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Mr Piggy
bioilliminicentยฉ๏ธ



Registered: 09/29/11
Posts: 9,701
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Quote:
Milonaire Mycology said: Oh its got the trich for sure. I didn't notice till after I posted. Eh, you don't grow mushies if you don't get contam. I'll probably really break the rules by just throwing that tray away. I had fun and got fruits, which was the ultimate goal. As far as totally avoiding contam, I like bagtek. Tubs I always get a bit of contam, probably mostly cause I grow in a human living environment instead of an isolated and well kept lab.
Homie, most of us grow in a human living environment. If you're getting contams in every tub it's you. Maybe you don't want to toot gordo's horn too loud while showing off a tub with only a few fruits and a patch of trich popping out.
-------------------- ๐
๐ด๐ฐ๐ผ ๐ต๐พ๐ธ๐ป New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 13,181
Loc: Hole
Last seen: 7 hours, 31 minutes
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Re: Post Your Cultivation Picture of The Day [Re: Mr Piggy] 14
#28674581 - 02/24/24 06:39 PM (4 months, 1 day ago) |
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Wombat TAT second flush. Swabbed and cloned. There will be a giveaway soon.
55g fruit. I done gone and ate it
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Mr Piggy
bioilliminicentยฉ๏ธ



Registered: 09/29/11
Posts: 9,701
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Re: Post Your Cultivation Picture of The Day [Re: Smellyhobbit]
#28674617 - 02/24/24 07:05 PM (4 months, 1 day ago) |
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Have fun!
-------------------- ๐
๐ด๐ฐ๐ผ ๐ต๐พ๐ธ๐ป New to cultivation or the shroomery? Observe the hitchhiker's guide The Hitchhiker's Guide to the Shroomery
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ibz
Lurker


Registered: 08/28/23
Posts: 179
Loc: United Kingdom
Last seen: 26 days, 22 hours
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Re: Post Your Cultivation Picture of The Day [Re: Smellyhobbit] 3
#28674657 - 02/24/24 07:32 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: Wombat TAT second flush. Swabbed and cloned. There will be a giveaway soon.
55g fruit. I done gone and ate it

ooo I have some WomTAT going aswell right now, ready to start pinning...i'll upload pics in the morning 
Juicy fruts
Edited by ibz (02/24/24 07:33 PM)
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mycoheadonaut
Stranger

Registered: 12/26/23
Posts: 86
Last seen: 24 days, 8 hours
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Re: Post Your Cultivation Picture of The Day [Re: Nichrome]
#28674746 - 02/24/24 08:44 PM (4 months, 1 day ago) |
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Quote:
Nichrome said:
Quote:
mycoheadonaut said: I meant are you using a light of some kind d to get that hue on the mycelium? Is that a bio luminescent mycelium?
The blue glow is "fluorescence" caused by UV exposure. The second shot is regular room light. It's translucent and has no pigment to the naked eye in regular light. Maybe a very slight transparent tan/khaki.
It's a nice clean culture of bacteria.
Very interesting I've always wanted to start growing other kinds of microbes especially bacteria for my soil used to date a soil scientist she would go on and on about the different bacteria that makes up truly good balanced soil. What's the name of that bacteria?
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Lemgrub



Registered: 04/02/18
Posts: 644
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Re: Post Your Cultivation Picture of The Day [Re: mycoheadonaut] 12
#28674755 - 02/24/24 08:51 PM (4 months, 1 day ago) |
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I'll cut these bad boys down tomorrow. Might take a lil 5g trip with a movie too
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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tholos
Stranger



Registered: 03/06/23
Posts: 564
Last seen: 4 days, 11 hours
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Re: Post Your Cultivation Picture of The Day [Re: Lemgrub] 10
#28674766 - 02/24/24 09:05 PM (4 months, 1 day ago) |
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LG Black Ring before the chop - 27qt sterlite

cloned + swabbed the fruit in the lower right hand corner of the photo
Edited by tholos (02/24/24 09:13 PM)
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: Post Your Cultivation Picture of The Day [Re: mycoheadonaut] 6
#28674831 - 02/24/24 09:52 PM (4 months, 1 day ago) |
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Quote:
mycoheadonaut said:
Quote:
Nichrome said:
Quote:
mycoheadonaut said: I meant are you using a light of some kind d to get that hue on the mycelium? Is that a bio luminescent mycelium?
The blue glow is "fluorescence" caused by UV exposure. The second shot is regular room light. It's translucent and has no pigment to the naked eye in regular light. Maybe a very slight transparent tan/khaki.
It's a nice clean culture of bacteria.
Very interesting I've always wanted to start growing other kinds of microbes especially bacteria for my soil used to date a soil scientist she would go on and on about the different bacteria that makes up truly good balanced soil. What's the name of that bacteria?
Narrowed down to the Pseudomonas fluoresens group. Probably a strain of fluorescens proper and not putida or others in the complex. The pyoverdine this one makes is extremely blue and the bacteria has little to no pigment otherwise, at least no other pigment visible in spectrum I can see. That's why I rule out putida which makes pyoverdine that is green tinted. I grow that one too. I swiped it from some fancy commercial microbial inoculant. This one I pulled off a wild cordyceps militaris fruit. Likely a strain of pseudomonas fluorescens based on the color of the reflection, and I was able to successfully pair it with slime mold amoebas which is a rarity in it's self.
Not all Pseudomonas fluorescens make fluorescent pyoverdine. Not all of them will pair with amoeba either. This one is also swarm motile on media as high as 2% agar as far as I have tested which is pretty dense for swarm motility to develop. Might be swarm motile on denser material too as I suspect.
It's on my to do list to get it sequenced. Took a while to get a single expression. It took a round of agar-lc-agar-agar-agar before I was able to narrow down the prettiest one. Some didn't fluoresce and some weren't as brilliant. This one is prime now and I'll keep it banked.
The amoebas may have helped out. wink wink. They are selective little buggers. The'll give me back the bacteria on it's own but they won't go off on their own without it. They take it with them everywhere. They equip spores with it. They love each other. Oh can't you see that it's blue.
This was the plate it transferred from which still had a little diversity.

Dropped a wedge of cube on this plate after the transfer was taken and the cube myc plowed right through it.
-------------------- question everything, it's very responsive โDrink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.โ โ Thich Nhat Hanh Gypsum
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: Post Your Cultivation Picture of The Day [Re: Smellyhobbit]
#28674835 - 02/24/24 09:53 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: Wombat TAT second flush. Swabbed and cloned. There will be a giveaway soon.
55g fruit. I done gone and ate it

-------------------- question everything, it's very responsive โDrink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.โ โ Thich Nhat Hanh Gypsum
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mycoheadonaut
Stranger

Registered: 12/26/23
Posts: 86
Last seen: 24 days, 8 hours
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Re: Post Your Cultivation Picture of The Day [Re: Nichrome]
#28674934 - 02/24/24 11:21 PM (4 months, 1 day ago) |
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The blue glow is "fluorescence" caused by UV exposure. The second shot is regular room light. It's translucent and has no pigment to the naked
Narrowed down to the Pseudomonas fluoresens group. Probably a strain of fluorescens proper and not putida or others in the complex. The pyoverdine this one makes is extremely blue and the bacteria has little to no pigment otherwise, at least no other pigment visible in spectrum I can see. That's why I rule out putida which makes pyoverdine that is green tinted. I grow that one too. I swiped it from some fancy commercial microbial inoculant. This one I pulled off a wild cordyceps militaris fruit. Likely a strain of pseudomonas fluorescens based on the color of the reflection, and I was able to successfully pair it with slime mold amoebas which is a rarity in it's self.
Not all Pseudomonas fluorescens make fluorescent pyoverdine. Not all of them will pair with amoeba either. This one is also swarm motile on media as high as 2% agar as far as I have tested which is pretty dense for swarm motility to develop. Might be swarm motile on denser material too as I suspect.
It's on my to do list to get it sequenced. Took a while to get a single expression. It took a round of agar-lc-agar-agar-agar before I was able to narrow down the prettiest one. Some didn't fluoresce and some weren't as brilliant. This one is prime now and I'll keep it banked.
The amoebas may have helped out. wink wink. They are selective little buggers. The'll give me back the bacteria on it's own but they won't go off on their own without it. They take it with them everywhere. They equip spores with it. They love each other. Oh can't you see that it's blue.
This was the plate it transferred from which still had a little diversity.

Dropped a wedge of cube on this plate after the transfer was taken and the cube myc plowed right through it.
Very nice after reading a little about them seems to be a major commercial player have you tried kings b media for it? Also are you using a bio cabinet or a flow hood for cultivation of bacterium and molds? Good idea on swiping some samples from those inoculants that are sky high in prices lol you should put some with plants and see if yours exhibit any of the rooting properties or the antibiotics made from it are also cool
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fiddle_head
sporophyte



Registered: 08/05/08
Posts: 2,788
Loc: shortbus
Last seen: 17 hours, 51 minutes
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Re: Post Your Cultivation Picture of The Day [Re: saralove] 15
#28674943 - 02/24/24 11:31 PM (4 months, 1 day ago) |
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tub update this culture always gets a little too excited too early
-------------------- lagm 2024 || lagm 2023 || 2023 || Things That Are Good ============= If you cannot afford petris you can still do this hobby. ๐ฟ Looking for spores? Look no further. ๐
XI ๐
๐ด ๐ฐ ๐ผ ๐ฒ ๐ป ๐ธ ๐ฝ ๐ถ ๐
๐
๐ฐ ๐ฟ XI
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mycoheadonaut
Stranger

Registered: 12/26/23
Posts: 86
Last seen: 24 days, 8 hours
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Re: Post Your Cultivation Picture of The Day [Re: fiddle_head]
#28674957 - 02/24/24 11:44 PM (4 months, 1 day ago) |
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Quote:
fiddle_head said: tub update this culture always gets a little too excited too early

Clusters looking grooot
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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 2,630
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Re: Post Your Cultivation Picture of The Day [Re: mycoheadonaut]
#28675088 - 02/25/24 05:49 AM (4 months, 1 day ago) |
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those clusters omg nice fiddldhead
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bjjrev
Stranger



Registered: 12/25/22
Posts: 248
Last seen: 2 months, 21 hours
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Re: Post Your Cultivation Picture of The Day [Re: rhizoRider] 4
#28675143 - 02/25/24 07:02 AM (4 months, 23 hours ago) |
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1 week old mono of Z strain... loving the rhizomorphic mycelium!! I've always used shoe boxes on previous grows and this time decided to try pastys ez dial monotub... I fucked up the hole diameter but it still seems to be rolling along fine!
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RockinRobot
Stranger


Registered: 12/08/22
Posts: 1,245
Last seen: 36 minutes, 10 seconds
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Re: Post Your Cultivation Picture of The Day [Re: bjjrev] 7
#28675151 - 02/25/24 07:14 AM (4 months, 22 hours ago) |
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TTBVI are really fast.
Second flush on first pic. First flush other 3 S2B 2/17.



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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 2,630
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Re: Post Your Cultivation Picture of The Day [Re: RockinRobot] 11
#28675169 - 02/25/24 07:31 AM (4 months, 22 hours ago) |
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HILLBILLY OUTLAW
Above And Beyond!


Registered: 04/21/22
Posts: 3,575
Loc: Luckenbach Texas
Last seen: 18 minutes, 26 seconds
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Re: Post Your Cultivation Picture of The Day [Re: saralove] 16
#28675175 - 02/25/24 07:35 AM (4 months, 22 hours ago) |
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Some metabolites in bottom corner. Who knows whatโs going on under that solid mass of craziness!
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๐ด ๐ฐ ๐ผ ๐ฒ ๐ป ๐ธ ๐ฝ ๐ถ ๐
๐
๐ฐ ๐ฟ TEAM SPREAD THE LOVE! Smellyhobbit said: Embarrassment and bashfulness are leeches on your ability to learn.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,257
Loc: USA
Last seen: 33 minutes, 57 seconds
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Re: Post Your Cultivation Picture of The Day [Re: RockinRobot]
#28675176 - 02/25/24 07:35 AM (4 months, 22 hours ago) |
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Is this the same tub as before in which you had side pins? If not, what did you do differently?
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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 2,630
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Alien tub so awesome ๐ ๐
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,257
Loc: USA
Last seen: 33 minutes, 57 seconds
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Quote:
HILLBILLY OUTLAW said:

Some metabolites in bottom corner. Who knows whatโs going on under that solid mass of craziness!

Thatโs one nice mass! ๐๐๐
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