shipping. Pretty expensive. Then get a syringe of Golden Teacher from [vendor removed] and inject the bag. No testing. Just do it. Be clean about it and you’ll be fine. I never had a failure injecting grain bags with syringes from [vendor removed]. Not one failure. It’s a great way to start and you can grow from there. These days, I clone my best shrooms and use agar. The only things I really have to buy are while oats and coco coir. I have been using more jars instead of grain bags. But I can make grain bags for like $1.50 each. Thanks to my Presto pressure cooker!

Edited by Pastywhyte (02/24/24 07:05 PM)

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InvisiblePastywhyteMDiscord
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: HazMatt]
    #28674618 - 02/24/24 07:07 PM (10 months, 17 days ago)

Just a note, please don't mention vendor names in conjuction with growing actives. It may be an old rule and times may have changed, but it's ultimate purpose is to protect the site. Also please don't mention non sponsors here either please. We need our sponsors to feel they've got the site looking out for their interests and they keep this place going.

Thanks 😊

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OfflineHazMatt
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: Pastywhyte]
    #28674660 - 02/24/24 07:35 PM (10 months, 17 days ago)

Quote:

Pastywhyte said:
Just a note, please don't mention vendor names in conjuction with growing actives. It may be an old rule and times may have changed, but it's ultimate purpose is to protect the site. Also please don't mention non sponsors here either please. We need our sponsors to feel they've got the site looking out for their interests and they keep this place going.

Thanks 😊




Makes sense! Won’t happen again. I see you took care of it for me already. Thanks! I have like nine of your tubs going right now by the way. I really went nuts with this stuff. But your tek ALWAYS works for me. So good in fact, that I have more shrooms than I know what to do with.

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OfflineHazMatt
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: HazMatt]
    #28674684 - 02/24/24 07:47 PM (10 months, 17 days ago)

One more thing. I think people try starting out with APE because they hear that it’s the most potent. Lots of YouTube and Reddit talk about it. But trust me. Golden Teachers are PLENTY potent. For me, 3.5g of Golden Teachers is a pretty wild ride. I typically get 300 dried shrooms out of just ONE of Pasty’s EZ dial tubs. I do three flushes typically. 300g is going to last me like three years at least. And I have nine of these tubs going right now. One of them is APE. I’ve never tried it since my grow in a bag failure. In Pasty I have faith though. And the Shroomery. Start reading here. Start with the Hitchhikers Guide to the Shroomery. Lots of reading. All excellent info from very experienced growers.

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InvisiblePastywhyteMDiscord
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Re: Day 20 no colonization - A.P.E using AIO bag. [Re: HazMatt]
    #28674882 - 02/24/24 10:28 PM (10 months, 16 days ago)

Quote:

HazMatt said:
Quote:

Pastywhyte said:
Just a note, please don't mention vendor names in conjuction with growing actives. It may be an old rule and times may have changed, but it's ultimate purpose is to protect the site. Also please don't mention non sponsors here either please. We need our sponsors to feel they've got the site looking out for their interests and they keep this place going.

Thanks 😊




Makes sense! Won’t happen again. I see you took care of it for me already. Thanks! I have like nine of your tubs going right now by the way. I really went nuts with this stuff. But your tek ALWAYS works for me. So good in fact, that I have more shrooms than I know what to do with.




Glad the teks helped ya man, and your right, just grow more is always a good way to handle things

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Offlinemushman509
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Registered: 02/16/24
Posts: 10
Last seen: 10 months, 2 days
Re: Day 20 no colonization - A.P.E using AIO bag. [Re: Pastywhyte]
    #28675080 - 02/25/24 05:32 AM (10 months, 16 days ago)

Quote:

Pastywhyte said:
Quote:

HazMatt said:
Quote:

Pastywhyte said:
Just a note, please don't mention vendor names in conjuction with growing actives. It may be an old rule and times may have changed, but it's ultimate purpose is to protect the site. Also please don't mention non sponsors here either please. We need our sponsors to feel they've got the site looking out for their interests and they keep this place going.

Thanks 😊




Makes sense! Won’t happen again. I see you took care of it for me already. Thanks! I have like nine of your tubs going right now by the way. I really went nuts with this stuff. But your tek ALWAYS works for me. So good in fact, that I have more shrooms than I know what to do with.




Glad the teks helped ya man, and your right, just grow more is always a good way to handle things




Hey just like mat, thank you for informing me of proper etiquette! I’ll give your tek a try for next time I believe

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